Harmonisation of short-term in vitro culture for the expansion of antigen-specific CD8(+) T cells with detection by ELISPOT and HLA-multimer staining

Ex vivo ELISPOT and multimer staining are well-established tests for the assessment of antigen-specific T cells. Many laboratories are now using a period of in vitro stimulation (IVS) to enhance detection. Here, we report the findings of a multi-centre panel organised by the Association for Cancer I...

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Detalles Bibliográficos
Autores principales: Chudley, Lindsey, McCann, Katy J., Coleman, Adam, Cazaly, Angelica M., Bidmon, Nicole, Britten, Cedrik M., van der Burg, Sjoerd H., Gouttefangeas, Cecile, Jandus, Camilla, Laske, Karoline, Maurer, Dominik, Romero, Pedro, Schröder, Helene, Stynenbosch, Linda F. M., Walter, Steffen, Welters, Marij J. P., Ottensmeier, Christian H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4209099/
https://www.ncbi.nlm.nih.gov/pubmed/25134947
http://dx.doi.org/10.1007/s00262-014-1593-0

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