N-Methyl-4-hydrazino-7-nitrobenzofurazan: a fluorogenic substrate for peroxidase-like DNAzyme, and its potential application

Characterization and optimization studies of N-methyl-4-hydrazino-7-nitrobenzofurazan (MNBDH) as a new fluorogenic substrate in the peroxidation reaction catalyzed by DNAzyme are reported. The effects of pH, H(2)O(2) concentration, metal-cation type, and the concentration and type of surfactant on t...

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Detalles Bibliográficos
Autores principales: Kosman, Joanna, Wu, Yu-Tang, Gluszynska, Agata, Juskowiak, Bernard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Paper in Forefront
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206775/
https://www.ncbi.nlm.nih.gov/pubmed/25213215
http://dx.doi.org/10.1007/s00216-014-8119-7
Descripción
Sumario:Characterization and optimization studies of N-methyl-4-hydrazino-7-nitrobenzofurazan (MNBDH) as a new fluorogenic substrate in the peroxidation reaction catalyzed by DNAzyme are reported. The effects of pH, H(2)O(2) concentration, metal-cation type, and the concentration and type of surfactant on the fluorescence intensity were investigated. The optimized reaction was subsequently used for the development of an assay for DNA detection based on a molecular-beacon probe. The use of a fluorogenic substrate enabled the detection of a single-stranded DNA target with a 1 nmol L(−1) detection limit. [Figure: see text]

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