Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex

We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful...

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Detalles Bibliográficos
Autores principales: Spositto, F.L.E., Campanerut, P.A.Z., Ghiraldi, L.D., Leite, C.Q.F., Hirata, M.H., Hirata, R.D.C., Siqueira, V.L.D., Cardoso, R. Fressatti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2014
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204967/
https://www.ncbi.nlm.nih.gov/pubmed/25477916
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author Spositto, F.L.E.
Campanerut, P.A.Z.
Ghiraldi, L.D.
Leite, C.Q.F.
Hirata, M.H.
Hirata, R.D.C.
Siqueira, V.L.D.
Cardoso, R. Fressatti
author_facet Spositto, F.L.E.
Campanerut, P.A.Z.
Ghiraldi, L.D.
Leite, C.Q.F.
Hirata, M.H.
Hirata, R.D.C.
Siqueira, V.L.D.
Cardoso, R. Fressatti
author_sort Spositto, F.L.E.
collection PubMed
description We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
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spelling pubmed-42049672014-12-04 Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex Spositto, F.L.E. Campanerut, P.A.Z. Ghiraldi, L.D. Leite, C.Q.F. Hirata, M.H. Hirata, R.D.C. Siqueira, V.L.D. Cardoso, R. Fressatti Braz J Microbiol Short Communication We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species. Sociedade Brasileira de Microbiologia 2014-10-09 /pmc/articles/PMC4204967/ /pubmed/25477916 Text en Copyright © 2014, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Short Communication
Spositto, F.L.E.
Campanerut, P.A.Z.
Ghiraldi, L.D.
Leite, C.Q.F.
Hirata, M.H.
Hirata, R.D.C.
Siqueira, V.L.D.
Cardoso, R. Fressatti
Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title_full Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title_fullStr Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title_full_unstemmed Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title_short Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title_sort multiplex-pcr for differentiation of mycobacterium bovis from mycobacterium tuberculosis complex
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204967/
https://www.ncbi.nlm.nih.gov/pubmed/25477916
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