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Laser spectrometry for multi-elemental imaging of biological tissues

An increasing interest has arisen in research focused on metallic and organic ions that play crucial roles in both physiological and pathological metabolic processes. Current methods for the observation of trace elements in biological tissues at microscopic spatial resolution often require equipment...

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Autores principales: Sancey, L., Motto-Ros, V., Busser, B., Kotb, S., Benoit, J. M., Piednoir, A., Lux, F., Tillement, O., Panczer, G., Yu, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206947/
https://www.ncbi.nlm.nih.gov/pubmed/25338518
http://dx.doi.org/10.1038/srep06065
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author Sancey, L.
Motto-Ros, V.
Busser, B.
Kotb, S.
Benoit, J. M.
Piednoir, A.
Lux, F.
Tillement, O.
Panczer, G.
Yu, J.
author_facet Sancey, L.
Motto-Ros, V.
Busser, B.
Kotb, S.
Benoit, J. M.
Piednoir, A.
Lux, F.
Tillement, O.
Panczer, G.
Yu, J.
author_sort Sancey, L.
collection PubMed
description An increasing interest has arisen in research focused on metallic and organic ions that play crucial roles in both physiological and pathological metabolic processes. Current methods for the observation of trace elements in biological tissues at microscopic spatial resolution often require equipment with high complexity. We demonstrate a novel approach with an all-optical design and multi-elemental scanning imaging, which is unique among methods of elemental detection because of its full compatibility with standard optical microscopy. This approach is based on laser-induced breakdown spectroscopy (LIBS), which allows the elements in a tissue sample to be directly detected and quantified under atmospheric pressure. We successfully applied this method to murine kidneys with 10 µm resolution and a ppm-level detection limit to analyze the renal clearance of nanoparticles. These results offer new insight into the use of laser spectrometry in biomedical applications in the field of label-free elemental mapping of biological tissues.
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spelling pubmed-42069472014-10-24 Laser spectrometry for multi-elemental imaging of biological tissues Sancey, L. Motto-Ros, V. Busser, B. Kotb, S. Benoit, J. M. Piednoir, A. Lux, F. Tillement, O. Panczer, G. Yu, J. Sci Rep Article An increasing interest has arisen in research focused on metallic and organic ions that play crucial roles in both physiological and pathological metabolic processes. Current methods for the observation of trace elements in biological tissues at microscopic spatial resolution often require equipment with high complexity. We demonstrate a novel approach with an all-optical design and multi-elemental scanning imaging, which is unique among methods of elemental detection because of its full compatibility with standard optical microscopy. This approach is based on laser-induced breakdown spectroscopy (LIBS), which allows the elements in a tissue sample to be directly detected and quantified under atmospheric pressure. We successfully applied this method to murine kidneys with 10 µm resolution and a ppm-level detection limit to analyze the renal clearance of nanoparticles. These results offer new insight into the use of laser spectrometry in biomedical applications in the field of label-free elemental mapping of biological tissues. Nature Publishing Group 2014-08-14 /pmc/articles/PMC4206947/ /pubmed/25338518 http://dx.doi.org/10.1038/srep06065 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Sancey, L.
Motto-Ros, V.
Busser, B.
Kotb, S.
Benoit, J. M.
Piednoir, A.
Lux, F.
Tillement, O.
Panczer, G.
Yu, J.
Laser spectrometry for multi-elemental imaging of biological tissues
title Laser spectrometry for multi-elemental imaging of biological tissues
title_full Laser spectrometry for multi-elemental imaging of biological tissues
title_fullStr Laser spectrometry for multi-elemental imaging of biological tissues
title_full_unstemmed Laser spectrometry for multi-elemental imaging of biological tissues
title_short Laser spectrometry for multi-elemental imaging of biological tissues
title_sort laser spectrometry for multi-elemental imaging of biological tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206947/
https://www.ncbi.nlm.nih.gov/pubmed/25338518
http://dx.doi.org/10.1038/srep06065
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