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Elevated Peritoneal Expression and Estrogen Regulation of Nociceptive Ion Channels in Endometriosis
CONTEXT: Ovarian suppression is a common treatment for endometriosis-associated pelvic pain. Its exact mechanism of action is poorly understood, although it is assumed to reflect reduced production/action of estrogens. OBJECTIVE: The objective of the study was to measure the expression of mRNAs enco...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Endocrine Society
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207935/ https://www.ncbi.nlm.nih.gov/pubmed/25029427 http://dx.doi.org/10.1210/jc.2014-2282 |
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author | Greaves, Erin Grieve, Kelsey Horne, Andrew W. Saunders, Philippa T. K. |
author_facet | Greaves, Erin Grieve, Kelsey Horne, Andrew W. Saunders, Philippa T. K. |
author_sort | Greaves, Erin |
collection | PubMed |
description | CONTEXT: Ovarian suppression is a common treatment for endometriosis-associated pelvic pain. Its exact mechanism of action is poorly understood, although it is assumed to reflect reduced production/action of estrogens. OBJECTIVE: The objective of the study was to measure the expression of mRNAs encoded by nociceptive genes in the peritoneum of women with chronic pelvic pain (CPP) with or without endometriosis and to investigate whether estrogens alter nociceptive gene expression in human sensory neurons. DESIGN: The study was performed using human tissue analysis and cell culture. SETTING: The study was conducted at a university research institute. PATIENTS: Peritoneal biopsies were obtained from women with CPP and endometriosis (n = 12), CPP and no endometriosis (n = 10), and no pain or endometriosis (n = 5). Endometriosis lesions were obtained from women with endometriosis (n = 18). MAIN OUTCOME MEASURES: mRNAs encoding ion channels (P2RX3, SCN9A, SCN11A, TRPA1, TRPV1) and the neurotransmitter TAC1 were measured in human tissue samples and in human embryonic stem cell-derived sensory neurons treated with estrogens. RESULTS: TRPV1, TRPA1, and SCN11A mRNAs were significantly higher in the peritoneum from women with endometriosis (P < .001, P < .01). TRPV1, SCN9A, and TAC1 were elevated in endometriosis lesions (P < .05). P2RX3 mRNA was increased in the peritoneum of women with CPP, with and without endometriosis (P < .05). Incubation of sensory neurons with 17β-estradiol increased TRPV1 mRNA (P < .01). The estrogen receptor-β-selective agonist 2,3-bis(4-hydroxy-phenyl)-propionitrile increased concentrations of TRPV1, P2RX3, SCN9A, and TAC1 mRNAs. CONCLUSIONS: Estrogen-dependent expression of TRPV1 in sensory neurons may explain why ovarian suppression can reduce endometriosis-associated pain. Strategies directly targeting ion channels may offer an alternative option for the management of CPP. |
format | Online Article Text |
id | pubmed-4207935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Endocrine Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-42079352014-11-04 Elevated Peritoneal Expression and Estrogen Regulation of Nociceptive Ion Channels in Endometriosis Greaves, Erin Grieve, Kelsey Horne, Andrew W. Saunders, Philippa T. K. J Clin Endocrinol Metab JCEM Online: Brief Reports CONTEXT: Ovarian suppression is a common treatment for endometriosis-associated pelvic pain. Its exact mechanism of action is poorly understood, although it is assumed to reflect reduced production/action of estrogens. OBJECTIVE: The objective of the study was to measure the expression of mRNAs encoded by nociceptive genes in the peritoneum of women with chronic pelvic pain (CPP) with or without endometriosis and to investigate whether estrogens alter nociceptive gene expression in human sensory neurons. DESIGN: The study was performed using human tissue analysis and cell culture. SETTING: The study was conducted at a university research institute. PATIENTS: Peritoneal biopsies were obtained from women with CPP and endometriosis (n = 12), CPP and no endometriosis (n = 10), and no pain or endometriosis (n = 5). Endometriosis lesions were obtained from women with endometriosis (n = 18). MAIN OUTCOME MEASURES: mRNAs encoding ion channels (P2RX3, SCN9A, SCN11A, TRPA1, TRPV1) and the neurotransmitter TAC1 were measured in human tissue samples and in human embryonic stem cell-derived sensory neurons treated with estrogens. RESULTS: TRPV1, TRPA1, and SCN11A mRNAs were significantly higher in the peritoneum from women with endometriosis (P < .001, P < .01). TRPV1, SCN9A, and TAC1 were elevated in endometriosis lesions (P < .05). P2RX3 mRNA was increased in the peritoneum of women with CPP, with and without endometriosis (P < .05). Incubation of sensory neurons with 17β-estradiol increased TRPV1 mRNA (P < .01). The estrogen receptor-β-selective agonist 2,3-bis(4-hydroxy-phenyl)-propionitrile increased concentrations of TRPV1, P2RX3, SCN9A, and TAC1 mRNAs. CONCLUSIONS: Estrogen-dependent expression of TRPV1 in sensory neurons may explain why ovarian suppression can reduce endometriosis-associated pain. Strategies directly targeting ion channels may offer an alternative option for the management of CPP. Endocrine Society 2014-09 2014-07-16 /pmc/articles/PMC4207935/ /pubmed/25029427 http://dx.doi.org/10.1210/jc.2014-2282 Text en Copyright © 2014 by the Endocrine Society This article has been published under the terms of the Creative Commons Attribution License (CC-BY (http://creativecommons.org/licenses/by/3.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright for this article is retained by the author(s). Author(s) grant(s) the Endocrine Society the exclusive right to publish the article and identify itself as the original publisher. |
spellingShingle | JCEM Online: Brief Reports Greaves, Erin Grieve, Kelsey Horne, Andrew W. Saunders, Philippa T. K. Elevated Peritoneal Expression and Estrogen Regulation of Nociceptive Ion Channels in Endometriosis |
title | Elevated Peritoneal Expression and Estrogen Regulation of Nociceptive Ion Channels in Endometriosis |
title_full | Elevated Peritoneal Expression and Estrogen Regulation of Nociceptive Ion Channels in Endometriosis |
title_fullStr | Elevated Peritoneal Expression and Estrogen Regulation of Nociceptive Ion Channels in Endometriosis |
title_full_unstemmed | Elevated Peritoneal Expression and Estrogen Regulation of Nociceptive Ion Channels in Endometriosis |
title_short | Elevated Peritoneal Expression and Estrogen Regulation of Nociceptive Ion Channels in Endometriosis |
title_sort | elevated peritoneal expression and estrogen regulation of nociceptive ion channels in endometriosis |
topic | JCEM Online: Brief Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207935/ https://www.ncbi.nlm.nih.gov/pubmed/25029427 http://dx.doi.org/10.1210/jc.2014-2282 |
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