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The Endothelial Permeability Increased by Low Voltage and High Frequency Electroporation

Propose: Standard electroporation and electrochemotherapy caused the endothelial cell permeability and reduction in tumor blood flow. The effects of low voltage and high frequency electroporation on the endothelial cells permeability and viability were expected. Therefore, the propose of present stu...

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Autores principales: Shankayi, Zeinab, Pourmirjafari Firoozabadi, Seyed Mohammad, Zohair Saraf, Hassan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shiraz University of Medical Sciences 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204499/
https://www.ncbi.nlm.nih.gov/pubmed/25505752
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author Shankayi, Zeinab
Pourmirjafari Firoozabadi, Seyed Mohammad
Zohair Saraf, Hassan
author_facet Shankayi, Zeinab
Pourmirjafari Firoozabadi, Seyed Mohammad
Zohair Saraf, Hassan
author_sort Shankayi, Zeinab
collection PubMed
description Propose: Standard electroporation and electrochemotherapy caused the endothelial cell permeability and reduction in tumor blood flow. The effects of low voltage and high frequency electroporation on the endothelial cells permeability and viability were expected. Therefore, the propose of present study was to evaluate the effect of electroporation with bleomycin or alone on viability and permeabilization of human Embryo microvascular endothelial (HUVEC) cell line. Material and Methods: The HUVEC cells were exposed to 4000 electric pulsed with 100µs duration, 50-150 v/cm with increment of 10 v/cm in 4, 5 and 6 kHz frequency (33 experimental groups) and then uptake and viability reduction was measured in each group. Results: The results of this study demonstrated that electric pulses alone reduce the cell viability and with bleomycin significantly increases the toxicity to endothelial cells. Conclusion: Our data indicated that low voltage, high frequency ECT is highly cytotoxic for a HUVEC cell offers a possible effect of antivascular actions of this kind of electrochemotherapy.
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spelling pubmed-42044992014-12-10 The Endothelial Permeability Increased by Low Voltage and High Frequency Electroporation Shankayi, Zeinab Pourmirjafari Firoozabadi, Seyed Mohammad Zohair Saraf, Hassan J Biomed Phys Eng Original Article Propose: Standard electroporation and electrochemotherapy caused the endothelial cell permeability and reduction in tumor blood flow. The effects of low voltage and high frequency electroporation on the endothelial cells permeability and viability were expected. Therefore, the propose of present study was to evaluate the effect of electroporation with bleomycin or alone on viability and permeabilization of human Embryo microvascular endothelial (HUVEC) cell line. Material and Methods: The HUVEC cells were exposed to 4000 electric pulsed with 100µs duration, 50-150 v/cm with increment of 10 v/cm in 4, 5 and 6 kHz frequency (33 experimental groups) and then uptake and viability reduction was measured in each group. Results: The results of this study demonstrated that electric pulses alone reduce the cell viability and with bleomycin significantly increases the toxicity to endothelial cells. Conclusion: Our data indicated that low voltage, high frequency ECT is highly cytotoxic for a HUVEC cell offers a possible effect of antivascular actions of this kind of electrochemotherapy. Shiraz University of Medical Sciences 2013-09-17 /pmc/articles/PMC4204499/ /pubmed/25505752 Text en © 2013: Journal of Biomedical Physics and Engineering This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported License (http://creativecommons.org/licenses/by-nc/3.0/deed.en_US), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Shankayi, Zeinab
Pourmirjafari Firoozabadi, Seyed Mohammad
Zohair Saraf, Hassan
The Endothelial Permeability Increased by Low Voltage and High Frequency Electroporation
title The Endothelial Permeability Increased by Low Voltage and High Frequency Electroporation
title_full The Endothelial Permeability Increased by Low Voltage and High Frequency Electroporation
title_fullStr The Endothelial Permeability Increased by Low Voltage and High Frequency Electroporation
title_full_unstemmed The Endothelial Permeability Increased by Low Voltage and High Frequency Electroporation
title_short The Endothelial Permeability Increased by Low Voltage and High Frequency Electroporation
title_sort endothelial permeability increased by low voltage and high frequency electroporation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204499/
https://www.ncbi.nlm.nih.gov/pubmed/25505752
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