A procedure to evaluate the efficiency of surface sterilization methods in culture-independent fungal endophyte studies

Extraneous DNA interferes with PCR studies of endophytic fungi. A procedure was developed with which to evaluate the removal of extraneous DNA. Wheat (Triticum aestivum) leaves were sprayed with Saccharomyces cerevisiae and then subjected to physical and chemical surface treatments. The fungal ITS1...

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Detalles Bibliográficos
Autores principales: Burgdorf, R.J., Laing, M.D., Morris, C.D., Jamal-Ally, S.F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2014
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204985/
https://www.ncbi.nlm.nih.gov/pubmed/25477934

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204985/
https://www.ncbi.nlm.nih.gov/pubmed/25477934

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