Biologically Active Constituents from Salix viminalis Bio-Oil and Their Protective Activity Against Hydrogen Peroxide-Induced Oxidative Stress in Chinese Hamster Ovary Cells

The protective antioxidative effect of the phenolic extract (PE) isolated from Salix viminalis pyrolysis derived bio-oil was shown in vitro on the Chinese hamster ovary (CHO) cells exposed to hydrogen peroxide (H(2)O(2)). Cells pretreated with 0.05 μg/ml PE after exposure to different concentrations...

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Autores principales: Ilnicka, Anna, Roszek, Katarzyna, Olejniczak, Andrzej, Komoszynski, Michal, Lukaszewicz, Jerzy P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207959/
https://www.ncbi.nlm.nih.gov/pubmed/25172057
http://dx.doi.org/10.1007/s12010-014-1171-0
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author Ilnicka, Anna
Roszek, Katarzyna
Olejniczak, Andrzej
Komoszynski, Michal
Lukaszewicz, Jerzy P.
author_facet Ilnicka, Anna
Roszek, Katarzyna
Olejniczak, Andrzej
Komoszynski, Michal
Lukaszewicz, Jerzy P.
author_sort Ilnicka, Anna
collection PubMed
description The protective antioxidative effect of the phenolic extract (PE) isolated from Salix viminalis pyrolysis derived bio-oil was shown in vitro on the Chinese hamster ovary (CHO) cells exposed to hydrogen peroxide (H(2)O(2)). Cells pretreated with 0.05 μg/ml PE after exposure to different concentrations of H(2)O(2) (300–900 μM) showed up to 25 % higher viability than the unpretreated ones. The antioxidative effect of PE was also observed in a time-dependent manner. The results were confirmed by visual examination of the specimens using microscopy. Finally, superoxide dismutase (SOD) activity modulation was shown by SOD assay, designed to determine the activity of enzymes removing free radicals.
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spelling pubmed-42079592014-10-28 Biologically Active Constituents from Salix viminalis Bio-Oil and Their Protective Activity Against Hydrogen Peroxide-Induced Oxidative Stress in Chinese Hamster Ovary Cells Ilnicka, Anna Roszek, Katarzyna Olejniczak, Andrzej Komoszynski, Michal Lukaszewicz, Jerzy P. Appl Biochem Biotechnol Article The protective antioxidative effect of the phenolic extract (PE) isolated from Salix viminalis pyrolysis derived bio-oil was shown in vitro on the Chinese hamster ovary (CHO) cells exposed to hydrogen peroxide (H(2)O(2)). Cells pretreated with 0.05 μg/ml PE after exposure to different concentrations of H(2)O(2) (300–900 μM) showed up to 25 % higher viability than the unpretreated ones. The antioxidative effect of PE was also observed in a time-dependent manner. The results were confirmed by visual examination of the specimens using microscopy. Finally, superoxide dismutase (SOD) activity modulation was shown by SOD assay, designed to determine the activity of enzymes removing free radicals. Springer US 2014-08-30 2014 /pmc/articles/PMC4207959/ /pubmed/25172057 http://dx.doi.org/10.1007/s12010-014-1171-0 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Ilnicka, Anna
Roszek, Katarzyna
Olejniczak, Andrzej
Komoszynski, Michal
Lukaszewicz, Jerzy P.
Biologically Active Constituents from Salix viminalis Bio-Oil and Their Protective Activity Against Hydrogen Peroxide-Induced Oxidative Stress in Chinese Hamster Ovary Cells
title Biologically Active Constituents from Salix viminalis Bio-Oil and Their Protective Activity Against Hydrogen Peroxide-Induced Oxidative Stress in Chinese Hamster Ovary Cells
title_full Biologically Active Constituents from Salix viminalis Bio-Oil and Their Protective Activity Against Hydrogen Peroxide-Induced Oxidative Stress in Chinese Hamster Ovary Cells
title_fullStr Biologically Active Constituents from Salix viminalis Bio-Oil and Their Protective Activity Against Hydrogen Peroxide-Induced Oxidative Stress in Chinese Hamster Ovary Cells
title_full_unstemmed Biologically Active Constituents from Salix viminalis Bio-Oil and Their Protective Activity Against Hydrogen Peroxide-Induced Oxidative Stress in Chinese Hamster Ovary Cells
title_short Biologically Active Constituents from Salix viminalis Bio-Oil and Their Protective Activity Against Hydrogen Peroxide-Induced Oxidative Stress in Chinese Hamster Ovary Cells
title_sort biologically active constituents from salix viminalis bio-oil and their protective activity against hydrogen peroxide-induced oxidative stress in chinese hamster ovary cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207959/
https://www.ncbi.nlm.nih.gov/pubmed/25172057
http://dx.doi.org/10.1007/s12010-014-1171-0
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