Development and Evaluation of a Novel and Rapid Detection Assay for Botrytis cinerea Based on Loop-Mediated Isothermal Amplification

Botrytis cinerea is a devastating plant pathogen that causes grey mould disease. In this study, we developed a visual detection method of B. cinerea based on the Bcos5 sequence using loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue dye (HNB). The LAMP reaction was optimal at 6...

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Detalles Bibliográficos
Autores principales: Duan, Ya-Bing, Ge, Chang-Yan, Zhang, Xiao-Ke, Wang, Jian-Xin, Zhou, Ming-Guo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4203854/
https://www.ncbi.nlm.nih.gov/pubmed/25329402
http://dx.doi.org/10.1371/journal.pone.0111094

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4203854/
https://www.ncbi.nlm.nih.gov/pubmed/25329402
http://dx.doi.org/10.1371/journal.pone.0111094

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