Cargando…

Lab-on-a-brain: Implantable micro-optical fluidic devices for neural cell analysis in vivo

The high-resolution imaging of neural cells in vivo has brought about great progress in neuroscience research. Here, we report a novel experimental platform, where the intact brain of a living mouse can be studied with the aid of a surgically implanted micro-optical fluidic device; acting as an inte...

Descripción completa

Detalles Bibliográficos
Autores principales: Takehara, Hiroaki, Nagaoka, Akira, Noguchi, Jun, Akagi, Takanori, Kasai, Haruo, Ichiki, Takanori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4205880/
https://www.ncbi.nlm.nih.gov/pubmed/25335545
http://dx.doi.org/10.1038/srep06721
_version_ 1782340733145972736
author Takehara, Hiroaki
Nagaoka, Akira
Noguchi, Jun
Akagi, Takanori
Kasai, Haruo
Ichiki, Takanori
author_facet Takehara, Hiroaki
Nagaoka, Akira
Noguchi, Jun
Akagi, Takanori
Kasai, Haruo
Ichiki, Takanori
author_sort Takehara, Hiroaki
collection PubMed
description The high-resolution imaging of neural cells in vivo has brought about great progress in neuroscience research. Here, we report a novel experimental platform, where the intact brain of a living mouse can be studied with the aid of a surgically implanted micro-optical fluidic device; acting as an interface between neurons and the outer world. The newly developed device provides the functions required for the long-term and high-resolution observation of the fine structures of neurons by two-photon laser scanning microscopy and the microfluidic delivery of chemicals or drugs directly into the brain. A proof-of-concept experiment of single-synapse stimulation by two-photon uncaging of caged glutamate and observation of dendritic spine shrinkage over subsequent days demonstrated a promising use for the present technology.
format Online
Article
Text
id pubmed-4205880
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-42058802014-10-24 Lab-on-a-brain: Implantable micro-optical fluidic devices for neural cell analysis in vivo Takehara, Hiroaki Nagaoka, Akira Noguchi, Jun Akagi, Takanori Kasai, Haruo Ichiki, Takanori Sci Rep Article The high-resolution imaging of neural cells in vivo has brought about great progress in neuroscience research. Here, we report a novel experimental platform, where the intact brain of a living mouse can be studied with the aid of a surgically implanted micro-optical fluidic device; acting as an interface between neurons and the outer world. The newly developed device provides the functions required for the long-term and high-resolution observation of the fine structures of neurons by two-photon laser scanning microscopy and the microfluidic delivery of chemicals or drugs directly into the brain. A proof-of-concept experiment of single-synapse stimulation by two-photon uncaging of caged glutamate and observation of dendritic spine shrinkage over subsequent days demonstrated a promising use for the present technology. Nature Publishing Group 2014-10-22 /pmc/articles/PMC4205880/ /pubmed/25335545 http://dx.doi.org/10.1038/srep06721 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Takehara, Hiroaki
Nagaoka, Akira
Noguchi, Jun
Akagi, Takanori
Kasai, Haruo
Ichiki, Takanori
Lab-on-a-brain: Implantable micro-optical fluidic devices for neural cell analysis in vivo
title Lab-on-a-brain: Implantable micro-optical fluidic devices for neural cell analysis in vivo
title_full Lab-on-a-brain: Implantable micro-optical fluidic devices for neural cell analysis in vivo
title_fullStr Lab-on-a-brain: Implantable micro-optical fluidic devices for neural cell analysis in vivo
title_full_unstemmed Lab-on-a-brain: Implantable micro-optical fluidic devices for neural cell analysis in vivo
title_short Lab-on-a-brain: Implantable micro-optical fluidic devices for neural cell analysis in vivo
title_sort lab-on-a-brain: implantable micro-optical fluidic devices for neural cell analysis in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4205880/
https://www.ncbi.nlm.nih.gov/pubmed/25335545
http://dx.doi.org/10.1038/srep06721
work_keys_str_mv AT takeharahiroaki labonabrainimplantablemicroopticalfluidicdevicesforneuralcellanalysisinvivo
AT nagaokaakira labonabrainimplantablemicroopticalfluidicdevicesforneuralcellanalysisinvivo
AT noguchijun labonabrainimplantablemicroopticalfluidicdevicesforneuralcellanalysisinvivo
AT akagitakanori labonabrainimplantablemicroopticalfluidicdevicesforneuralcellanalysisinvivo
AT kasaiharuo labonabrainimplantablemicroopticalfluidicdevicesforneuralcellanalysisinvivo
AT ichikitakanori labonabrainimplantablemicroopticalfluidicdevicesforneuralcellanalysisinvivo