Cargando…

Progressive replacement of embryo-derived cardiac macrophages with age

Cardiac macrophages (cMΦ) are critical for early postnatal heart regeneration and fibrotic repair in the adult heart, but their origins and cellular dynamics during postnatal development have not been well characterized. Tissue macrophages can be derived from embryonic progenitors or from monocytes...

Descripción completa

Detalles Bibliográficos
Autores principales: Molawi, Kaaweh, Wolf, Yochai, Kandalla, Prashanth K., Favret, Jeremy, Hagemeyer, Nora, Frenzel, Kathrin, Pinto, Alexander R., Klapproth, Kay, Henri, Sandrine, Malissen, Bernard, Rodewald, Hans-Reimer, Rosenthal, Nadia A., Bajenoff, Marc, Prinz, Marco, Jung, Steffen, Sieweke, Michael H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4203946/
https://www.ncbi.nlm.nih.gov/pubmed/25245760
http://dx.doi.org/10.1084/jem.20140639
_version_ 1782340469655601152
author Molawi, Kaaweh
Wolf, Yochai
Kandalla, Prashanth K.
Favret, Jeremy
Hagemeyer, Nora
Frenzel, Kathrin
Pinto, Alexander R.
Klapproth, Kay
Henri, Sandrine
Malissen, Bernard
Rodewald, Hans-Reimer
Rosenthal, Nadia A.
Bajenoff, Marc
Prinz, Marco
Jung, Steffen
Sieweke, Michael H.
author_facet Molawi, Kaaweh
Wolf, Yochai
Kandalla, Prashanth K.
Favret, Jeremy
Hagemeyer, Nora
Frenzel, Kathrin
Pinto, Alexander R.
Klapproth, Kay
Henri, Sandrine
Malissen, Bernard
Rodewald, Hans-Reimer
Rosenthal, Nadia A.
Bajenoff, Marc
Prinz, Marco
Jung, Steffen
Sieweke, Michael H.
author_sort Molawi, Kaaweh
collection PubMed
description Cardiac macrophages (cMΦ) are critical for early postnatal heart regeneration and fibrotic repair in the adult heart, but their origins and cellular dynamics during postnatal development have not been well characterized. Tissue macrophages can be derived from embryonic progenitors or from monocytes during inflammation. We report that within the first weeks after birth, the embryo-derived population of resident CX3CR1(+) cMΦ diversifies into MHCII(+) and MHCII(−) cells. Genetic fate mapping demonstrated that cMΦ derived from CX3CR1(+) embryonic progenitors persisted into adulthood but the initially high contribution to resident cMΦ declined after birth. Consistent with this, the early significant proliferation rate of resident cMΦ decreased with age upon diversification into subpopulations. Bone marrow (BM) reconstitution experiments showed monocyte-dependent quantitative replacement of all cMΦ populations. Furthermore, parabiotic mice and BM chimeras of nonirradiated recipient mice revealed a slow but significant donor contribution to cMΦ. Together, our observations indicate that in the heart, embryo-derived cMΦ show declining self-renewal with age and are progressively substituted by monocyte-derived macrophages, even in the absence of inflammation.
format Online
Article
Text
id pubmed-4203946
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-42039462015-04-20 Progressive replacement of embryo-derived cardiac macrophages with age Molawi, Kaaweh Wolf, Yochai Kandalla, Prashanth K. Favret, Jeremy Hagemeyer, Nora Frenzel, Kathrin Pinto, Alexander R. Klapproth, Kay Henri, Sandrine Malissen, Bernard Rodewald, Hans-Reimer Rosenthal, Nadia A. Bajenoff, Marc Prinz, Marco Jung, Steffen Sieweke, Michael H. J Exp Med Brief Definitive Report Cardiac macrophages (cMΦ) are critical for early postnatal heart regeneration and fibrotic repair in the adult heart, but their origins and cellular dynamics during postnatal development have not been well characterized. Tissue macrophages can be derived from embryonic progenitors or from monocytes during inflammation. We report that within the first weeks after birth, the embryo-derived population of resident CX3CR1(+) cMΦ diversifies into MHCII(+) and MHCII(−) cells. Genetic fate mapping demonstrated that cMΦ derived from CX3CR1(+) embryonic progenitors persisted into adulthood but the initially high contribution to resident cMΦ declined after birth. Consistent with this, the early significant proliferation rate of resident cMΦ decreased with age upon diversification into subpopulations. Bone marrow (BM) reconstitution experiments showed monocyte-dependent quantitative replacement of all cMΦ populations. Furthermore, parabiotic mice and BM chimeras of nonirradiated recipient mice revealed a slow but significant donor contribution to cMΦ. Together, our observations indicate that in the heart, embryo-derived cMΦ show declining self-renewal with age and are progressively substituted by monocyte-derived macrophages, even in the absence of inflammation. The Rockefeller University Press 2014-10-20 /pmc/articles/PMC4203946/ /pubmed/25245760 http://dx.doi.org/10.1084/jem.20140639 Text en © 2014 Molawi et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Brief Definitive Report
Molawi, Kaaweh
Wolf, Yochai
Kandalla, Prashanth K.
Favret, Jeremy
Hagemeyer, Nora
Frenzel, Kathrin
Pinto, Alexander R.
Klapproth, Kay
Henri, Sandrine
Malissen, Bernard
Rodewald, Hans-Reimer
Rosenthal, Nadia A.
Bajenoff, Marc
Prinz, Marco
Jung, Steffen
Sieweke, Michael H.
Progressive replacement of embryo-derived cardiac macrophages with age
title Progressive replacement of embryo-derived cardiac macrophages with age
title_full Progressive replacement of embryo-derived cardiac macrophages with age
title_fullStr Progressive replacement of embryo-derived cardiac macrophages with age
title_full_unstemmed Progressive replacement of embryo-derived cardiac macrophages with age
title_short Progressive replacement of embryo-derived cardiac macrophages with age
title_sort progressive replacement of embryo-derived cardiac macrophages with age
topic Brief Definitive Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4203946/
https://www.ncbi.nlm.nih.gov/pubmed/25245760
http://dx.doi.org/10.1084/jem.20140639
work_keys_str_mv AT molawikaaweh progressivereplacementofembryoderivedcardiacmacrophageswithage
AT wolfyochai progressivereplacementofembryoderivedcardiacmacrophageswithage
AT kandallaprashanthk progressivereplacementofembryoderivedcardiacmacrophageswithage
AT favretjeremy progressivereplacementofembryoderivedcardiacmacrophageswithage
AT hagemeyernora progressivereplacementofembryoderivedcardiacmacrophageswithage
AT frenzelkathrin progressivereplacementofembryoderivedcardiacmacrophageswithage
AT pintoalexanderr progressivereplacementofembryoderivedcardiacmacrophageswithage
AT klapprothkay progressivereplacementofembryoderivedcardiacmacrophageswithage
AT henrisandrine progressivereplacementofembryoderivedcardiacmacrophageswithage
AT malissenbernard progressivereplacementofembryoderivedcardiacmacrophageswithage
AT rodewaldhansreimer progressivereplacementofembryoderivedcardiacmacrophageswithage
AT rosenthalnadiaa progressivereplacementofembryoderivedcardiacmacrophageswithage
AT bajenoffmarc progressivereplacementofembryoderivedcardiacmacrophageswithage
AT prinzmarco progressivereplacementofembryoderivedcardiacmacrophageswithage
AT jungsteffen progressivereplacementofembryoderivedcardiacmacrophageswithage
AT siewekemichaelh progressivereplacementofembryoderivedcardiacmacrophageswithage