Cargando…

Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa

This study evaluated the impact of different concentrations of glyphosate (Rondup(®)) on planktonic and biofilm growth of P. aeruginosa. Aerobic and anaerobic cultures of P. aeruginosa ATCC(®)15442 inoculated in MHB + glyphosate (0.845 ppm, 1.690 ppm, 8.45 ppm, 16.90 ppm, 84.50 ppm, 169 ppm, 845 ppm...

Descripción completa

Detalles Bibliográficos
Autores principales: Lima, Ilana Schneider, Baumeier, Nicole Carmo, Rosa, Rosimeire Takaki, Campelo, Patrícia Maria Stuelp, Rosa, Edvaldo Antonio Ribeiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204984/
https://www.ncbi.nlm.nih.gov/pubmed/25477933
_version_ 1782340639076122624
author Lima, Ilana Schneider
Baumeier, Nicole Carmo
Rosa, Rosimeire Takaki
Campelo, Patrícia Maria Stuelp
Rosa, Edvaldo Antonio Ribeiro
author_facet Lima, Ilana Schneider
Baumeier, Nicole Carmo
Rosa, Rosimeire Takaki
Campelo, Patrícia Maria Stuelp
Rosa, Edvaldo Antonio Ribeiro
author_sort Lima, Ilana Schneider
collection PubMed
description This study evaluated the impact of different concentrations of glyphosate (Rondup(®)) on planktonic and biofilm growth of P. aeruginosa. Aerobic and anaerobic cultures of P. aeruginosa ATCC(®)15442 inoculated in MHB + glyphosate (0.845 ppm, 1.690 ppm, 8.45 ppm, 16.90 ppm, 84.50 ppm, 169 ppm, 845 ppm, and 1690 ppm) and cultured in normoxia and anoxia, following their OD(560nm) every hour for 24 h. Biofilms of adapted cells were formed in the presence of glyphosate (0.845 to 1690 ppm) in normoxia and anoxia for 36 h. Glyphosate at concentrations higher than 84.5 ppm reduces the cell density of planktonic aerobic cultures (p < 0.05). However, these same concentrations favor the planktonic anaerobic growth (p < 0.05). On the other hand, the herbicide favors a slight growth of biofilms in a concentration-dependent manner up to 84.5 ppm (p > 0.05), and more pronounced over 169 ppm. Anaerobic biofilms have their growth more readily favored (p < 0.05), regardless of concentration. In a concentration-dependent manner, glyphosate interferes with the growth ability of P. aeruginosa ATCC(®)15442.
format Online
Article
Text
id pubmed-4204984
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Sociedade Brasileira de Microbiologia
record_format MEDLINE/PubMed
spelling pubmed-42049842014-12-04 Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa Lima, Ilana Schneider Baumeier, Nicole Carmo Rosa, Rosimeire Takaki Campelo, Patrícia Maria Stuelp Rosa, Edvaldo Antonio Ribeiro Braz J Microbiol Research Paper This study evaluated the impact of different concentrations of glyphosate (Rondup(®)) on planktonic and biofilm growth of P. aeruginosa. Aerobic and anaerobic cultures of P. aeruginosa ATCC(®)15442 inoculated in MHB + glyphosate (0.845 ppm, 1.690 ppm, 8.45 ppm, 16.90 ppm, 84.50 ppm, 169 ppm, 845 ppm, and 1690 ppm) and cultured in normoxia and anoxia, following their OD(560nm) every hour for 24 h. Biofilms of adapted cells were formed in the presence of glyphosate (0.845 to 1690 ppm) in normoxia and anoxia for 36 h. Glyphosate at concentrations higher than 84.5 ppm reduces the cell density of planktonic aerobic cultures (p < 0.05). However, these same concentrations favor the planktonic anaerobic growth (p < 0.05). On the other hand, the herbicide favors a slight growth of biofilms in a concentration-dependent manner up to 84.5 ppm (p > 0.05), and more pronounced over 169 ppm. Anaerobic biofilms have their growth more readily favored (p < 0.05), regardless of concentration. In a concentration-dependent manner, glyphosate interferes with the growth ability of P. aeruginosa ATCC(®)15442. Sociedade Brasileira de Microbiologia 2014-10-09 /pmc/articles/PMC4204984/ /pubmed/25477933 Text en Copyright © 2014, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Research Paper
Lima, Ilana Schneider
Baumeier, Nicole Carmo
Rosa, Rosimeire Takaki
Campelo, Patrícia Maria Stuelp
Rosa, Edvaldo Antonio Ribeiro
Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
title Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
title_full Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
title_fullStr Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
title_full_unstemmed Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
title_short Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
title_sort influence of glyphosate in planktonic and biofilm growth of pseudomonas aeruginosa
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204984/
https://www.ncbi.nlm.nih.gov/pubmed/25477933
work_keys_str_mv AT limailanaschneider influenceofglyphosateinplanktonicandbiofilmgrowthofpseudomonasaeruginosa
AT baumeiernicolecarmo influenceofglyphosateinplanktonicandbiofilmgrowthofpseudomonasaeruginosa
AT rosarosimeiretakaki influenceofglyphosateinplanktonicandbiofilmgrowthofpseudomonasaeruginosa
AT campelopatriciamariastuelp influenceofglyphosateinplanktonicandbiofilmgrowthofpseudomonasaeruginosa
AT rosaedvaldoantonioribeiro influenceofglyphosateinplanktonicandbiofilmgrowthofpseudomonasaeruginosa