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Enterobacteria identification and detection of diarrheagenic Escherichia coli in a Port Complex
The Port Complex of Maranhão (PCM) is the second largest port complex in Brazil, receiving ships with large volumes of ballast water. To evaluate the microbiological quality of its waters, physicochemical parameters (pH and salinity), the number of coliforms (thermotolerants and totals), and the pre...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204981/ https://www.ncbi.nlm.nih.gov/pubmed/25477930 |
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author | Costa, Clarissa Frota Macatrão Neto, Valério Monteiro Santos, Bruno Rafael de Carvalho Costa, Bruno Rafael Rabelo Azevedo, Alexandre Serra, Josilene Lima Mendes, Hermínio Benítez Rabello Nascimento, Adenilde Ribeiro Mendes, Mariana Bonfim Pinto Kuppinger, Oliver |
author_facet | Costa, Clarissa Frota Macatrão Neto, Valério Monteiro Santos, Bruno Rafael de Carvalho Costa, Bruno Rafael Rabelo Azevedo, Alexandre Serra, Josilene Lima Mendes, Hermínio Benítez Rabello Nascimento, Adenilde Ribeiro Mendes, Mariana Bonfim Pinto Kuppinger, Oliver |
author_sort | Costa, Clarissa Frota Macatrão |
collection | PubMed |
description | The Port Complex of Maranhão (PCM) is the second largest port complex in Brazil, receiving ships with large volumes of ballast water. To evaluate the microbiological quality of its waters, physicochemical parameters (pH and salinity), the number of coliforms (thermotolerants and totals), and the presence of enterobacterias and diarrheagenic Escherichia coli strains were analyzed. In order to identify the presence of E. coli virulence genes target regions of the stx, elt, est, aggR, CVD432, ipaH and eae nucleotide sequences were studied. The presence of totals and thermotolerants coliforms were positive. Analyzing the salinity parameter, a significant increase in total coliforms was observed during the rainy season. We identified the species Escherichia coli, Proteus mirabilis, Citrobacter freundii, Proteus vulgaris, Klebsiella pneumoniae, Klebsiella ozaenae, Morganella morganii, Enterobacter cloacae and Edwardsiella tarda. Out of the 51 E. coli isolated, two were positive for the elt gene and one was positive for the CVD432 sequence, features of enterotoxigenic and enteroaggregative strains, respectively. This study reveals that the PCM is contaminated by enterobacteria and diarrheagenic E.coli thus providing evidence regarding the risk of these bacteria being carried by ships to other countries, and draws attention to the input of fecal bacteria brought by ships in the port waters of Maranhão. |
format | Online Article Text |
id | pubmed-4204981 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-42049812014-12-04 Enterobacteria identification and detection of diarrheagenic Escherichia coli in a Port Complex Costa, Clarissa Frota Macatrão Neto, Valério Monteiro Santos, Bruno Rafael de Carvalho Costa, Bruno Rafael Rabelo Azevedo, Alexandre Serra, Josilene Lima Mendes, Hermínio Benítez Rabello Nascimento, Adenilde Ribeiro Mendes, Mariana Bonfim Pinto Kuppinger, Oliver Braz J Microbiol Research Paper The Port Complex of Maranhão (PCM) is the second largest port complex in Brazil, receiving ships with large volumes of ballast water. To evaluate the microbiological quality of its waters, physicochemical parameters (pH and salinity), the number of coliforms (thermotolerants and totals), and the presence of enterobacterias and diarrheagenic Escherichia coli strains were analyzed. In order to identify the presence of E. coli virulence genes target regions of the stx, elt, est, aggR, CVD432, ipaH and eae nucleotide sequences were studied. The presence of totals and thermotolerants coliforms were positive. Analyzing the salinity parameter, a significant increase in total coliforms was observed during the rainy season. We identified the species Escherichia coli, Proteus mirabilis, Citrobacter freundii, Proteus vulgaris, Klebsiella pneumoniae, Klebsiella ozaenae, Morganella morganii, Enterobacter cloacae and Edwardsiella tarda. Out of the 51 E. coli isolated, two were positive for the elt gene and one was positive for the CVD432 sequence, features of enterotoxigenic and enteroaggregative strains, respectively. This study reveals that the PCM is contaminated by enterobacteria and diarrheagenic E.coli thus providing evidence regarding the risk of these bacteria being carried by ships to other countries, and draws attention to the input of fecal bacteria brought by ships in the port waters of Maranhão. Sociedade Brasileira de Microbiologia 2014-10-09 /pmc/articles/PMC4204981/ /pubmed/25477930 Text en Copyright © 2014, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC. |
spellingShingle | Research Paper Costa, Clarissa Frota Macatrão Neto, Valério Monteiro Santos, Bruno Rafael de Carvalho Costa, Bruno Rafael Rabelo Azevedo, Alexandre Serra, Josilene Lima Mendes, Hermínio Benítez Rabello Nascimento, Adenilde Ribeiro Mendes, Mariana Bonfim Pinto Kuppinger, Oliver Enterobacteria identification and detection of diarrheagenic Escherichia coli in a Port Complex |
title | Enterobacteria identification and detection of diarrheagenic Escherichia coli in a Port Complex |
title_full | Enterobacteria identification and detection of diarrheagenic Escherichia coli in a Port Complex |
title_fullStr | Enterobacteria identification and detection of diarrheagenic Escherichia coli in a Port Complex |
title_full_unstemmed | Enterobacteria identification and detection of diarrheagenic Escherichia coli in a Port Complex |
title_short | Enterobacteria identification and detection of diarrheagenic Escherichia coli in a Port Complex |
title_sort | enterobacteria identification and detection of diarrheagenic escherichia coli in a port complex |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204981/ https://www.ncbi.nlm.nih.gov/pubmed/25477930 |
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