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Decreased Frequencies of Circulating CD4(+) T Follicular Helper Cells Associated with Diminished Plasma IL-21 in Active Pulmonary Tuberculosis
BACKGROUND: Circulating T follicular helper (Tfh) cells represent a distinct subset of CD4(+) T cells and are important in immunity to infections. Although they have been shown to play a role in experimental models of tuberculosis infection, their role in human tuberculosis remains unexplored. AIMS/...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4208798/ https://www.ncbi.nlm.nih.gov/pubmed/25343703 http://dx.doi.org/10.1371/journal.pone.0111098 |
Sumario: | BACKGROUND: Circulating T follicular helper (Tfh) cells represent a distinct subset of CD4(+) T cells and are important in immunity to infections. Although they have been shown to play a role in experimental models of tuberculosis infection, their role in human tuberculosis remains unexplored. AIMS/METHODOLOGY: To determine the distribution of circulating Tfh cells in human TB, we measured the frequencies of Tfh cells ex vivo and following TB - antigen or polyclonal stimulation in pulmonary TB (PTB; n = 30) and latent TB (LTB; n = 20) individuals, using the markers CXCR5, PD-1 and ICOS. RESULTS: We found that both ex vivo and TB - antigen induced frequencies of Tfh cell subsets was significantly lower in PTB compared to LTB individuals. Similarly, antigen induced frequencies of Tfh cells expressing IL-21 was also significantly lower in PTB individuals and this was reflected in diminished circulating levels of IL-21 and IFNγ. This was not accompanied by diminished frequencies of activated or memory B cell subsets. Finally, the diminution in frequency of Tfh cells in PTB individuals was dependent on IL-10, CTLA-4 and PD-L1 in vitro. CONCLUSIONS: Thus, PTB is characterized by adiminution in the frequency of Tfh cell subsets. |
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