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Irisin Promotes Human Umbilical Vein Endothelial Cell Proliferation through the ERK Signaling Pathway and Partly Suppresses High Glucose-Induced Apoptosis

Irisin is a newly discovered myokine that links exercise with metabolic homeostasis. It is involved in modest weight loss and improves glucose intolerance. However, the direct effects and mechanisms of irisin on vascular endothelial cells (ECs) are not fully understood. In the current study, we demo...

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Autores principales: Song, Haibo, Wu, Fei, Zhang, Yuan, Zhang, Yuzhu, Wang, Fang, Jiang, Miao, Wang, Zhongde, Zhang, Mingxiang, Li, Shiwu, Yang, Lijun, Wang, Xing Li, Cui, Taixing, Tang, Dongqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206299/
https://www.ncbi.nlm.nih.gov/pubmed/25338001
http://dx.doi.org/10.1371/journal.pone.0110273
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author Song, Haibo
Wu, Fei
Zhang, Yuan
Zhang, Yuzhu
Wang, Fang
Jiang, Miao
Wang, Zhongde
Zhang, Mingxiang
Li, Shiwu
Yang, Lijun
Wang, Xing Li
Cui, Taixing
Tang, Dongqi
author_facet Song, Haibo
Wu, Fei
Zhang, Yuan
Zhang, Yuzhu
Wang, Fang
Jiang, Miao
Wang, Zhongde
Zhang, Mingxiang
Li, Shiwu
Yang, Lijun
Wang, Xing Li
Cui, Taixing
Tang, Dongqi
author_sort Song, Haibo
collection PubMed
description Irisin is a newly discovered myokine that links exercise with metabolic homeostasis. It is involved in modest weight loss and improves glucose intolerance. However, the direct effects and mechanisms of irisin on vascular endothelial cells (ECs) are not fully understood. In the current study, we demonstrated that irisin promoted Human Umbilical Vein Endothelial Cell (HUVEC) proliferation. It was further demonstrated that this pro-proliferation effect was mediated by irisin-induced activation of extracellular signal–related kinase (ERK) signaling pathways. Inhibition of ERK signaling with U0126 decreased the pro-proliferation effect of irisin on HUVECs. It was also demonstrated that irisin reduced high glucose-induced apoptosis by up-regulating Bcl-2 expression and down-regulating Bax, Caspase-9 and Caspase-3 expression. In summary, these results suggested that irisin plays a novel role in sustaining endothelial homeostasis by promoting HUVEC proliferation via the ERK signaling pathway and protects the cell from high glucose-induced apoptosis by regulating Bcl-2,Bax and Caspase expression.
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spelling pubmed-42062992014-10-27 Irisin Promotes Human Umbilical Vein Endothelial Cell Proliferation through the ERK Signaling Pathway and Partly Suppresses High Glucose-Induced Apoptosis Song, Haibo Wu, Fei Zhang, Yuan Zhang, Yuzhu Wang, Fang Jiang, Miao Wang, Zhongde Zhang, Mingxiang Li, Shiwu Yang, Lijun Wang, Xing Li Cui, Taixing Tang, Dongqi PLoS One Research Article Irisin is a newly discovered myokine that links exercise with metabolic homeostasis. It is involved in modest weight loss and improves glucose intolerance. However, the direct effects and mechanisms of irisin on vascular endothelial cells (ECs) are not fully understood. In the current study, we demonstrated that irisin promoted Human Umbilical Vein Endothelial Cell (HUVEC) proliferation. It was further demonstrated that this pro-proliferation effect was mediated by irisin-induced activation of extracellular signal–related kinase (ERK) signaling pathways. Inhibition of ERK signaling with U0126 decreased the pro-proliferation effect of irisin on HUVECs. It was also demonstrated that irisin reduced high glucose-induced apoptosis by up-regulating Bcl-2 expression and down-regulating Bax, Caspase-9 and Caspase-3 expression. In summary, these results suggested that irisin plays a novel role in sustaining endothelial homeostasis by promoting HUVEC proliferation via the ERK signaling pathway and protects the cell from high glucose-induced apoptosis by regulating Bcl-2,Bax and Caspase expression. Public Library of Science 2014-10-22 /pmc/articles/PMC4206299/ /pubmed/25338001 http://dx.doi.org/10.1371/journal.pone.0110273 Text en © 2014 Song et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Song, Haibo
Wu, Fei
Zhang, Yuan
Zhang, Yuzhu
Wang, Fang
Jiang, Miao
Wang, Zhongde
Zhang, Mingxiang
Li, Shiwu
Yang, Lijun
Wang, Xing Li
Cui, Taixing
Tang, Dongqi
Irisin Promotes Human Umbilical Vein Endothelial Cell Proliferation through the ERK Signaling Pathway and Partly Suppresses High Glucose-Induced Apoptosis
title Irisin Promotes Human Umbilical Vein Endothelial Cell Proliferation through the ERK Signaling Pathway and Partly Suppresses High Glucose-Induced Apoptosis
title_full Irisin Promotes Human Umbilical Vein Endothelial Cell Proliferation through the ERK Signaling Pathway and Partly Suppresses High Glucose-Induced Apoptosis
title_fullStr Irisin Promotes Human Umbilical Vein Endothelial Cell Proliferation through the ERK Signaling Pathway and Partly Suppresses High Glucose-Induced Apoptosis
title_full_unstemmed Irisin Promotes Human Umbilical Vein Endothelial Cell Proliferation through the ERK Signaling Pathway and Partly Suppresses High Glucose-Induced Apoptosis
title_short Irisin Promotes Human Umbilical Vein Endothelial Cell Proliferation through the ERK Signaling Pathway and Partly Suppresses High Glucose-Induced Apoptosis
title_sort irisin promotes human umbilical vein endothelial cell proliferation through the erk signaling pathway and partly suppresses high glucose-induced apoptosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206299/
https://www.ncbi.nlm.nih.gov/pubmed/25338001
http://dx.doi.org/10.1371/journal.pone.0110273
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