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Heterozygous SOD2 Deletion Impairs Glucose-Stimulated Insulin Secretion, but Not Insulin Action, in High-Fat–Fed Mice

Elevated reactive oxygen species (ROS) are linked to insulin resistance and islet dysfunction. Manganese superoxide dismutase (SOD2) is a primary defense against mitochondrial oxidative stress. To test the hypothesis that heterozygous SOD2 deletion impairs glucose-stimulated insulin secretion (GSIS)...

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Autores principales: Kang, Li, Dai, Chunhua, Lustig, Mary E., Bonner, Jeffrey S., Mayes, Wesley H., Mokshagundam, Shilpa, James, Freyja D., Thompson, Courtney S., Lin, Chien-Te, Perry, Christopher G.R., Anderson, Ethan J., Neufer, P. Darrell, Wasserman, David H., Powers, Alvin C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Diabetes Association 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207395/
https://www.ncbi.nlm.nih.gov/pubmed/24947366
http://dx.doi.org/10.2337/db13-1845
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author Kang, Li
Dai, Chunhua
Lustig, Mary E.
Bonner, Jeffrey S.
Mayes, Wesley H.
Mokshagundam, Shilpa
James, Freyja D.
Thompson, Courtney S.
Lin, Chien-Te
Perry, Christopher G.R.
Anderson, Ethan J.
Neufer, P. Darrell
Wasserman, David H.
Powers, Alvin C.
author_facet Kang, Li
Dai, Chunhua
Lustig, Mary E.
Bonner, Jeffrey S.
Mayes, Wesley H.
Mokshagundam, Shilpa
James, Freyja D.
Thompson, Courtney S.
Lin, Chien-Te
Perry, Christopher G.R.
Anderson, Ethan J.
Neufer, P. Darrell
Wasserman, David H.
Powers, Alvin C.
author_sort Kang, Li
collection PubMed
description Elevated reactive oxygen species (ROS) are linked to insulin resistance and islet dysfunction. Manganese superoxide dismutase (SOD2) is a primary defense against mitochondrial oxidative stress. To test the hypothesis that heterozygous SOD2 deletion impairs glucose-stimulated insulin secretion (GSIS) and insulin action, wild-type (sod2(+/+)) and heterozygous knockout mice (sod2(+/−)) were fed a chow or high-fat (HF) diet, which accelerates ROS production. Hyperglycemic (HG) and hyperinsulinemic-euglycemic (HI) clamps were performed to assess GSIS and insulin action in vivo. GSIS during HG clamps was equal in chow-fed sod2(+/−) and sod2(+/+) but was markedly decreased in HF-fed sod2(+/−). Remarkably, this impairment was not paralleled by reduced HG glucose infusion rate (GIR). Decreased GSIS in HF-fed sod2(+/−) was associated with increased ROS, such as superoxide ion. Surprisingly, insulin action determined by HI clamps did not differ between sod2(+/−) and sod2(+/+) of either diet. Since insulin action was unaffected, we hypothesized that the unchanged HG GIR in HF-fed sod2(+/−) was due to increased glucose effectiveness. Increased GLUT-1, hexokinase II, and phospho-AMPK protein in muscle of HF-fed sod2(+/−) support this hypothesis. We conclude that heterozygous SOD2 deletion in mice, a model that mimics SOD2 changes observed in diabetic humans, impairs GSIS in HF-fed mice without affecting insulin action.
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spelling pubmed-42073952015-11-01 Heterozygous SOD2 Deletion Impairs Glucose-Stimulated Insulin Secretion, but Not Insulin Action, in High-Fat–Fed Mice Kang, Li Dai, Chunhua Lustig, Mary E. Bonner, Jeffrey S. Mayes, Wesley H. Mokshagundam, Shilpa James, Freyja D. Thompson, Courtney S. Lin, Chien-Te Perry, Christopher G.R. Anderson, Ethan J. Neufer, P. Darrell Wasserman, David H. Powers, Alvin C. Diabetes Metabolism Elevated reactive oxygen species (ROS) are linked to insulin resistance and islet dysfunction. Manganese superoxide dismutase (SOD2) is a primary defense against mitochondrial oxidative stress. To test the hypothesis that heterozygous SOD2 deletion impairs glucose-stimulated insulin secretion (GSIS) and insulin action, wild-type (sod2(+/+)) and heterozygous knockout mice (sod2(+/−)) were fed a chow or high-fat (HF) diet, which accelerates ROS production. Hyperglycemic (HG) and hyperinsulinemic-euglycemic (HI) clamps were performed to assess GSIS and insulin action in vivo. GSIS during HG clamps was equal in chow-fed sod2(+/−) and sod2(+/+) but was markedly decreased in HF-fed sod2(+/−). Remarkably, this impairment was not paralleled by reduced HG glucose infusion rate (GIR). Decreased GSIS in HF-fed sod2(+/−) was associated with increased ROS, such as superoxide ion. Surprisingly, insulin action determined by HI clamps did not differ between sod2(+/−) and sod2(+/+) of either diet. Since insulin action was unaffected, we hypothesized that the unchanged HG GIR in HF-fed sod2(+/−) was due to increased glucose effectiveness. Increased GLUT-1, hexokinase II, and phospho-AMPK protein in muscle of HF-fed sod2(+/−) support this hypothesis. We conclude that heterozygous SOD2 deletion in mice, a model that mimics SOD2 changes observed in diabetic humans, impairs GSIS in HF-fed mice without affecting insulin action. American Diabetes Association 2014-11 2014-10-13 /pmc/articles/PMC4207395/ /pubmed/24947366 http://dx.doi.org/10.2337/db13-1845 Text en © 2014 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.
spellingShingle Metabolism
Kang, Li
Dai, Chunhua
Lustig, Mary E.
Bonner, Jeffrey S.
Mayes, Wesley H.
Mokshagundam, Shilpa
James, Freyja D.
Thompson, Courtney S.
Lin, Chien-Te
Perry, Christopher G.R.
Anderson, Ethan J.
Neufer, P. Darrell
Wasserman, David H.
Powers, Alvin C.
Heterozygous SOD2 Deletion Impairs Glucose-Stimulated Insulin Secretion, but Not Insulin Action, in High-Fat–Fed Mice
title Heterozygous SOD2 Deletion Impairs Glucose-Stimulated Insulin Secretion, but Not Insulin Action, in High-Fat–Fed Mice
title_full Heterozygous SOD2 Deletion Impairs Glucose-Stimulated Insulin Secretion, but Not Insulin Action, in High-Fat–Fed Mice
title_fullStr Heterozygous SOD2 Deletion Impairs Glucose-Stimulated Insulin Secretion, but Not Insulin Action, in High-Fat–Fed Mice
title_full_unstemmed Heterozygous SOD2 Deletion Impairs Glucose-Stimulated Insulin Secretion, but Not Insulin Action, in High-Fat–Fed Mice
title_short Heterozygous SOD2 Deletion Impairs Glucose-Stimulated Insulin Secretion, but Not Insulin Action, in High-Fat–Fed Mice
title_sort heterozygous sod2 deletion impairs glucose-stimulated insulin secretion, but not insulin action, in high-fat–fed mice
topic Metabolism
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207395/
https://www.ncbi.nlm.nih.gov/pubmed/24947366
http://dx.doi.org/10.2337/db13-1845
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