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Functional cardiac imaging by random access microscopy

Advances in the development of voltage sensitive dyes and Ca(2+) sensors in combination with innovative microscopy techniques allowed researchers to perform functional measurements with an unprecedented spatial and temporal resolution. At the moment, one of the shortcomings of available technologies...

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Autores principales: Crocini, Claudia, Coppini, Raffaele, Ferrantini, Cecilia, Pavone, Francesco S., Sacconi, Leonardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4202699/
https://www.ncbi.nlm.nih.gov/pubmed/25368580
http://dx.doi.org/10.3389/fphys.2014.00403
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author Crocini, Claudia
Coppini, Raffaele
Ferrantini, Cecilia
Pavone, Francesco S.
Sacconi, Leonardo
author_facet Crocini, Claudia
Coppini, Raffaele
Ferrantini, Cecilia
Pavone, Francesco S.
Sacconi, Leonardo
author_sort Crocini, Claudia
collection PubMed
description Advances in the development of voltage sensitive dyes and Ca(2+) sensors in combination with innovative microscopy techniques allowed researchers to perform functional measurements with an unprecedented spatial and temporal resolution. At the moment, one of the shortcomings of available technologies is their incapability of imaging multiple fast phenomena while controlling the biological determinants involved. In the near future, ultrafast deflectors can be used to rapidly scan laser beams across the sample, performing optical measurements of action potential and Ca(2+) release from multiple sites within cardiac cells and tissues. The same scanning modality could also be used to control local Ca(2+) release and membrane electrical activity by activation of caged compounds and light-gated ion channels. With this approach, local Ca(2+) or voltage perturbations could be induced, simulating arrhythmogenic events, and their impact on physiological cell activity could be explored. The development of this optical methodology will provide fundamental insights in cardiac disease, boosting new therapeutic strategies, and, more generally, it will represent a new approach for the investigation of the physiology of excitable cells.
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spelling pubmed-42026992014-11-03 Functional cardiac imaging by random access microscopy Crocini, Claudia Coppini, Raffaele Ferrantini, Cecilia Pavone, Francesco S. Sacconi, Leonardo Front Physiol Physiology Advances in the development of voltage sensitive dyes and Ca(2+) sensors in combination with innovative microscopy techniques allowed researchers to perform functional measurements with an unprecedented spatial and temporal resolution. At the moment, one of the shortcomings of available technologies is their incapability of imaging multiple fast phenomena while controlling the biological determinants involved. In the near future, ultrafast deflectors can be used to rapidly scan laser beams across the sample, performing optical measurements of action potential and Ca(2+) release from multiple sites within cardiac cells and tissues. The same scanning modality could also be used to control local Ca(2+) release and membrane electrical activity by activation of caged compounds and light-gated ion channels. With this approach, local Ca(2+) or voltage perturbations could be induced, simulating arrhythmogenic events, and their impact on physiological cell activity could be explored. The development of this optical methodology will provide fundamental insights in cardiac disease, boosting new therapeutic strategies, and, more generally, it will represent a new approach for the investigation of the physiology of excitable cells. Frontiers Media S.A. 2014-10-20 /pmc/articles/PMC4202699/ /pubmed/25368580 http://dx.doi.org/10.3389/fphys.2014.00403 Text en Copyright © 2014 Crocini, Coppini, Ferrantini, Pavone and Sacconi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Crocini, Claudia
Coppini, Raffaele
Ferrantini, Cecilia
Pavone, Francesco S.
Sacconi, Leonardo
Functional cardiac imaging by random access microscopy
title Functional cardiac imaging by random access microscopy
title_full Functional cardiac imaging by random access microscopy
title_fullStr Functional cardiac imaging by random access microscopy
title_full_unstemmed Functional cardiac imaging by random access microscopy
title_short Functional cardiac imaging by random access microscopy
title_sort functional cardiac imaging by random access microscopy
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4202699/
https://www.ncbi.nlm.nih.gov/pubmed/25368580
http://dx.doi.org/10.3389/fphys.2014.00403
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