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miR-155 induced transcriptome changes in the MCF-7 breast cancer cell line leads to enhanced mitogen activated protein kinase signaling

A single microRNA (miRNA) has the potential to regulate thousands of genes and thus govern multiple signaling pathways at once. miR-155 is an oncogenic miRNA which regulates many cellular pathways, designating it as a multifaceted regulator of proliferation, chemo-resistance, and apoptosis. While ma...

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Autores principales: Martin, Elizabeth C., Krebs, Adrienne E., Burks, Hope E., Elliott, Steven, Baddoo, Melody, Collins-Burow, Bridgette M., Flemington, Erik K., Burow, Matthew E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4209600/
https://www.ncbi.nlm.nih.gov/pubmed/25352952
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author Martin, Elizabeth C.
Krebs, Adrienne E.
Burks, Hope E.
Elliott, Steven
Baddoo, Melody
Collins-Burow, Bridgette M.
Flemington, Erik K.
Burow, Matthew E.
author_facet Martin, Elizabeth C.
Krebs, Adrienne E.
Burks, Hope E.
Elliott, Steven
Baddoo, Melody
Collins-Burow, Bridgette M.
Flemington, Erik K.
Burow, Matthew E.
author_sort Martin, Elizabeth C.
collection PubMed
description A single microRNA (miRNA) has the potential to regulate thousands of genes and thus govern multiple signaling pathways at once. miR-155 is an oncogenic miRNA which regulates many cellular pathways, designating it as a multifaceted regulator of proliferation, chemo-resistance, and apoptosis. While many singular targeted effects of miR-155 have been defined and an oncogenic role has been attributed to miR-155 expression, the global effect of miR-155 on the cellular transcriptomes of an ER(+) breast cancer cell line has yet to be determined. Here we demonstrate that miR-155 expression increases tumorigenesis in vivo and we determine miR-155 mediated transcriptome changes through next generation sequencing analysis. miR-155 expression alters many signaling pathways, with the chief altered pathway being the MAPK signaling cascade and miR-155 induces shortening of target mRNA 3′UTRs and alternative isoform expression of MAPK related genes. In addition there is an observed increase in protein phosphorylation of components of MAPK signaling including ERK1/2 and AP-1 complex members (Fra-1 and c-Fos) as well as elevated gene expression of MAPK regulated genes Zeb1, Snail, Plaur, and SerpinE1.
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spelling pubmed-42096002014-10-28 miR-155 induced transcriptome changes in the MCF-7 breast cancer cell line leads to enhanced mitogen activated protein kinase signaling Martin, Elizabeth C. Krebs, Adrienne E. Burks, Hope E. Elliott, Steven Baddoo, Melody Collins-Burow, Bridgette M. Flemington, Erik K. Burow, Matthew E. Genes Cancer Research Paper A single microRNA (miRNA) has the potential to regulate thousands of genes and thus govern multiple signaling pathways at once. miR-155 is an oncogenic miRNA which regulates many cellular pathways, designating it as a multifaceted regulator of proliferation, chemo-resistance, and apoptosis. While many singular targeted effects of miR-155 have been defined and an oncogenic role has been attributed to miR-155 expression, the global effect of miR-155 on the cellular transcriptomes of an ER(+) breast cancer cell line has yet to be determined. Here we demonstrate that miR-155 expression increases tumorigenesis in vivo and we determine miR-155 mediated transcriptome changes through next generation sequencing analysis. miR-155 expression alters many signaling pathways, with the chief altered pathway being the MAPK signaling cascade and miR-155 induces shortening of target mRNA 3′UTRs and alternative isoform expression of MAPK related genes. In addition there is an observed increase in protein phosphorylation of components of MAPK signaling including ERK1/2 and AP-1 complex members (Fra-1 and c-Fos) as well as elevated gene expression of MAPK regulated genes Zeb1, Snail, Plaur, and SerpinE1. Impact Journals LLC 2014-09 /pmc/articles/PMC4209600/ /pubmed/25352952 Text en Copyright: © 2014 Martin et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Martin, Elizabeth C.
Krebs, Adrienne E.
Burks, Hope E.
Elliott, Steven
Baddoo, Melody
Collins-Burow, Bridgette M.
Flemington, Erik K.
Burow, Matthew E.
miR-155 induced transcriptome changes in the MCF-7 breast cancer cell line leads to enhanced mitogen activated protein kinase signaling
title miR-155 induced transcriptome changes in the MCF-7 breast cancer cell line leads to enhanced mitogen activated protein kinase signaling
title_full miR-155 induced transcriptome changes in the MCF-7 breast cancer cell line leads to enhanced mitogen activated protein kinase signaling
title_fullStr miR-155 induced transcriptome changes in the MCF-7 breast cancer cell line leads to enhanced mitogen activated protein kinase signaling
title_full_unstemmed miR-155 induced transcriptome changes in the MCF-7 breast cancer cell line leads to enhanced mitogen activated protein kinase signaling
title_short miR-155 induced transcriptome changes in the MCF-7 breast cancer cell line leads to enhanced mitogen activated protein kinase signaling
title_sort mir-155 induced transcriptome changes in the mcf-7 breast cancer cell line leads to enhanced mitogen activated protein kinase signaling
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4209600/
https://www.ncbi.nlm.nih.gov/pubmed/25352952
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