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Detection of oxygen addition peaks for terpendole E and related indole–diterpene alkaloids in a positive-mode ESI-MS
This report describes that a regular positive electrospray ionization mass spectrometry (MS) analysis of terpendoles often causes unexpected oxygen additions to form [M + H + O](+) and [M + H + 2O](+), which might be a troublesome in the characterization of new natural analogues. The intensities of...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207197/ https://www.ncbi.nlm.nih.gov/pubmed/24913406 http://dx.doi.org/10.1002/jms.3360 |
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author | Hongo, Yayoi Nakamura, Takemichi Takahashi, Shunya Motoyama, Takayuki Hayashi, Toshiaki Hirota, Hiroshi Osada, Hiroyuki Koshino, Hiroyuki |
author_facet | Hongo, Yayoi Nakamura, Takemichi Takahashi, Shunya Motoyama, Takayuki Hayashi, Toshiaki Hirota, Hiroshi Osada, Hiroyuki Koshino, Hiroyuki |
author_sort | Hongo, Yayoi |
collection | PubMed |
description | This report describes that a regular positive electrospray ionization mass spectrometry (MS) analysis of terpendoles often causes unexpected oxygen additions to form [M + H + O](+) and [M + H + 2O](+), which might be a troublesome in the characterization of new natural analogues. The intensities of [M + H + O](+) and [M + H + 2O](+) among terpendoles were unpredictable and fluctuated largely. Simple electrochemical oxidation in electrospray ionization was insufficient to explain the phenomenon. So we studied factors to form [M + H + O](+) and [M + H + 2O](+) using terpendole E and natural terpendoles together with some model indole alkaloids. Similar oxygen addition was observed for 1,2,3,4-tetrahydrocyclopent[b]indole, which is corresponding to the substructure of terpendole E. In tandem MS experiments, a major fragment ion at m/z 130 from protonated terpendole E was assigned to the substructure containing indole. When the [M + H + O](+) was selected as a precursor ion, the ion shifted to m/z 146. The same 16 Da shift of fragments was also observed for 1,2,3,4-tetrahydrocyclopent[b]indole, indicating that the oxygen addition of terpendole E took place at the indole portion. However, the oxygen addition was absent for some terpendoles, even whose structure resembles terpendole E. The breakdown curves characterized the tandem MS features of terpendoles. Preferential dissociation into m/z 130 suggested the protonation tendency at the indole site. Terpendoles that are preferentially protonated at indole tend to form oxygen addition peaks, suggesting that the protonation feature contributes to the oxygen additions in some degrees. © 2014 The Authors. Journal of Mass Spectrometry published by John Wiley & Sons, Ltd. |
format | Online Article Text |
id | pubmed-4207197 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-42071972014-11-13 Detection of oxygen addition peaks for terpendole E and related indole–diterpene alkaloids in a positive-mode ESI-MS Hongo, Yayoi Nakamura, Takemichi Takahashi, Shunya Motoyama, Takayuki Hayashi, Toshiaki Hirota, Hiroshi Osada, Hiroyuki Koshino, Hiroyuki J Mass Spectrom Jms Letters This report describes that a regular positive electrospray ionization mass spectrometry (MS) analysis of terpendoles often causes unexpected oxygen additions to form [M + H + O](+) and [M + H + 2O](+), which might be a troublesome in the characterization of new natural analogues. The intensities of [M + H + O](+) and [M + H + 2O](+) among terpendoles were unpredictable and fluctuated largely. Simple electrochemical oxidation in electrospray ionization was insufficient to explain the phenomenon. So we studied factors to form [M + H + O](+) and [M + H + 2O](+) using terpendole E and natural terpendoles together with some model indole alkaloids. Similar oxygen addition was observed for 1,2,3,4-tetrahydrocyclopent[b]indole, which is corresponding to the substructure of terpendole E. In tandem MS experiments, a major fragment ion at m/z 130 from protonated terpendole E was assigned to the substructure containing indole. When the [M + H + O](+) was selected as a precursor ion, the ion shifted to m/z 146. The same 16 Da shift of fragments was also observed for 1,2,3,4-tetrahydrocyclopent[b]indole, indicating that the oxygen addition of terpendole E took place at the indole portion. However, the oxygen addition was absent for some terpendoles, even whose structure resembles terpendole E. The breakdown curves characterized the tandem MS features of terpendoles. Preferential dissociation into m/z 130 suggested the protonation tendency at the indole site. Terpendoles that are preferentially protonated at indole tend to form oxygen addition peaks, suggesting that the protonation feature contributes to the oxygen additions in some degrees. © 2014 The Authors. Journal of Mass Spectrometry published by John Wiley & Sons, Ltd. Blackwell Publishing Ltd 2014-06 2014-05-21 /pmc/articles/PMC4207197/ /pubmed/24913406 http://dx.doi.org/10.1002/jms.3360 Text en © 2014 The Authors. Journal of Mass Spectrometry published by John Wiley & Sons, Ltd. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Jms Letters Hongo, Yayoi Nakamura, Takemichi Takahashi, Shunya Motoyama, Takayuki Hayashi, Toshiaki Hirota, Hiroshi Osada, Hiroyuki Koshino, Hiroyuki Detection of oxygen addition peaks for terpendole E and related indole–diterpene alkaloids in a positive-mode ESI-MS |
title | Detection of oxygen addition peaks for terpendole E and related indole–diterpene alkaloids in a positive-mode ESI-MS |
title_full | Detection of oxygen addition peaks for terpendole E and related indole–diterpene alkaloids in a positive-mode ESI-MS |
title_fullStr | Detection of oxygen addition peaks for terpendole E and related indole–diterpene alkaloids in a positive-mode ESI-MS |
title_full_unstemmed | Detection of oxygen addition peaks for terpendole E and related indole–diterpene alkaloids in a positive-mode ESI-MS |
title_short | Detection of oxygen addition peaks for terpendole E and related indole–diterpene alkaloids in a positive-mode ESI-MS |
title_sort | detection of oxygen addition peaks for terpendole e and related indole–diterpene alkaloids in a positive-mode esi-ms |
topic | Jms Letters |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207197/ https://www.ncbi.nlm.nih.gov/pubmed/24913406 http://dx.doi.org/10.1002/jms.3360 |
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