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Cross-protection between experimental anti-leptospirosis bacterins

We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pig...

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Autores principales: Dib, Cristina Corsi, Gonçales, Amane Paldês, de Morais, Zenaide Maria, de Souza, Gisele Oliveira, Miraglia, Fabiana, Abreu, Patricia Antonia Estima, Vasconcellos, Silvio Arruda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204950/
https://www.ncbi.nlm.nih.gov/pubmed/25477946
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author Dib, Cristina Corsi
Gonçales, Amane Paldês
de Morais, Zenaide Maria
de Souza, Gisele Oliveira
Miraglia, Fabiana
Abreu, Patricia Antonia Estima
Vasconcellos, Silvio Arruda
author_facet Dib, Cristina Corsi
Gonçales, Amane Paldês
de Morais, Zenaide Maria
de Souza, Gisele Oliveira
Miraglia, Fabiana
Abreu, Patricia Antonia Estima
Vasconcellos, Silvio Arruda
author_sort Dib, Cristina Corsi
collection PubMed
description We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pigs in Brazil. Both were added of aluminum hydroxide as an adjuvant. Experimental bacterins were tested with the hamster potency test in order to assess protection provided against the disease and against the establishment of kidney infection. Controls were polyvalent commercial vaccine produced with Leptospira strains isolated outside Brazil, which included a representative of Pomona serovar, or Sorensen solution added of aluminum hydroxide adjuvant. The challenge was performed with cross-strains of serogroup Pomona tested in accordance with international standards established for the potency test. After 21 days of the challenge, survivors were killed to evaluate the condition of Leptospira renal carrier. Experimental bacterins protected hamsters against homologous and heterologous strains, demonstrating the existence of cross-protection. The commercial vaccine protected the hamsters challenged with both strains, but there was a high proportion of animals diagnosed as renal carriers when the challenge was performed with strain GR6, isolated from pigs in Brazil.
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spelling pubmed-42049502014-12-04 Cross-protection between experimental anti-leptospirosis bacterins Dib, Cristina Corsi Gonçales, Amane Paldês de Morais, Zenaide Maria de Souza, Gisele Oliveira Miraglia, Fabiana Abreu, Patricia Antonia Estima Vasconcellos, Silvio Arruda Braz J Microbiol Research Paper We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pigs in Brazil. Both were added of aluminum hydroxide as an adjuvant. Experimental bacterins were tested with the hamster potency test in order to assess protection provided against the disease and against the establishment of kidney infection. Controls were polyvalent commercial vaccine produced with Leptospira strains isolated outside Brazil, which included a representative of Pomona serovar, or Sorensen solution added of aluminum hydroxide adjuvant. The challenge was performed with cross-strains of serogroup Pomona tested in accordance with international standards established for the potency test. After 21 days of the challenge, survivors were killed to evaluate the condition of Leptospira renal carrier. Experimental bacterins protected hamsters against homologous and heterologous strains, demonstrating the existence of cross-protection. The commercial vaccine protected the hamsters challenged with both strains, but there was a high proportion of animals diagnosed as renal carriers when the challenge was performed with strain GR6, isolated from pigs in Brazil. Sociedade Brasileira de Microbiologia 2014-10-09 /pmc/articles/PMC4204950/ /pubmed/25477946 Text en Copyright © 2014, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Research Paper
Dib, Cristina Corsi
Gonçales, Amane Paldês
de Morais, Zenaide Maria
de Souza, Gisele Oliveira
Miraglia, Fabiana
Abreu, Patricia Antonia Estima
Vasconcellos, Silvio Arruda
Cross-protection between experimental anti-leptospirosis bacterins
title Cross-protection between experimental anti-leptospirosis bacterins
title_full Cross-protection between experimental anti-leptospirosis bacterins
title_fullStr Cross-protection between experimental anti-leptospirosis bacterins
title_full_unstemmed Cross-protection between experimental anti-leptospirosis bacterins
title_short Cross-protection between experimental anti-leptospirosis bacterins
title_sort cross-protection between experimental anti-leptospirosis bacterins
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204950/
https://www.ncbi.nlm.nih.gov/pubmed/25477946
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