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A refined model of water and CO(2) membrane diffusion: Effects and contribution of sterols and proteins
Black lipid bilayers, a general model system for biomembranes were studied for diffusion rates of small molecules such as water or CO(2) using advanced analysis techniques and cell free synthesized proteins. We provide evidence that by simple insertion of proteins or sterols the diffusion rates of w...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204046/ https://www.ncbi.nlm.nih.gov/pubmed/25331164 http://dx.doi.org/10.1038/srep06665 |
| Sumario: | Black lipid bilayers, a general model system for biomembranes were studied for diffusion rates of small molecules such as water or CO(2) using advanced analysis techniques and cell free synthesized proteins. We provide evidence that by simple insertion of proteins or sterols the diffusion rates of water or those of CO(2) decrease. Insertion of cell free synthesized water permeable aquaporins restored water diffusion rates as well as insertion of CO(2)-facilitating aquaporins the CO(2) diffusion. Insertion of water or CO(2) impermeable proteins decreased the respective diffusion rates. Therefore, for normal high cellular CO(2) diffusion rates specific aquaporins are mandatory. |
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