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Expression and purification of recombinant vesicular glutamate transporter VGLUT1 using PC12 cells and High Five insect cells
In synaptic vesicles, the estimated concentration of the excitatory amino acid glutamate is 100-150 mM. It was recently discovered that VGLUT1, previously characterized as an inorganic phosphate transporter (BNPI) with 9-11 predicted transmembrane spanning domains, is capable of transporting glutama...
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| Formato: | Texto |
| Lenguaje: | English |
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Biological Procedures Online
2004
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC420455/ https://www.ncbi.nlm.nih.gov/pubmed/15192755 http://dx.doi.org/10.1251/bpo78 |