Polygenic in vivo validation of cancer mutations using transposons

The in vivo validation of cancer mutations and genes identified in cancer genomics is resource-intensive because of the low throughput of animal experiments. We describe a mouse model that allows multiple cancer mutations to be validated in each animal line. Animal lines are generated with multiple...

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Autores principales: Chew, Su Kit, Lu, Dong, Campos, Lia S, Scott, Kenneth L, Saci, Abdel, Wang, Juexuan, Collinson, Adam, Raine, Keiran, Hinton, Jonathan, Teague, Jon W, Jones, David, Menzies, Andrew, Butler, Adam P, Gamble, John, O’Meara, Sarah, McLaren, Stuart, Chin, Lynda, Liu, Pentao, Futreal, P Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210617/
https://www.ncbi.nlm.nih.gov/pubmed/25260652
http://dx.doi.org/10.1186/s13059-014-0455-6
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author Chew, Su Kit
Lu, Dong
Campos, Lia S
Scott, Kenneth L
Saci, Abdel
Wang, Juexuan
Collinson, Adam
Raine, Keiran
Hinton, Jonathan
Teague, Jon W
Jones, David
Menzies, Andrew
Butler, Adam P
Gamble, John
O’Meara, Sarah
McLaren, Stuart
Chin, Lynda
Liu, Pentao
Futreal, P Andrew
author_facet Chew, Su Kit
Lu, Dong
Campos, Lia S
Scott, Kenneth L
Saci, Abdel
Wang, Juexuan
Collinson, Adam
Raine, Keiran
Hinton, Jonathan
Teague, Jon W
Jones, David
Menzies, Andrew
Butler, Adam P
Gamble, John
O’Meara, Sarah
McLaren, Stuart
Chin, Lynda
Liu, Pentao
Futreal, P Andrew
author_sort Chew, Su Kit
collection PubMed
description The in vivo validation of cancer mutations and genes identified in cancer genomics is resource-intensive because of the low throughput of animal experiments. We describe a mouse model that allows multiple cancer mutations to be validated in each animal line. Animal lines are generated with multiple candidate cancer mutations using transposons. The candidate cancer genes are tagged and randomly expressed in somatic cells, allowing easy identification of the cancer genes involved in the generated tumours. This system presents a useful, generalised and efficient means for animal validation of cancer genes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13059-014-0455-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-42106172014-10-29 Polygenic in vivo validation of cancer mutations using transposons Chew, Su Kit Lu, Dong Campos, Lia S Scott, Kenneth L Saci, Abdel Wang, Juexuan Collinson, Adam Raine, Keiran Hinton, Jonathan Teague, Jon W Jones, David Menzies, Andrew Butler, Adam P Gamble, John O’Meara, Sarah McLaren, Stuart Chin, Lynda Liu, Pentao Futreal, P Andrew Genome Biol Method The in vivo validation of cancer mutations and genes identified in cancer genomics is resource-intensive because of the low throughput of animal experiments. We describe a mouse model that allows multiple cancer mutations to be validated in each animal line. Animal lines are generated with multiple candidate cancer mutations using transposons. The candidate cancer genes are tagged and randomly expressed in somatic cells, allowing easy identification of the cancer genes involved in the generated tumours. This system presents a useful, generalised and efficient means for animal validation of cancer genes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13059-014-0455-6) contains supplementary material, which is available to authorized users. BioMed Central 2014-09-27 2014 /pmc/articles/PMC4210617/ /pubmed/25260652 http://dx.doi.org/10.1186/s13059-014-0455-6 Text en © Chew et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Method
Chew, Su Kit
Lu, Dong
Campos, Lia S
Scott, Kenneth L
Saci, Abdel
Wang, Juexuan
Collinson, Adam
Raine, Keiran
Hinton, Jonathan
Teague, Jon W
Jones, David
Menzies, Andrew
Butler, Adam P
Gamble, John
O’Meara, Sarah
McLaren, Stuart
Chin, Lynda
Liu, Pentao
Futreal, P Andrew
Polygenic in vivo validation of cancer mutations using transposons
title Polygenic in vivo validation of cancer mutations using transposons
title_full Polygenic in vivo validation of cancer mutations using transposons
title_fullStr Polygenic in vivo validation of cancer mutations using transposons
title_full_unstemmed Polygenic in vivo validation of cancer mutations using transposons
title_short Polygenic in vivo validation of cancer mutations using transposons
title_sort polygenic in vivo validation of cancer mutations using transposons
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210617/
https://www.ncbi.nlm.nih.gov/pubmed/25260652
http://dx.doi.org/10.1186/s13059-014-0455-6
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