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Antigen Transfer from Exosomes to Dendritic Cells as an Explanation for the Immune Enhancement Seen by IgE Immune Complexes

IgE antigen complexes induce increased specific T cell proliferation and increased specific IgG production. Immediately after immunization, CD23(+) B cells capture IgE antigen complexes, transport them to the spleen where, via unknown mechanisms, dendritic cells capture the antigen and present it to...

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Autores principales: Martin, Rebecca K., Brooks, Keith B., Henningsson, Frida, Heyman, Birgitta, Conrad, Daniel H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4203810/
https://www.ncbi.nlm.nih.gov/pubmed/25330118
http://dx.doi.org/10.1371/journal.pone.0110609
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author Martin, Rebecca K.
Brooks, Keith B.
Henningsson, Frida
Heyman, Birgitta
Conrad, Daniel H.
author_facet Martin, Rebecca K.
Brooks, Keith B.
Henningsson, Frida
Heyman, Birgitta
Conrad, Daniel H.
author_sort Martin, Rebecca K.
collection PubMed
description IgE antigen complexes induce increased specific T cell proliferation and increased specific IgG production. Immediately after immunization, CD23(+) B cells capture IgE antigen complexes, transport them to the spleen where, via unknown mechanisms, dendritic cells capture the antigen and present it to T cells. CD23, the low affinity IgE receptor, binds IgE antigen complexes and internalizes them. In this study, we show that these complexes are processed onto B-cell derived exosomes (bexosomes) in a CD23 dependent manner. The bexosomes carry CD23, IgE and MHC II and stimulate antigen specific T-cell proliferation in vitro. When IgE antigen complex stimulated bexosomes are incubated with dendritic cells, dendritic cells induce specific T-cell proliferation in vivo, similar to IgE antigen complexes. This suggests that bexosomes can provide the essential transfer mechanism for IgE antigen complexes from B cells to dendritic cells.
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spelling pubmed-42038102014-10-27 Antigen Transfer from Exosomes to Dendritic Cells as an Explanation for the Immune Enhancement Seen by IgE Immune Complexes Martin, Rebecca K. Brooks, Keith B. Henningsson, Frida Heyman, Birgitta Conrad, Daniel H. PLoS One Research Article IgE antigen complexes induce increased specific T cell proliferation and increased specific IgG production. Immediately after immunization, CD23(+) B cells capture IgE antigen complexes, transport them to the spleen where, via unknown mechanisms, dendritic cells capture the antigen and present it to T cells. CD23, the low affinity IgE receptor, binds IgE antigen complexes and internalizes them. In this study, we show that these complexes are processed onto B-cell derived exosomes (bexosomes) in a CD23 dependent manner. The bexosomes carry CD23, IgE and MHC II and stimulate antigen specific T-cell proliferation in vitro. When IgE antigen complex stimulated bexosomes are incubated with dendritic cells, dendritic cells induce specific T-cell proliferation in vivo, similar to IgE antigen complexes. This suggests that bexosomes can provide the essential transfer mechanism for IgE antigen complexes from B cells to dendritic cells. Public Library of Science 2014-10-20 /pmc/articles/PMC4203810/ /pubmed/25330118 http://dx.doi.org/10.1371/journal.pone.0110609 Text en © 2014 Martin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Martin, Rebecca K.
Brooks, Keith B.
Henningsson, Frida
Heyman, Birgitta
Conrad, Daniel H.
Antigen Transfer from Exosomes to Dendritic Cells as an Explanation for the Immune Enhancement Seen by IgE Immune Complexes
title Antigen Transfer from Exosomes to Dendritic Cells as an Explanation for the Immune Enhancement Seen by IgE Immune Complexes
title_full Antigen Transfer from Exosomes to Dendritic Cells as an Explanation for the Immune Enhancement Seen by IgE Immune Complexes
title_fullStr Antigen Transfer from Exosomes to Dendritic Cells as an Explanation for the Immune Enhancement Seen by IgE Immune Complexes
title_full_unstemmed Antigen Transfer from Exosomes to Dendritic Cells as an Explanation for the Immune Enhancement Seen by IgE Immune Complexes
title_short Antigen Transfer from Exosomes to Dendritic Cells as an Explanation for the Immune Enhancement Seen by IgE Immune Complexes
title_sort antigen transfer from exosomes to dendritic cells as an explanation for the immune enhancement seen by ige immune complexes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4203810/
https://www.ncbi.nlm.nih.gov/pubmed/25330118
http://dx.doi.org/10.1371/journal.pone.0110609
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