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Improvement of an antibody-enzyme coupling yield by enzyme surface supercharging
BACKGROUND: Protein cross-coupling reactions demand high yields, especially if the products are intended for bioanalytics, like enzyme-linked immunosorbent assays. Amongst other factors, the coupling yield depends on the concentration of the proteins being used for coupling. Protein supercharging of...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4203919/ https://www.ncbi.nlm.nih.gov/pubmed/25326050 http://dx.doi.org/10.1186/s12896-014-0088-6 |