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Staphylococcus aureus ST398 gene expression profiling during ex vivo colonization of porcine nasal epithelium

BACKGROUND: Staphylococcus aureus is a common human and animal opportunistic pathogen. In humans nasal carriage of S. aureus is a risk factor for various infections. Methicillin-resistant S. aureus ST398 is highly prevalent in pigs in Europe and North America. The mechanism of successful pig coloniz...

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Autores principales: Tulinski, Pawel, Duim, Birgitta, Wittink, Floyd R, Jonker, Martijs J, Breit, Timo M, van Putten, Jos P, Wagenaar, Jaap A, Fluit, Ad C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210494/
https://www.ncbi.nlm.nih.gov/pubmed/25331735
http://dx.doi.org/10.1186/1471-2164-15-915
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author Tulinski, Pawel
Duim, Birgitta
Wittink, Floyd R
Jonker, Martijs J
Breit, Timo M
van Putten, Jos P
Wagenaar, Jaap A
Fluit, Ad C
author_facet Tulinski, Pawel
Duim, Birgitta
Wittink, Floyd R
Jonker, Martijs J
Breit, Timo M
van Putten, Jos P
Wagenaar, Jaap A
Fluit, Ad C
author_sort Tulinski, Pawel
collection PubMed
description BACKGROUND: Staphylococcus aureus is a common human and animal opportunistic pathogen. In humans nasal carriage of S. aureus is a risk factor for various infections. Methicillin-resistant S. aureus ST398 is highly prevalent in pigs in Europe and North America. The mechanism of successful pig colonization by MRSA ST398 is poorly understood. Previously, we developed a nasal colonization model of porcine nasal mucosa explants to identify molecular traits involved in nasal MRSA colonization of pigs. RESULTS: We report the analysis of changes in the transcription of MRSA ST398 strain S0462 during colonization on the explant epithelium. Major regulated genes were encoding metabolic processes and regulation of these genes may represent metabolic adaptation to nasal mucosa explants. Colonization was not accompanied by significant changes in transcripts of the main virulence associated genes or known human colonization factors. Here, we documented regulation of two genes which have potential influence on S. aureus colonization; cysteine extracellular proteinase (scpA) and von Willebrand factor-binding protein (vWbp, encoded on SaPIbov5). Colonization with isogenic-deletion strains (Δvwbp and ΔscpA) did not alter the ex vivo nasal S. aureus colonization compared to wild type. CONCLUSIONS: Our results suggest that nasal colonization with MRSA ST398 is a complex event that is accompanied with changes in bacterial gene expression regulation and metabolic adaptation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-915) contains supplementary material, which is available to authorized users.
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spelling pubmed-42104942014-10-29 Staphylococcus aureus ST398 gene expression profiling during ex vivo colonization of porcine nasal epithelium Tulinski, Pawel Duim, Birgitta Wittink, Floyd R Jonker, Martijs J Breit, Timo M van Putten, Jos P Wagenaar, Jaap A Fluit, Ad C BMC Genomics Research Article BACKGROUND: Staphylococcus aureus is a common human and animal opportunistic pathogen. In humans nasal carriage of S. aureus is a risk factor for various infections. Methicillin-resistant S. aureus ST398 is highly prevalent in pigs in Europe and North America. The mechanism of successful pig colonization by MRSA ST398 is poorly understood. Previously, we developed a nasal colonization model of porcine nasal mucosa explants to identify molecular traits involved in nasal MRSA colonization of pigs. RESULTS: We report the analysis of changes in the transcription of MRSA ST398 strain S0462 during colonization on the explant epithelium. Major regulated genes were encoding metabolic processes and regulation of these genes may represent metabolic adaptation to nasal mucosa explants. Colonization was not accompanied by significant changes in transcripts of the main virulence associated genes or known human colonization factors. Here, we documented regulation of two genes which have potential influence on S. aureus colonization; cysteine extracellular proteinase (scpA) and von Willebrand factor-binding protein (vWbp, encoded on SaPIbov5). Colonization with isogenic-deletion strains (Δvwbp and ΔscpA) did not alter the ex vivo nasal S. aureus colonization compared to wild type. CONCLUSIONS: Our results suggest that nasal colonization with MRSA ST398 is a complex event that is accompanied with changes in bacterial gene expression regulation and metabolic adaptation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-915) contains supplementary material, which is available to authorized users. BioMed Central 2014-10-20 /pmc/articles/PMC4210494/ /pubmed/25331735 http://dx.doi.org/10.1186/1471-2164-15-915 Text en © Tulinski et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Tulinski, Pawel
Duim, Birgitta
Wittink, Floyd R
Jonker, Martijs J
Breit, Timo M
van Putten, Jos P
Wagenaar, Jaap A
Fluit, Ad C
Staphylococcus aureus ST398 gene expression profiling during ex vivo colonization of porcine nasal epithelium
title Staphylococcus aureus ST398 gene expression profiling during ex vivo colonization of porcine nasal epithelium
title_full Staphylococcus aureus ST398 gene expression profiling during ex vivo colonization of porcine nasal epithelium
title_fullStr Staphylococcus aureus ST398 gene expression profiling during ex vivo colonization of porcine nasal epithelium
title_full_unstemmed Staphylococcus aureus ST398 gene expression profiling during ex vivo colonization of porcine nasal epithelium
title_short Staphylococcus aureus ST398 gene expression profiling during ex vivo colonization of porcine nasal epithelium
title_sort staphylococcus aureus st398 gene expression profiling during ex vivo colonization of porcine nasal epithelium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210494/
https://www.ncbi.nlm.nih.gov/pubmed/25331735
http://dx.doi.org/10.1186/1471-2164-15-915
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