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Cytotoxicity Evaluation of Iranian Propolis and Calcium Hydroxide on Dental Pulp Fibroblasts

Background and aims. Since intracanal medicaments can affect the cell viability in periapical tissues, the aim of this study was to evaluate the effect of calcium hydroxide and propolis on pulp fibroblasts. Materials and methods. Two healthy third molars were used as a source to obtain fibroblasts....

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Autores principales: Zare Jahromi, Maryam, Ranjbarian, Parisa, Shiravi, Samaneh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206753/
https://www.ncbi.nlm.nih.gov/pubmed/25346830
http://dx.doi.org/10.5681/joddd.2014.024
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author Zare Jahromi, Maryam
Ranjbarian, Parisa
Shiravi, Samaneh
author_facet Zare Jahromi, Maryam
Ranjbarian, Parisa
Shiravi, Samaneh
author_sort Zare Jahromi, Maryam
collection PubMed
description Background and aims. Since intracanal medicaments can affect the cell viability in periapical tissues, the aim of this study was to evaluate the effect of calcium hydroxide and propolis on pulp fibroblasts. Materials and methods. Two healthy third molars were used as a source to obtain fibroblasts. The fibroblasts were cultured and subjected to 1 mg/mL of propolis and calcium hydroxide. This experiment was performed in six replicates and cell viability was evaluated with MTT assay. Statistical analysis was performed by t-test. Results. Comparison of cell viability with the use of 1 mg/mL of calcium hydroxide and propolis showed that cells subjected to propolis were more viable when compared to calcium hydroxide (P < 0.05). Conclusion. In this study, calcium hydroxide reduced fibroblast viability, significantly more than Iranian propolis. Other properties should be evaluated before Iranian propolis could be indicated for use as intracanal medicament.
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spelling pubmed-42067532014-10-24 Cytotoxicity Evaluation of Iranian Propolis and Calcium Hydroxide on Dental Pulp Fibroblasts Zare Jahromi, Maryam Ranjbarian, Parisa Shiravi, Samaneh J Dent Res Dent Clin Dent Prospects Original Article Background and aims. Since intracanal medicaments can affect the cell viability in periapical tissues, the aim of this study was to evaluate the effect of calcium hydroxide and propolis on pulp fibroblasts. Materials and methods. Two healthy third molars were used as a source to obtain fibroblasts. The fibroblasts were cultured and subjected to 1 mg/mL of propolis and calcium hydroxide. This experiment was performed in six replicates and cell viability was evaluated with MTT assay. Statistical analysis was performed by t-test. Results. Comparison of cell viability with the use of 1 mg/mL of calcium hydroxide and propolis showed that cells subjected to propolis were more viable when compared to calcium hydroxide (P < 0.05). Conclusion. In this study, calcium hydroxide reduced fibroblast viability, significantly more than Iranian propolis. Other properties should be evaluated before Iranian propolis could be indicated for use as intracanal medicament. Tabriz University of Medical Sciences 2014 2014-09-17 /pmc/articles/PMC4206753/ /pubmed/25346830 http://dx.doi.org/10.5681/joddd.2014.024 Text en © 2014 The Authors; Tabriz University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 License(http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zare Jahromi, Maryam
Ranjbarian, Parisa
Shiravi, Samaneh
Cytotoxicity Evaluation of Iranian Propolis and Calcium Hydroxide on Dental Pulp Fibroblasts
title Cytotoxicity Evaluation of Iranian Propolis and Calcium Hydroxide on Dental Pulp Fibroblasts
title_full Cytotoxicity Evaluation of Iranian Propolis and Calcium Hydroxide on Dental Pulp Fibroblasts
title_fullStr Cytotoxicity Evaluation of Iranian Propolis and Calcium Hydroxide on Dental Pulp Fibroblasts
title_full_unstemmed Cytotoxicity Evaluation of Iranian Propolis and Calcium Hydroxide on Dental Pulp Fibroblasts
title_short Cytotoxicity Evaluation of Iranian Propolis and Calcium Hydroxide on Dental Pulp Fibroblasts
title_sort cytotoxicity evaluation of iranian propolis and calcium hydroxide on dental pulp fibroblasts
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206753/
https://www.ncbi.nlm.nih.gov/pubmed/25346830
http://dx.doi.org/10.5681/joddd.2014.024
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