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Space-Dependent Formation of Central Pair Microtubules and Their Interactions with Radial Spokes

Cilia and flagella contain nine outer doublet microtubules and a pair of central microtubules. The central pair of microtubules (CP) is important for cilia/flagella beating, as clearly shown by primary ciliary dyskinesia resulting from the loss of the CP. The CP is thought to regulate axonemal dynei...

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Autores principales: Nakazawa, Yuki, Ariyoshi, Tetsuro, Noga, Akira, Kamiya, Ritsu, Hirono, Masafumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204893/
https://www.ncbi.nlm.nih.gov/pubmed/25333940
http://dx.doi.org/10.1371/journal.pone.0110513
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author Nakazawa, Yuki
Ariyoshi, Tetsuro
Noga, Akira
Kamiya, Ritsu
Hirono, Masafumi
author_facet Nakazawa, Yuki
Ariyoshi, Tetsuro
Noga, Akira
Kamiya, Ritsu
Hirono, Masafumi
author_sort Nakazawa, Yuki
collection PubMed
description Cilia and flagella contain nine outer doublet microtubules and a pair of central microtubules. The central pair of microtubules (CP) is important for cilia/flagella beating, as clearly shown by primary ciliary dyskinesia resulting from the loss of the CP. The CP is thought to regulate axonemal dyneins through interaction with radial spokes (RSs). However, the nature of the CP-RS interaction is poorly understood. Here we examine the appearance of CPs in the axonemes of a Chlamydomonas mutant, bld12, which produces axonemes with 8 to 11 outer-doublets. Most of its 8-doublet axonemes lack CPs. However, in the double mutant of bld12 and pf14, a mutant lacking the RS, most 8-doublet axonemes contain the CP. Thus formation of the CP apparently depends on the internal space limited by the outer doublets and RSs. In 10- or 11-doublet axonemes, only 3–5 RSs are attached to the CP and the doublet arrangement is distorted most likely because the RSs attached to the CP pull the outer doublets toward the axonemal center. The CP orientation in the axonemes varies in double mutants formed between bld12 and mutants lacking particular CP projections. The mutant bld12 thus provides the first direct and visual information about the CP-RS interaction, as well as about the mechanism of CP formation.
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spelling pubmed-42048932014-10-27 Space-Dependent Formation of Central Pair Microtubules and Their Interactions with Radial Spokes Nakazawa, Yuki Ariyoshi, Tetsuro Noga, Akira Kamiya, Ritsu Hirono, Masafumi PLoS One Research Article Cilia and flagella contain nine outer doublet microtubules and a pair of central microtubules. The central pair of microtubules (CP) is important for cilia/flagella beating, as clearly shown by primary ciliary dyskinesia resulting from the loss of the CP. The CP is thought to regulate axonemal dyneins through interaction with radial spokes (RSs). However, the nature of the CP-RS interaction is poorly understood. Here we examine the appearance of CPs in the axonemes of a Chlamydomonas mutant, bld12, which produces axonemes with 8 to 11 outer-doublets. Most of its 8-doublet axonemes lack CPs. However, in the double mutant of bld12 and pf14, a mutant lacking the RS, most 8-doublet axonemes contain the CP. Thus formation of the CP apparently depends on the internal space limited by the outer doublets and RSs. In 10- or 11-doublet axonemes, only 3–5 RSs are attached to the CP and the doublet arrangement is distorted most likely because the RSs attached to the CP pull the outer doublets toward the axonemal center. The CP orientation in the axonemes varies in double mutants formed between bld12 and mutants lacking particular CP projections. The mutant bld12 thus provides the first direct and visual information about the CP-RS interaction, as well as about the mechanism of CP formation. Public Library of Science 2014-10-21 /pmc/articles/PMC4204893/ /pubmed/25333940 http://dx.doi.org/10.1371/journal.pone.0110513 Text en © 2014 Nakazawa et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nakazawa, Yuki
Ariyoshi, Tetsuro
Noga, Akira
Kamiya, Ritsu
Hirono, Masafumi
Space-Dependent Formation of Central Pair Microtubules and Their Interactions with Radial Spokes
title Space-Dependent Formation of Central Pair Microtubules and Their Interactions with Radial Spokes
title_full Space-Dependent Formation of Central Pair Microtubules and Their Interactions with Radial Spokes
title_fullStr Space-Dependent Formation of Central Pair Microtubules and Their Interactions with Radial Spokes
title_full_unstemmed Space-Dependent Formation of Central Pair Microtubules and Their Interactions with Radial Spokes
title_short Space-Dependent Formation of Central Pair Microtubules and Their Interactions with Radial Spokes
title_sort space-dependent formation of central pair microtubules and their interactions with radial spokes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204893/
https://www.ncbi.nlm.nih.gov/pubmed/25333940
http://dx.doi.org/10.1371/journal.pone.0110513
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