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Cross-protection between experimental anti-leptospirosis bacterins
We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pig...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204950/ https://www.ncbi.nlm.nih.gov/pubmed/25477946 |
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author | Dib, Cristina Corsi Gonçales, Amane Paldês de Morais, Zenaide Maria de Souza, Gisele Oliveira Miraglia, Fabiana Abreu, Patricia Antonia Estima Vasconcellos, Silvio Arruda |
author_facet | Dib, Cristina Corsi Gonçales, Amane Paldês de Morais, Zenaide Maria de Souza, Gisele Oliveira Miraglia, Fabiana Abreu, Patricia Antonia Estima Vasconcellos, Silvio Arruda |
author_sort | Dib, Cristina Corsi |
collection | PubMed |
description | We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pigs in Brazil. Both were added of aluminum hydroxide as an adjuvant. Experimental bacterins were tested with the hamster potency test in order to assess protection provided against the disease and against the establishment of kidney infection. Controls were polyvalent commercial vaccine produced with Leptospira strains isolated outside Brazil, which included a representative of Pomona serovar, or Sorensen solution added of aluminum hydroxide adjuvant. The challenge was performed with cross-strains of serogroup Pomona tested in accordance with international standards established for the potency test. After 21 days of the challenge, survivors were killed to evaluate the condition of Leptospira renal carrier. Experimental bacterins protected hamsters against homologous and heterologous strains, demonstrating the existence of cross-protection. The commercial vaccine protected the hamsters challenged with both strains, but there was a high proportion of animals diagnosed as renal carriers when the challenge was performed with strain GR6, isolated from pigs in Brazil. |
format | Online Article Text |
id | pubmed-4204950 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-42049502014-12-04 Cross-protection between experimental anti-leptospirosis bacterins Dib, Cristina Corsi Gonçales, Amane Paldês de Morais, Zenaide Maria de Souza, Gisele Oliveira Miraglia, Fabiana Abreu, Patricia Antonia Estima Vasconcellos, Silvio Arruda Braz J Microbiol Research Paper We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pigs in Brazil. Both were added of aluminum hydroxide as an adjuvant. Experimental bacterins were tested with the hamster potency test in order to assess protection provided against the disease and against the establishment of kidney infection. Controls were polyvalent commercial vaccine produced with Leptospira strains isolated outside Brazil, which included a representative of Pomona serovar, or Sorensen solution added of aluminum hydroxide adjuvant. The challenge was performed with cross-strains of serogroup Pomona tested in accordance with international standards established for the potency test. After 21 days of the challenge, survivors were killed to evaluate the condition of Leptospira renal carrier. Experimental bacterins protected hamsters against homologous and heterologous strains, demonstrating the existence of cross-protection. The commercial vaccine protected the hamsters challenged with both strains, but there was a high proportion of animals diagnosed as renal carriers when the challenge was performed with strain GR6, isolated from pigs in Brazil. Sociedade Brasileira de Microbiologia 2014-10-09 /pmc/articles/PMC4204950/ /pubmed/25477946 Text en Copyright © 2014, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC. |
spellingShingle | Research Paper Dib, Cristina Corsi Gonçales, Amane Paldês de Morais, Zenaide Maria de Souza, Gisele Oliveira Miraglia, Fabiana Abreu, Patricia Antonia Estima Vasconcellos, Silvio Arruda Cross-protection between experimental anti-leptospirosis bacterins |
title | Cross-protection between experimental anti-leptospirosis bacterins |
title_full | Cross-protection between experimental anti-leptospirosis bacterins |
title_fullStr | Cross-protection between experimental anti-leptospirosis bacterins |
title_full_unstemmed | Cross-protection between experimental anti-leptospirosis bacterins |
title_short | Cross-protection between experimental anti-leptospirosis bacterins |
title_sort | cross-protection between experimental anti-leptospirosis bacterins |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204950/ https://www.ncbi.nlm.nih.gov/pubmed/25477946 |
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