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A novel method to follow meiotic progression in Arabidopsis using confocal microscopy and 5-ethynyl-2′-deoxyuridine labeling

BACKGROUND: Meiosis progression in the more recent past has been investigated using 5-bromo-2′-deoxyuridine (BrdU) uptake by S-phase meiocytes undergoing DNA replication. BrdU uptake is detected by reaction with BrdU antibody followed by epifluorescent microscopy examination of chromosome spreads an...

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Detalles Bibliográficos
Autores principales:	Stronghill, Patti E, Azimi, Wajma, Hasenkampf, Clare A
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4203904/ https://www.ncbi.nlm.nih.gov/pubmed/25337148 http://dx.doi.org/10.1186/1746-4811-10-33

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