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Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS
The burden of neurodegenerative disorders in an aging population has become a challenge for the modern world. While the biomarkers available and the methods of diagnosis have improved to detect the onset of these diseases at early stages, the question of adapted and efficient therapies is still a ma...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207039/ https://www.ncbi.nlm.nih.gov/pubmed/25364743 http://dx.doi.org/10.3389/fcell.2014.00036 |
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author | Viero, Cedric Forostyak, Oksana Sykova, Eva Dayanithi, Govindan |
author_facet | Viero, Cedric Forostyak, Oksana Sykova, Eva Dayanithi, Govindan |
author_sort | Viero, Cedric |
collection | PubMed |
description | The burden of neurodegenerative disorders in an aging population has become a challenge for the modern world. While the biomarkers available and the methods of diagnosis have improved to detect the onset of these diseases at early stages, the question of adapted and efficient therapies is still a major issue. The prospect of replacing the loss of functional neural cells remains an attractive but still audacious approach. A huge progress has been made in the generation of neurons derived from human stem cell lines and transplantation assays are tested in animals for a wide range of pathologies of the central nervous system. Here we take one step back and examine neuronal differentiation and the characterization of neural progenitors derived from human embryonic stem cells. We gather results from our previous studies and present a cell model that was successfully used in functional analyses and engraftment experiments. These neuronal precursors exhibit spontaneous and evoked activity, indicating that their electrophysiological and calcium handling properties are similar to those of matured neurons. Hence this summarized information will serve as a basis to design better stem cell-based therapies to improve neural regeneration. |
format | Online Article Text |
id | pubmed-4207039 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-42070392014-10-31 Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS Viero, Cedric Forostyak, Oksana Sykova, Eva Dayanithi, Govindan Front Cell Dev Biol Cell and Developmental Biology The burden of neurodegenerative disorders in an aging population has become a challenge for the modern world. While the biomarkers available and the methods of diagnosis have improved to detect the onset of these diseases at early stages, the question of adapted and efficient therapies is still a major issue. The prospect of replacing the loss of functional neural cells remains an attractive but still audacious approach. A huge progress has been made in the generation of neurons derived from human stem cell lines and transplantation assays are tested in animals for a wide range of pathologies of the central nervous system. Here we take one step back and examine neuronal differentiation and the characterization of neural progenitors derived from human embryonic stem cells. We gather results from our previous studies and present a cell model that was successfully used in functional analyses and engraftment experiments. These neuronal precursors exhibit spontaneous and evoked activity, indicating that their electrophysiological and calcium handling properties are similar to those of matured neurons. Hence this summarized information will serve as a basis to design better stem cell-based therapies to improve neural regeneration. Frontiers Media S.A. 2014-08-13 /pmc/articles/PMC4207039/ /pubmed/25364743 http://dx.doi.org/10.3389/fcell.2014.00036 Text en Copyright © 2014 Viero, Forostyak, Sykova and Dayanithi. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Viero, Cedric Forostyak, Oksana Sykova, Eva Dayanithi, Govindan Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS |
title | Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS |
title_full | Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS |
title_fullStr | Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS |
title_full_unstemmed | Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS |
title_short | Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS |
title_sort | getting it right before transplantation: example of a stem cell model with regenerative potential for the cns |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207039/ https://www.ncbi.nlm.nih.gov/pubmed/25364743 http://dx.doi.org/10.3389/fcell.2014.00036 |
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