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Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria

The purpose of this study was to test the suitability of Transgalactosylated oligosaccharides-mupirocin lithium salt (TOS-MUP) and MRS-clindamycin-ciprofloxacin (MRS-CC) agars, along with several other culture media, for selectively enumerating bifidobacteria and lactic acid bacteria (LAB) species c...

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Autores principales: Süle, Judit, Kõrösi, Tímea, Hucker, Attila, Varga, László
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204943/
https://www.ncbi.nlm.nih.gov/pubmed/25477939
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author Süle, Judit
Kõrösi, Tímea
Hucker, Attila
Varga, László
author_facet Süle, Judit
Kõrösi, Tímea
Hucker, Attila
Varga, László
author_sort Süle, Judit
collection PubMed
description The purpose of this study was to test the suitability of Transgalactosylated oligosaccharides-mupirocin lithium salt (TOS-MUP) and MRS-clindamycin-ciprofloxacin (MRS-CC) agars, along with several other culture media, for selectively enumerating bifidobacteria and lactic acid bacteria (LAB) species commonly used to make fermented milks. Pure culture suspensions of a total of 13 dairy bacteria strains, belonging to eight species and five genera, were tested for growth capability under various incubation conditions. TOS-MUP agar was successfully used for the selective enumeration of both Bifidobacterium animalis subsp. lactis BB-12 and B. breve M-16 V. MRS-CC agar showed relatively good selectivity for Lactobacillus acidophilus, however, it also promoted the growth of Lb. casei strains. For this reason, MRS-CC agar can only be used as a selective medium for the enumeration of Lb. acidophilus if Lb. casei is not present in a product at levels similar to or exceeding those of Lb. acidophilus. Unlike bifidobacteria and coccus-shaped LAB, all the lactobacilli strains involved in this work were found to grow well in MRS pH 5.4 agar incubated under anaerobiosis at 37 °C for 72 h. Therefore, this method proved to be particularly suitable for the selective enumeration of Lactobacillus spp.
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spelling pubmed-42049432014-12-04 Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria Süle, Judit Kõrösi, Tímea Hucker, Attila Varga, László Braz J Microbiol Research Paper The purpose of this study was to test the suitability of Transgalactosylated oligosaccharides-mupirocin lithium salt (TOS-MUP) and MRS-clindamycin-ciprofloxacin (MRS-CC) agars, along with several other culture media, for selectively enumerating bifidobacteria and lactic acid bacteria (LAB) species commonly used to make fermented milks. Pure culture suspensions of a total of 13 dairy bacteria strains, belonging to eight species and five genera, were tested for growth capability under various incubation conditions. TOS-MUP agar was successfully used for the selective enumeration of both Bifidobacterium animalis subsp. lactis BB-12 and B. breve M-16 V. MRS-CC agar showed relatively good selectivity for Lactobacillus acidophilus, however, it also promoted the growth of Lb. casei strains. For this reason, MRS-CC agar can only be used as a selective medium for the enumeration of Lb. acidophilus if Lb. casei is not present in a product at levels similar to or exceeding those of Lb. acidophilus. Unlike bifidobacteria and coccus-shaped LAB, all the lactobacilli strains involved in this work were found to grow well in MRS pH 5.4 agar incubated under anaerobiosis at 37 °C for 72 h. Therefore, this method proved to be particularly suitable for the selective enumeration of Lactobacillus spp. Sociedade Brasileira de Microbiologia 2014-10-09 /pmc/articles/PMC4204943/ /pubmed/25477939 Text en Copyright © 2014, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Research Paper
Süle, Judit
Kõrösi, Tímea
Hucker, Attila
Varga, László
Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria
title Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria
title_full Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria
title_fullStr Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria
title_full_unstemmed Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria
title_short Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria
title_sort evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204943/
https://www.ncbi.nlm.nih.gov/pubmed/25477939
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