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The Photoprotective Role of Spermidine in Tomato Seedlings under Salinity-Alkalinity Stress

Polyamines are small, ubiquitous, nitrogenous compounds that scavenge reactive oxygen species and stabilize the structure and function of the photosynthetic apparatus in response to abiotic stresses. Molecular details underlying polyamine-mediated photoprotective mechanisms are not completely resolv...

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Autores principales: Hu, Lipan, Xiang, Lixia, Zhang, Li, Zhou, Xiaoting, Zou, Zhirong, Hu, Xiaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207769/
https://www.ncbi.nlm.nih.gov/pubmed/25340351
http://dx.doi.org/10.1371/journal.pone.0110855
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author Hu, Lipan
Xiang, Lixia
Zhang, Li
Zhou, Xiaoting
Zou, Zhirong
Hu, Xiaohui
author_facet Hu, Lipan
Xiang, Lixia
Zhang, Li
Zhou, Xiaoting
Zou, Zhirong
Hu, Xiaohui
author_sort Hu, Lipan
collection PubMed
description Polyamines are small, ubiquitous, nitrogenous compounds that scavenge reactive oxygen species and stabilize the structure and function of the photosynthetic apparatus in response to abiotic stresses. Molecular details underlying polyamine-mediated photoprotective mechanisms are not completely resolved. This study investigated the role of spermidine (Spd) in the structure and function of the photosynthetic apparatus. Tomato seedlings were subjected to salinity-alkalinity stress with and without foliar application of Spd, and photosynthetic and morphological parameters were analyzed. Leaf dry weight and net photosynthetic rate were reduced by salinity-alkalinity stress. Salinity-alkalinity stress reduced photochemical quenching parameters, including maximum photochemistry efficiency of photosystem II, quantum yield of linear electron flux, and coefficient of photochemical quenching (qP). Salinity-alkalinity stress elevated nonphotochemical quenching parameters, including the de-epoxidation state of the xanthophyll cycle and nonphotochemical quenching (NPQ). Microscopic analysis revealed that salinity-alkalinity stress disrupted the internal lamellar system of granal and stromal thylakoids. Exogenous Spd alleviated the stress-induced reduction of leaf dry weight, net photosynthetic rate, and qP parameters. The NPQ parameters increased by salinity-alkalinity stress were also alleviated by Spd. Seedlings treated with exogenous Spd had higher zeaxanthin (Z) contents than those without Spd under salinity-alkalinity stress. The chloroplast ultrastructure had a more ordered arrangement in seedlings treated with exogenous Spd than in those without Spd under salinity-alkalinity stress. These results indicate that exogenous Spd can alleviate the growth inhibition and thylakoid membrane photodamage caused by salinity-alkalinity stress. The Spd-induced accumulation of Z also may have an important role in stabilizing the photosynthetic apparatus.
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spelling pubmed-42077692014-10-27 The Photoprotective Role of Spermidine in Tomato Seedlings under Salinity-Alkalinity Stress Hu, Lipan Xiang, Lixia Zhang, Li Zhou, Xiaoting Zou, Zhirong Hu, Xiaohui PLoS One Research Article Polyamines are small, ubiquitous, nitrogenous compounds that scavenge reactive oxygen species and stabilize the structure and function of the photosynthetic apparatus in response to abiotic stresses. Molecular details underlying polyamine-mediated photoprotective mechanisms are not completely resolved. This study investigated the role of spermidine (Spd) in the structure and function of the photosynthetic apparatus. Tomato seedlings were subjected to salinity-alkalinity stress with and without foliar application of Spd, and photosynthetic and morphological parameters were analyzed. Leaf dry weight and net photosynthetic rate were reduced by salinity-alkalinity stress. Salinity-alkalinity stress reduced photochemical quenching parameters, including maximum photochemistry efficiency of photosystem II, quantum yield of linear electron flux, and coefficient of photochemical quenching (qP). Salinity-alkalinity stress elevated nonphotochemical quenching parameters, including the de-epoxidation state of the xanthophyll cycle and nonphotochemical quenching (NPQ). Microscopic analysis revealed that salinity-alkalinity stress disrupted the internal lamellar system of granal and stromal thylakoids. Exogenous Spd alleviated the stress-induced reduction of leaf dry weight, net photosynthetic rate, and qP parameters. The NPQ parameters increased by salinity-alkalinity stress were also alleviated by Spd. Seedlings treated with exogenous Spd had higher zeaxanthin (Z) contents than those without Spd under salinity-alkalinity stress. The chloroplast ultrastructure had a more ordered arrangement in seedlings treated with exogenous Spd than in those without Spd under salinity-alkalinity stress. These results indicate that exogenous Spd can alleviate the growth inhibition and thylakoid membrane photodamage caused by salinity-alkalinity stress. The Spd-induced accumulation of Z also may have an important role in stabilizing the photosynthetic apparatus. Public Library of Science 2014-10-23 /pmc/articles/PMC4207769/ /pubmed/25340351 http://dx.doi.org/10.1371/journal.pone.0110855 Text en © 2014 Hu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hu, Lipan
Xiang, Lixia
Zhang, Li
Zhou, Xiaoting
Zou, Zhirong
Hu, Xiaohui
The Photoprotective Role of Spermidine in Tomato Seedlings under Salinity-Alkalinity Stress
title The Photoprotective Role of Spermidine in Tomato Seedlings under Salinity-Alkalinity Stress
title_full The Photoprotective Role of Spermidine in Tomato Seedlings under Salinity-Alkalinity Stress
title_fullStr The Photoprotective Role of Spermidine in Tomato Seedlings under Salinity-Alkalinity Stress
title_full_unstemmed The Photoprotective Role of Spermidine in Tomato Seedlings under Salinity-Alkalinity Stress
title_short The Photoprotective Role of Spermidine in Tomato Seedlings under Salinity-Alkalinity Stress
title_sort photoprotective role of spermidine in tomato seedlings under salinity-alkalinity stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207769/
https://www.ncbi.nlm.nih.gov/pubmed/25340351
http://dx.doi.org/10.1371/journal.pone.0110855
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