Cargando…

Lentiviral Mediating Genetic Engineered Mesenchymal Stem Cells for Releasing IL-27 as a Gene Therapy Approach for Autoimmune Diseases

OBJECTIVE: Autoimmune diseases precede a complex dysregulation of the immune system. T helper17 (Th17) and interleukin (IL)-17 have central roles in initiation of inflammation and subsequent autoimmune diseases. IL-27 significantly controls autoimmune diseases by Th17 and IL-17 suppression. In the p...

Descripción completa

Detalles Bibliográficos
Autores principales: Hajizadeh-Sikaroodi, Shohreh, Hosseini, Ahmad, Fallah, Ali, Estiri, Hajar, Noormohammadi, Zahra, Salehi, Mohammad, Ghaderian, Sayyed Mohammad Hossein, Akhavan Niaki, Haleh, Soleimani, Masoud, Kazemi, Bahram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204184/
https://www.ncbi.nlm.nih.gov/pubmed/24611150
_version_ 1782340516626563072
author Hajizadeh-Sikaroodi, Shohreh
Hosseini, Ahmad
Fallah, Ali
Estiri, Hajar
Noormohammadi, Zahra
Salehi, Mohammad
Ghaderian, Sayyed Mohammad Hossein
Akhavan Niaki, Haleh
Soleimani, Masoud
Kazemi, Bahram
author_facet Hajizadeh-Sikaroodi, Shohreh
Hosseini, Ahmad
Fallah, Ali
Estiri, Hajar
Noormohammadi, Zahra
Salehi, Mohammad
Ghaderian, Sayyed Mohammad Hossein
Akhavan Niaki, Haleh
Soleimani, Masoud
Kazemi, Bahram
author_sort Hajizadeh-Sikaroodi, Shohreh
collection PubMed
description OBJECTIVE: Autoimmune diseases precede a complex dysregulation of the immune system. T helper17 (Th17) and interleukin (IL)-17 have central roles in initiation of inflammation and subsequent autoimmune diseases. IL-27 significantly controls autoimmune diseases by Th17 and IL-17 suppression. In the present study we have created genetic engineered mesenchymal stem cells (MSCs) that mediate with lentiviral vectors to release IL-27 as an adequate vehicle for ex vivo gene therapy in the reduction of inflammation and autoimmune diseases. MATERIALS AND METHODS: In this experimental study, we isolated adipose-derived MSCs (AD-MSCs) from lipoaspirate and subsequently characterized them by differentiation. Two subunits of IL-27 (p28 and EBI3) were cloned in a pCDH-513B-1 lentiviral vector. Expressions of p28 and EBI3 (Epstein-Barr virus induced gene 3) were determined by real time polymerase chain reaction (PCR). MSCs were transduced by a pCDH-CMV-p28-IRES- EBI3-EF-copGFP-Pur lentiviral vector and the bioassay of IL-27 was evaluated by IL-10 expression. RESULTS: Cell differentiation confirmed true isolation of MSCs from lipoaspirate. Restriction enzyme digestion and sequencing verified successful cloning of both p28 and EBI3 in the pCDH-513B-1 lentiviral vector. Real time PCR showed high expressions level of IL-27 and IL-10 as well as accurate activity of IL-27. CONCLUSION: The results showed transduction of functional IL-27 to AD-MSCs by means of a lentiviral vector. The lentiviral vector did not impact MSC characteristics.
format Online
Article
Text
id pubmed-4204184
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Royan Institute
record_format MEDLINE/PubMed
spelling pubmed-42041842014-11-07 Lentiviral Mediating Genetic Engineered Mesenchymal Stem Cells for Releasing IL-27 as a Gene Therapy Approach for Autoimmune Diseases Hajizadeh-Sikaroodi, Shohreh Hosseini, Ahmad Fallah, Ali Estiri, Hajar Noormohammadi, Zahra Salehi, Mohammad Ghaderian, Sayyed Mohammad Hossein Akhavan Niaki, Haleh Soleimani, Masoud Kazemi, Bahram Cell J Original Article OBJECTIVE: Autoimmune diseases precede a complex dysregulation of the immune system. T helper17 (Th17) and interleukin (IL)-17 have central roles in initiation of inflammation and subsequent autoimmune diseases. IL-27 significantly controls autoimmune diseases by Th17 and IL-17 suppression. In the present study we have created genetic engineered mesenchymal stem cells (MSCs) that mediate with lentiviral vectors to release IL-27 as an adequate vehicle for ex vivo gene therapy in the reduction of inflammation and autoimmune diseases. MATERIALS AND METHODS: In this experimental study, we isolated adipose-derived MSCs (AD-MSCs) from lipoaspirate and subsequently characterized them by differentiation. Two subunits of IL-27 (p28 and EBI3) were cloned in a pCDH-513B-1 lentiviral vector. Expressions of p28 and EBI3 (Epstein-Barr virus induced gene 3) were determined by real time polymerase chain reaction (PCR). MSCs were transduced by a pCDH-CMV-p28-IRES- EBI3-EF-copGFP-Pur lentiviral vector and the bioassay of IL-27 was evaluated by IL-10 expression. RESULTS: Cell differentiation confirmed true isolation of MSCs from lipoaspirate. Restriction enzyme digestion and sequencing verified successful cloning of both p28 and EBI3 in the pCDH-513B-1 lentiviral vector. Real time PCR showed high expressions level of IL-27 and IL-10 as well as accurate activity of IL-27. CONCLUSION: The results showed transduction of functional IL-27 to AD-MSCs by means of a lentiviral vector. The lentiviral vector did not impact MSC characteristics. Royan Institute 2014 2014-10-04 /pmc/articles/PMC4204184/ /pubmed/24611150 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Hajizadeh-Sikaroodi, Shohreh
Hosseini, Ahmad
Fallah, Ali
Estiri, Hajar
Noormohammadi, Zahra
Salehi, Mohammad
Ghaderian, Sayyed Mohammad Hossein
Akhavan Niaki, Haleh
Soleimani, Masoud
Kazemi, Bahram
Lentiviral Mediating Genetic Engineered Mesenchymal Stem Cells for Releasing IL-27 as a Gene Therapy Approach for Autoimmune Diseases
title Lentiviral Mediating Genetic Engineered Mesenchymal Stem Cells for Releasing IL-27 as a Gene Therapy Approach for Autoimmune Diseases
title_full Lentiviral Mediating Genetic Engineered Mesenchymal Stem Cells for Releasing IL-27 as a Gene Therapy Approach for Autoimmune Diseases
title_fullStr Lentiviral Mediating Genetic Engineered Mesenchymal Stem Cells for Releasing IL-27 as a Gene Therapy Approach for Autoimmune Diseases
title_full_unstemmed Lentiviral Mediating Genetic Engineered Mesenchymal Stem Cells for Releasing IL-27 as a Gene Therapy Approach for Autoimmune Diseases
title_short Lentiviral Mediating Genetic Engineered Mesenchymal Stem Cells for Releasing IL-27 as a Gene Therapy Approach for Autoimmune Diseases
title_sort lentiviral mediating genetic engineered mesenchymal stem cells for releasing il-27 as a gene therapy approach for autoimmune diseases
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204184/
https://www.ncbi.nlm.nih.gov/pubmed/24611150
work_keys_str_mv AT hajizadehsikaroodishohreh lentiviralmediatinggeneticengineeredmesenchymalstemcellsforreleasingil27asagenetherapyapproachforautoimmunediseases
AT hosseiniahmad lentiviralmediatinggeneticengineeredmesenchymalstemcellsforreleasingil27asagenetherapyapproachforautoimmunediseases
AT fallahali lentiviralmediatinggeneticengineeredmesenchymalstemcellsforreleasingil27asagenetherapyapproachforautoimmunediseases
AT estirihajar lentiviralmediatinggeneticengineeredmesenchymalstemcellsforreleasingil27asagenetherapyapproachforautoimmunediseases
AT noormohammadizahra lentiviralmediatinggeneticengineeredmesenchymalstemcellsforreleasingil27asagenetherapyapproachforautoimmunediseases
AT salehimohammad lentiviralmediatinggeneticengineeredmesenchymalstemcellsforreleasingil27asagenetherapyapproachforautoimmunediseases
AT ghaderiansayyedmohammadhossein lentiviralmediatinggeneticengineeredmesenchymalstemcellsforreleasingil27asagenetherapyapproachforautoimmunediseases
AT akhavanniakihaleh lentiviralmediatinggeneticengineeredmesenchymalstemcellsforreleasingil27asagenetherapyapproachforautoimmunediseases
AT soleimanimasoud lentiviralmediatinggeneticengineeredmesenchymalstemcellsforreleasingil27asagenetherapyapproachforautoimmunediseases
AT kazemibahram lentiviralmediatinggeneticengineeredmesenchymalstemcellsforreleasingil27asagenetherapyapproachforautoimmunediseases