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Increase of TRPV1-Immunoreactivity in Dorsal Root Ganglia Neurons Innervating the Femur in a Rat Model of Osteoporosis

PURPOSE: Transient receptor potential vanilloid 1 (TRPV1) is a ligand-gated nonselective cation channel, which can be activated by capsaicin and other noxious stimuli. Recently, an association between bone pain and TRPV1 has been reported. However, the influence of osteoporosis on TRPV1 in the senso...

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Autores principales: Yoshino, Kensuke, Suzuki, Miyako, Kawarai, Yuya, Sakuma, Yoshihiro, Inoue, Gen, Orita, Sumihisa, Yamauchi, Kazuyo, Aoki, Yasuchika, Ishikawa, Tetsuhiro, Miyagi, Masayuki, Kamoda, Hiroto, Kubota, Gou, Oikawa, Yasuhiro, Inage, Kazuhide, Sainoh, Takeshi, Sato, Jun, Nakamura, Junichi, Toyone, Tomoaki, Takahashi, Kazuhisa, Ohtori, Seiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Yonsei University College of Medicine 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4205700/
https://www.ncbi.nlm.nih.gov/pubmed/25323897
http://dx.doi.org/10.3349/ymj.2014.55.6.1600
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author Yoshino, Kensuke
Suzuki, Miyako
Kawarai, Yuya
Sakuma, Yoshihiro
Inoue, Gen
Orita, Sumihisa
Yamauchi, Kazuyo
Aoki, Yasuchika
Ishikawa, Tetsuhiro
Miyagi, Masayuki
Kamoda, Hiroto
Kubota, Gou
Oikawa, Yasuhiro
Inage, Kazuhide
Sainoh, Takeshi
Sato, Jun
Nakamura, Junichi
Toyone, Tomoaki
Takahashi, Kazuhisa
Ohtori, Seiji
author_facet Yoshino, Kensuke
Suzuki, Miyako
Kawarai, Yuya
Sakuma, Yoshihiro
Inoue, Gen
Orita, Sumihisa
Yamauchi, Kazuyo
Aoki, Yasuchika
Ishikawa, Tetsuhiro
Miyagi, Masayuki
Kamoda, Hiroto
Kubota, Gou
Oikawa, Yasuhiro
Inage, Kazuhide
Sainoh, Takeshi
Sato, Jun
Nakamura, Junichi
Toyone, Tomoaki
Takahashi, Kazuhisa
Ohtori, Seiji
author_sort Yoshino, Kensuke
collection PubMed
description PURPOSE: Transient receptor potential vanilloid 1 (TRPV1) is a ligand-gated nonselective cation channel, which can be activated by capsaicin and other noxious stimuli. Recently, an association between bone pain and TRPV1 has been reported. However, the influence of osteoporosis on TRPV1 in the sensory system innervating the femur has not been reported. MATERIALS AND METHODS: TRPV1-immunoreactive (ir) in dorsal root ganglia (DRG) neurons labeled with neurotracer [Fluoro-Gold (FG)] innervating the femurs of Sprague Dawley rats were examined in control, sham, and ovariectomized (OVX) rats. We evaluated osteoporosis in the femurs and compared the proportion of TRPV1-ir DRG neurons innervating femur between the 3 groups of rats. RESULTS: OVX rats showed osteoporotic cancellous bone in the femur. FG labeled neurons were distributed from L1 to L6 DRG, but there was no significant difference in the proportion of labeled neurons between the 3 groups (p>0.05). The proportions of FG labeled TRPV1-ir DRG neurons were 1.7%, 1.7%, and 2.8% of DRG neurons innervating the femur, in control, sham-operated, and OVX rats, respectively. The proportion of TRPV1-ir neurons in DRG innervating the femur in OVX rats was significantly higher than that in control and sham-operated rats (p<0.05). CONCLUSION: Under physiological conditions, DRG neurons innervating femurs in rats contain TRPV1. Osteoporosis increases the numbers of TRPV1-ir neurons in DRG innervating osteoporotic femurs in rats. These findings suggest that TRPV1 may have a role in sensory perception of osteoporotic femurs.
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spelling pubmed-42057002014-11-01 Increase of TRPV1-Immunoreactivity in Dorsal Root Ganglia Neurons Innervating the Femur in a Rat Model of Osteoporosis Yoshino, Kensuke Suzuki, Miyako Kawarai, Yuya Sakuma, Yoshihiro Inoue, Gen Orita, Sumihisa Yamauchi, Kazuyo Aoki, Yasuchika Ishikawa, Tetsuhiro Miyagi, Masayuki Kamoda, Hiroto Kubota, Gou Oikawa, Yasuhiro Inage, Kazuhide Sainoh, Takeshi Sato, Jun Nakamura, Junichi Toyone, Tomoaki Takahashi, Kazuhisa Ohtori, Seiji Yonsei Med J Original Article PURPOSE: Transient receptor potential vanilloid 1 (TRPV1) is a ligand-gated nonselective cation channel, which can be activated by capsaicin and other noxious stimuli. Recently, an association between bone pain and TRPV1 has been reported. However, the influence of osteoporosis on TRPV1 in the sensory system innervating the femur has not been reported. MATERIALS AND METHODS: TRPV1-immunoreactive (ir) in dorsal root ganglia (DRG) neurons labeled with neurotracer [Fluoro-Gold (FG)] innervating the femurs of Sprague Dawley rats were examined in control, sham, and ovariectomized (OVX) rats. We evaluated osteoporosis in the femurs and compared the proportion of TRPV1-ir DRG neurons innervating femur between the 3 groups of rats. RESULTS: OVX rats showed osteoporotic cancellous bone in the femur. FG labeled neurons were distributed from L1 to L6 DRG, but there was no significant difference in the proportion of labeled neurons between the 3 groups (p>0.05). The proportions of FG labeled TRPV1-ir DRG neurons were 1.7%, 1.7%, and 2.8% of DRG neurons innervating the femur, in control, sham-operated, and OVX rats, respectively. The proportion of TRPV1-ir neurons in DRG innervating the femur in OVX rats was significantly higher than that in control and sham-operated rats (p<0.05). CONCLUSION: Under physiological conditions, DRG neurons innervating femurs in rats contain TRPV1. Osteoporosis increases the numbers of TRPV1-ir neurons in DRG innervating osteoporotic femurs in rats. These findings suggest that TRPV1 may have a role in sensory perception of osteoporotic femurs. Yonsei University College of Medicine 2014-11-01 2014-10-08 /pmc/articles/PMC4205700/ /pubmed/25323897 http://dx.doi.org/10.3349/ymj.2014.55.6.1600 Text en © Copyright: Yonsei University College of Medicine 2014 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Yoshino, Kensuke
Suzuki, Miyako
Kawarai, Yuya
Sakuma, Yoshihiro
Inoue, Gen
Orita, Sumihisa
Yamauchi, Kazuyo
Aoki, Yasuchika
Ishikawa, Tetsuhiro
Miyagi, Masayuki
Kamoda, Hiroto
Kubota, Gou
Oikawa, Yasuhiro
Inage, Kazuhide
Sainoh, Takeshi
Sato, Jun
Nakamura, Junichi
Toyone, Tomoaki
Takahashi, Kazuhisa
Ohtori, Seiji
Increase of TRPV1-Immunoreactivity in Dorsal Root Ganglia Neurons Innervating the Femur in a Rat Model of Osteoporosis
title Increase of TRPV1-Immunoreactivity in Dorsal Root Ganglia Neurons Innervating the Femur in a Rat Model of Osteoporosis
title_full Increase of TRPV1-Immunoreactivity in Dorsal Root Ganglia Neurons Innervating the Femur in a Rat Model of Osteoporosis
title_fullStr Increase of TRPV1-Immunoreactivity in Dorsal Root Ganglia Neurons Innervating the Femur in a Rat Model of Osteoporosis
title_full_unstemmed Increase of TRPV1-Immunoreactivity in Dorsal Root Ganglia Neurons Innervating the Femur in a Rat Model of Osteoporosis
title_short Increase of TRPV1-Immunoreactivity in Dorsal Root Ganglia Neurons Innervating the Femur in a Rat Model of Osteoporosis
title_sort increase of trpv1-immunoreactivity in dorsal root ganglia neurons innervating the femur in a rat model of osteoporosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4205700/
https://www.ncbi.nlm.nih.gov/pubmed/25323897
http://dx.doi.org/10.3349/ymj.2014.55.6.1600
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