A refined model of water and CO(2) membrane diffusion: Effects and contribution of sterols and proteins

Black lipid bilayers, a general model system for biomembranes were studied for diffusion rates of small molecules such as water or CO(2) using advanced analysis techniques and cell free synthesized proteins. We provide evidence that by simple insertion of proteins or sterols the diffusion rates of w...

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Detalles Bibliográficos
Autores principales: Kai, Lei, Kaldenhoff, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204046/
https://www.ncbi.nlm.nih.gov/pubmed/25331164
http://dx.doi.org/10.1038/srep06665
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author Kai, Lei
Kaldenhoff, Ralf
author_facet Kai, Lei
Kaldenhoff, Ralf
author_sort Kai, Lei
collection PubMed
description Black lipid bilayers, a general model system for biomembranes were studied for diffusion rates of small molecules such as water or CO(2) using advanced analysis techniques and cell free synthesized proteins. We provide evidence that by simple insertion of proteins or sterols the diffusion rates of water or those of CO(2) decrease. Insertion of cell free synthesized water permeable aquaporins restored water diffusion rates as well as insertion of CO(2)-facilitating aquaporins the CO(2) diffusion. Insertion of water or CO(2) impermeable proteins decreased the respective diffusion rates. Therefore, for normal high cellular CO(2) diffusion rates specific aquaporins are mandatory.
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spelling pubmed-42040462014-10-24 A refined model of water and CO(2) membrane diffusion: Effects and contribution of sterols and proteins Kai, Lei Kaldenhoff, Ralf Sci Rep Article Black lipid bilayers, a general model system for biomembranes were studied for diffusion rates of small molecules such as water or CO(2) using advanced analysis techniques and cell free synthesized proteins. We provide evidence that by simple insertion of proteins or sterols the diffusion rates of water or those of CO(2) decrease. Insertion of cell free synthesized water permeable aquaporins restored water diffusion rates as well as insertion of CO(2)-facilitating aquaporins the CO(2) diffusion. Insertion of water or CO(2) impermeable proteins decreased the respective diffusion rates. Therefore, for normal high cellular CO(2) diffusion rates specific aquaporins are mandatory. Nature Publishing Group 2014-10-21 /pmc/articles/PMC4204046/ /pubmed/25331164 http://dx.doi.org/10.1038/srep06665 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Article
Kai, Lei
Kaldenhoff, Ralf
A refined model of water and CO(2) membrane diffusion: Effects and contribution of sterols and proteins
title A refined model of water and CO(2) membrane diffusion: Effects and contribution of sterols and proteins
title_full A refined model of water and CO(2) membrane diffusion: Effects and contribution of sterols and proteins
title_fullStr A refined model of water and CO(2) membrane diffusion: Effects and contribution of sterols and proteins
title_full_unstemmed A refined model of water and CO(2) membrane diffusion: Effects and contribution of sterols and proteins
title_short A refined model of water and CO(2) membrane diffusion: Effects and contribution of sterols and proteins
title_sort refined model of water and co(2) membrane diffusion: effects and contribution of sterols and proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204046/
https://www.ncbi.nlm.nih.gov/pubmed/25331164
http://dx.doi.org/10.1038/srep06665
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