Biologically Active Constituents from Salix viminalis Bio-Oil and Their Protective Activity Against Hydrogen Peroxide-Induced Oxidative Stress in Chinese Hamster Ovary Cells

The protective antioxidative effect of the phenolic extract (PE) isolated from Salix viminalis pyrolysis derived bio-oil was shown in vitro on the Chinese hamster ovary (CHO) cells exposed to hydrogen peroxide (H(2)O(2)). Cells pretreated with 0.05 μg/ml PE after exposure to different concentrations...

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Detalles Bibliográficos
Autores principales: Ilnicka, Anna, Roszek, Katarzyna, Olejniczak, Andrzej, Komoszynski, Michal, Lukaszewicz, Jerzy P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207959/
https://www.ncbi.nlm.nih.gov/pubmed/25172057
http://dx.doi.org/10.1007/s12010-014-1171-0
Descripción
Sumario:The protective antioxidative effect of the phenolic extract (PE) isolated from Salix viminalis pyrolysis derived bio-oil was shown in vitro on the Chinese hamster ovary (CHO) cells exposed to hydrogen peroxide (H(2)O(2)). Cells pretreated with 0.05 μg/ml PE after exposure to different concentrations of H(2)O(2) (300–900 μM) showed up to 25 % higher viability than the unpretreated ones. The antioxidative effect of PE was also observed in a time-dependent manner. The results were confirmed by visual examination of the specimens using microscopy. Finally, superoxide dismutase (SOD) activity modulation was shown by SOD assay, designed to determine the activity of enzymes removing free radicals.

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