Cargando…

Curcumin inhibits EMMPRIN and MMP-9 expression through AMPK-MAPK and PKC signaling in PMA induced macrophages

In coronary arteries, plaque disruption, the major acute clinical manifestations of atherosclerosis, leads to a subsequent cardiac event, such as acute myocardial infarction (AMI) and unstable angina pectoris (UA). Numerous reports have shown that high expression of MMP-9 (matrix metalloproteinase-9...

Descripción completa

Detalles Bibliográficos
Autores principales: Cao, Jiatian, Han, Zhihua, Tian, Lei, Chen, Kan, Fan, Yuqi, Ye, Bozhi, Huang, Weijian, Wang, Changqian, Huang, Zhouqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4205290/
https://www.ncbi.nlm.nih.gov/pubmed/25241044
http://dx.doi.org/10.1186/s12967-014-0266-2
_version_ 1782340674784329728
author Cao, Jiatian
Han, Zhihua
Tian, Lei
Chen, Kan
Fan, Yuqi
Ye, Bozhi
Huang, Weijian
Wang, Changqian
Huang, Zhouqing
author_facet Cao, Jiatian
Han, Zhihua
Tian, Lei
Chen, Kan
Fan, Yuqi
Ye, Bozhi
Huang, Weijian
Wang, Changqian
Huang, Zhouqing
author_sort Cao, Jiatian
collection PubMed
description In coronary arteries, plaque disruption, the major acute clinical manifestations of atherosclerosis, leads to a subsequent cardiac event, such as acute myocardial infarction (AMI) and unstable angina pectoris (UA). Numerous reports have shown that high expression of MMP-9 (matrix metalloproteinase-9), MMP-13 (matrix metalloproteinase-13) and EMMPRIN (extracellular matrix metalloproteinase induce) in monocyte/macrophage results in the plaque progression and destabilization. Curcumin exerts well-known anti-inflammatory and antioxidant effects and probably has a protective role in the atherosclerosis. The purpose of our study was to investigate the molecular mechanisms by which curcumin affects MMP-9, MMP13 and EMMPRIN in PMA (phorbol 12-myristate 13-acetate) induced macrophages. Human monocytic cells (THP-1 cells) were pretreated with curcumin or compound C for 1 h, and then induced by PMA for 48 h. Total RNA and proteins were collected for real-time PCR and Western blot analysis, respectively. In the present study, the exposure to curcumin resulted in attenuated JNK, p38, and ERK activation and decreased expression of MMP-9, MMP-13 and EMMPRIN in PMA induced macrophages. Moreover, we demonstrated that AMPK (AMP-activated protein kinase) and PKC (Protein Kinase C) was activated by PMA during monocyte/macrophage differentiation. Furthermore, curcumin reversed PMA stimulated PKC activation and suppressed the chronic activation of AMPK, which in turn reduced the expression of MMP-9, MMP-13 and EMMPRIN. Therefore, it is suggested that curcumin by inhibiting AMPK-MAPK (mitogen activated protein kinase) and PKC pathway may led to down-regulated EMMPRIN, MMP-9 and MMP-13 expression in PMA-induced THP-1 cells.
format Online
Article
Text
id pubmed-4205290
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-42052902014-10-23 Curcumin inhibits EMMPRIN and MMP-9 expression through AMPK-MAPK and PKC signaling in PMA induced macrophages Cao, Jiatian Han, Zhihua Tian, Lei Chen, Kan Fan, Yuqi Ye, Bozhi Huang, Weijian Wang, Changqian Huang, Zhouqing J Transl Med Research In coronary arteries, plaque disruption, the major acute clinical manifestations of atherosclerosis, leads to a subsequent cardiac event, such as acute myocardial infarction (AMI) and unstable angina pectoris (UA). Numerous reports have shown that high expression of MMP-9 (matrix metalloproteinase-9), MMP-13 (matrix metalloproteinase-13) and EMMPRIN (extracellular matrix metalloproteinase induce) in monocyte/macrophage results in the plaque progression and destabilization. Curcumin exerts well-known anti-inflammatory and antioxidant effects and probably has a protective role in the atherosclerosis. The purpose of our study was to investigate the molecular mechanisms by which curcumin affects MMP-9, MMP13 and EMMPRIN in PMA (phorbol 12-myristate 13-acetate) induced macrophages. Human monocytic cells (THP-1 cells) were pretreated with curcumin or compound C for 1 h, and then induced by PMA for 48 h. Total RNA and proteins were collected for real-time PCR and Western blot analysis, respectively. In the present study, the exposure to curcumin resulted in attenuated JNK, p38, and ERK activation and decreased expression of MMP-9, MMP-13 and EMMPRIN in PMA induced macrophages. Moreover, we demonstrated that AMPK (AMP-activated protein kinase) and PKC (Protein Kinase C) was activated by PMA during monocyte/macrophage differentiation. Furthermore, curcumin reversed PMA stimulated PKC activation and suppressed the chronic activation of AMPK, which in turn reduced the expression of MMP-9, MMP-13 and EMMPRIN. Therefore, it is suggested that curcumin by inhibiting AMPK-MAPK (mitogen activated protein kinase) and PKC pathway may led to down-regulated EMMPRIN, MMP-9 and MMP-13 expression in PMA-induced THP-1 cells. BioMed Central 2014-09-21 /pmc/articles/PMC4205290/ /pubmed/25241044 http://dx.doi.org/10.1186/s12967-014-0266-2 Text en © Cao et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Cao, Jiatian
Han, Zhihua
Tian, Lei
Chen, Kan
Fan, Yuqi
Ye, Bozhi
Huang, Weijian
Wang, Changqian
Huang, Zhouqing
Curcumin inhibits EMMPRIN and MMP-9 expression through AMPK-MAPK and PKC signaling in PMA induced macrophages
title Curcumin inhibits EMMPRIN and MMP-9 expression through AMPK-MAPK and PKC signaling in PMA induced macrophages
title_full Curcumin inhibits EMMPRIN and MMP-9 expression through AMPK-MAPK and PKC signaling in PMA induced macrophages
title_fullStr Curcumin inhibits EMMPRIN and MMP-9 expression through AMPK-MAPK and PKC signaling in PMA induced macrophages
title_full_unstemmed Curcumin inhibits EMMPRIN and MMP-9 expression through AMPK-MAPK and PKC signaling in PMA induced macrophages
title_short Curcumin inhibits EMMPRIN and MMP-9 expression through AMPK-MAPK and PKC signaling in PMA induced macrophages
title_sort curcumin inhibits emmprin and mmp-9 expression through ampk-mapk and pkc signaling in pma induced macrophages
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4205290/
https://www.ncbi.nlm.nih.gov/pubmed/25241044
http://dx.doi.org/10.1186/s12967-014-0266-2
work_keys_str_mv AT caojiatian curcumininhibitsemmprinandmmp9expressionthroughampkmapkandpkcsignalinginpmainducedmacrophages
AT hanzhihua curcumininhibitsemmprinandmmp9expressionthroughampkmapkandpkcsignalinginpmainducedmacrophages
AT tianlei curcumininhibitsemmprinandmmp9expressionthroughampkmapkandpkcsignalinginpmainducedmacrophages
AT chenkan curcumininhibitsemmprinandmmp9expressionthroughampkmapkandpkcsignalinginpmainducedmacrophages
AT fanyuqi curcumininhibitsemmprinandmmp9expressionthroughampkmapkandpkcsignalinginpmainducedmacrophages
AT yebozhi curcumininhibitsemmprinandmmp9expressionthroughampkmapkandpkcsignalinginpmainducedmacrophages
AT huangweijian curcumininhibitsemmprinandmmp9expressionthroughampkmapkandpkcsignalinginpmainducedmacrophages
AT wangchangqian curcumininhibitsemmprinandmmp9expressionthroughampkmapkandpkcsignalinginpmainducedmacrophages
AT huangzhouqing curcumininhibitsemmprinandmmp9expressionthroughampkmapkandpkcsignalinginpmainducedmacrophages