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Evaluating the Effects of Oral Morphine on Embryonic Development of Cerebellum in Wistar Rats

In the present research, the effect of morphine consumption during pregnancy on the development of the embryo's spinal cord was studied in Wistar rat. Female Wistar rats (Wt: 250-300 g) were mated with males. The test group received morphine (0.01 mg/ml) in their drinking water. Pregnant rats w...

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Autores principales: Niknam, Narges A., Azarnia, Mahnaz, Bahadoran, Hossein, Kazemi, Masoomeh, Tekieh, Elaheh, Ranjbaran, Mina, Sahraei, Hedayat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Neuroscience Society 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4202532/
https://www.ncbi.nlm.nih.gov/pubmed/25337339
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author Niknam, Narges A.
Azarnia, Mahnaz
Bahadoran, Hossein
Kazemi, Masoomeh
Tekieh, Elaheh
Ranjbaran, Mina
Sahraei, Hedayat
author_facet Niknam, Narges A.
Azarnia, Mahnaz
Bahadoran, Hossein
Kazemi, Masoomeh
Tekieh, Elaheh
Ranjbaran, Mina
Sahraei, Hedayat
author_sort Niknam, Narges A.
collection PubMed
description In the present research, the effect of morphine consumption during pregnancy on the development of the embryo's spinal cord was studied in Wistar rat. Female Wistar rats (Wt: 250-300 g) were mated with males. The test group received morphine (0.01 mg/ml) in their drinking water. Pregnant rats were later killed with chloroform on the 12th, 13th and 14th days of pregnancy, and the embryos were taken out surgically. The embryos were fixed in formalin 10% for 2 weeks. Then, the weight of fixed embryos was calculated by a scale. In addition, several animals’ sizes including fronto-posterior and lateral length were measured by a caliper. Tissue processing, sectioning and hematoxylin and eosin (H&E) staining were applied for the embryos. The sections were examined for spinal cord development by light microscope and MOTIC software. Significant decrease was observed in the fronto-posterior and lateral length and the weight of the embryos in the test groups. The thickness of the white matter layer decreased on the 12th, 13th and 14th embryonic days. The thickness of the spine's grey layer was also less than the control group, on the same days. Increase in the length of the ependimal duct observed as well. Number of grey substance cells decreased compared to the control group within the same days. Meanwhile, thickness of the germinal layer reduced in comparison to the control group on the mentioned days. In conclusion, morphine consumption during pregnancy causes defects in growth and completion of the spinal cord.
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spelling pubmed-42025322014-10-21 Evaluating the Effects of Oral Morphine on Embryonic Development of Cerebellum in Wistar Rats Niknam, Narges A. Azarnia, Mahnaz Bahadoran, Hossein Kazemi, Masoomeh Tekieh, Elaheh Ranjbaran, Mina Sahraei, Hedayat Basic Clin Neurosci Research Papers In the present research, the effect of morphine consumption during pregnancy on the development of the embryo's spinal cord was studied in Wistar rat. Female Wistar rats (Wt: 250-300 g) were mated with males. The test group received morphine (0.01 mg/ml) in their drinking water. Pregnant rats were later killed with chloroform on the 12th, 13th and 14th days of pregnancy, and the embryos were taken out surgically. The embryos were fixed in formalin 10% for 2 weeks. Then, the weight of fixed embryos was calculated by a scale. In addition, several animals’ sizes including fronto-posterior and lateral length were measured by a caliper. Tissue processing, sectioning and hematoxylin and eosin (H&E) staining were applied for the embryos. The sections were examined for spinal cord development by light microscope and MOTIC software. Significant decrease was observed in the fronto-posterior and lateral length and the weight of the embryos in the test groups. The thickness of the white matter layer decreased on the 12th, 13th and 14th embryonic days. The thickness of the spine's grey layer was also less than the control group, on the same days. Increase in the length of the ependimal duct observed as well. Number of grey substance cells decreased compared to the control group within the same days. Meanwhile, thickness of the germinal layer reduced in comparison to the control group on the mentioned days. In conclusion, morphine consumption during pregnancy causes defects in growth and completion of the spinal cord. Iranian Neuroscience Society 2013 /pmc/articles/PMC4202532/ /pubmed/25337339 Text en Copyright © 2013 Iranian Neuroscience Society http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Research Papers
Niknam, Narges A.
Azarnia, Mahnaz
Bahadoran, Hossein
Kazemi, Masoomeh
Tekieh, Elaheh
Ranjbaran, Mina
Sahraei, Hedayat
Evaluating the Effects of Oral Morphine on Embryonic Development of Cerebellum in Wistar Rats
title Evaluating the Effects of Oral Morphine on Embryonic Development of Cerebellum in Wistar Rats
title_full Evaluating the Effects of Oral Morphine on Embryonic Development of Cerebellum in Wistar Rats
title_fullStr Evaluating the Effects of Oral Morphine on Embryonic Development of Cerebellum in Wistar Rats
title_full_unstemmed Evaluating the Effects of Oral Morphine on Embryonic Development of Cerebellum in Wistar Rats
title_short Evaluating the Effects of Oral Morphine on Embryonic Development of Cerebellum in Wistar Rats
title_sort evaluating the effects of oral morphine on embryonic development of cerebellum in wistar rats
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4202532/
https://www.ncbi.nlm.nih.gov/pubmed/25337339
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