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Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS

The burden of neurodegenerative disorders in an aging population has become a challenge for the modern world. While the biomarkers available and the methods of diagnosis have improved to detect the onset of these diseases at early stages, the question of adapted and efficient therapies is still a ma...

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Autores principales: Viero, Cedric, Forostyak, Oksana, Sykova, Eva, Dayanithi, Govindan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207039/
https://www.ncbi.nlm.nih.gov/pubmed/25364743
http://dx.doi.org/10.3389/fcell.2014.00036
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author Viero, Cedric
Forostyak, Oksana
Sykova, Eva
Dayanithi, Govindan
author_facet Viero, Cedric
Forostyak, Oksana
Sykova, Eva
Dayanithi, Govindan
author_sort Viero, Cedric
collection PubMed
description The burden of neurodegenerative disorders in an aging population has become a challenge for the modern world. While the biomarkers available and the methods of diagnosis have improved to detect the onset of these diseases at early stages, the question of adapted and efficient therapies is still a major issue. The prospect of replacing the loss of functional neural cells remains an attractive but still audacious approach. A huge progress has been made in the generation of neurons derived from human stem cell lines and transplantation assays are tested in animals for a wide range of pathologies of the central nervous system. Here we take one step back and examine neuronal differentiation and the characterization of neural progenitors derived from human embryonic stem cells. We gather results from our previous studies and present a cell model that was successfully used in functional analyses and engraftment experiments. These neuronal precursors exhibit spontaneous and evoked activity, indicating that their electrophysiological and calcium handling properties are similar to those of matured neurons. Hence this summarized information will serve as a basis to design better stem cell-based therapies to improve neural regeneration.
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spelling pubmed-42070392014-10-31 Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS Viero, Cedric Forostyak, Oksana Sykova, Eva Dayanithi, Govindan Front Cell Dev Biol Cell and Developmental Biology The burden of neurodegenerative disorders in an aging population has become a challenge for the modern world. While the biomarkers available and the methods of diagnosis have improved to detect the onset of these diseases at early stages, the question of adapted and efficient therapies is still a major issue. The prospect of replacing the loss of functional neural cells remains an attractive but still audacious approach. A huge progress has been made in the generation of neurons derived from human stem cell lines and transplantation assays are tested in animals for a wide range of pathologies of the central nervous system. Here we take one step back and examine neuronal differentiation and the characterization of neural progenitors derived from human embryonic stem cells. We gather results from our previous studies and present a cell model that was successfully used in functional analyses and engraftment experiments. These neuronal precursors exhibit spontaneous and evoked activity, indicating that their electrophysiological and calcium handling properties are similar to those of matured neurons. Hence this summarized information will serve as a basis to design better stem cell-based therapies to improve neural regeneration. Frontiers Media S.A. 2014-08-13 /pmc/articles/PMC4207039/ /pubmed/25364743 http://dx.doi.org/10.3389/fcell.2014.00036 Text en Copyright © 2014 Viero, Forostyak, Sykova and Dayanithi. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Viero, Cedric
Forostyak, Oksana
Sykova, Eva
Dayanithi, Govindan
Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS
title Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS
title_full Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS
title_fullStr Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS
title_full_unstemmed Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS
title_short Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS
title_sort getting it right before transplantation: example of a stem cell model with regenerative potential for the cns
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207039/
https://www.ncbi.nlm.nih.gov/pubmed/25364743
http://dx.doi.org/10.3389/fcell.2014.00036
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