Cargando…

Anti-Inflammation Activities of Mycosporine-Like Amino Acids (MAAs) in Response to UV Radiation Suggest Potential Anti-Skin Aging Activity

Certain photosynthetic marine organisms have evolved mechanisms to counteract UV-radiation by synthesizing UV-absorbing compounds, such as mycosporine-like amino acids (MAAs). In this study, MAAs were separated from the extracts of marine green alga Chlamydomonas hedleyi using HPLC and were identifi...

Descripción completa

Detalles Bibliográficos
Autores principales: Suh, Sung-Suk, Hwang, Jinik, Park, Mirye, Seo, Hyo Hyun, Kim, Hyoung-Shik, Lee, Jeong Hun, Moh, Sang Hyun, Lee, Taek-Kyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210892/
https://www.ncbi.nlm.nih.gov/pubmed/25317535
http://dx.doi.org/10.3390/md12105174
_version_ 1782341466760151040
author Suh, Sung-Suk
Hwang, Jinik
Park, Mirye
Seo, Hyo Hyun
Kim, Hyoung-Shik
Lee, Jeong Hun
Moh, Sang Hyun
Lee, Taek-Kyun
author_facet Suh, Sung-Suk
Hwang, Jinik
Park, Mirye
Seo, Hyo Hyun
Kim, Hyoung-Shik
Lee, Jeong Hun
Moh, Sang Hyun
Lee, Taek-Kyun
author_sort Suh, Sung-Suk
collection PubMed
description Certain photosynthetic marine organisms have evolved mechanisms to counteract UV-radiation by synthesizing UV-absorbing compounds, such as mycosporine-like amino acids (MAAs). In this study, MAAs were separated from the extracts of marine green alga Chlamydomonas hedleyi using HPLC and were identified as porphyra-334, shinorine, and mycosporine-glycine (mycosporine-Gly), based on their retention times and maximum absorption wavelengths. Furthermore, their structures were confirmed by triple quadrupole MS/MS. Their roles as UV-absorbing compounds were investigated in the human fibroblast cell line HaCaT by analyzing the expression levels of genes associated with antioxidant activity, inflammation, and skin aging in response to UV irradiation. The mycosporine-Gly extract, but not the other MAAs, had strong antioxidant activity in the 2,2-diphenyl-1-picryhydrazyl (DPPH) assay. Furthermore, treatment with mycosporine-Gly resulted in a significant decrease in COX-2 mRNA levels, which are typically increased in response to inflammation in the skin, in a concentration-dependent manner. Additionally, in the presence of MAAs, the UV-suppressed genes, procollagen C proteinase enhancer (PCOLCE) and elastin, which are related to skin aging, had increased expression levels equal to those in UV-mock treated cells. Interestingly, the increased expression of involucrin after UV exposure was suppressed by treatment with the MAAs mycosporine-Gly and shinorine, but not porphyra-334. This is the first report investigating the biological activities of microalgae-derived MAAs in human cells.
format Online
Article
Text
id pubmed-4210892
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-42108922014-10-28 Anti-Inflammation Activities of Mycosporine-Like Amino Acids (MAAs) in Response to UV Radiation Suggest Potential Anti-Skin Aging Activity Suh, Sung-Suk Hwang, Jinik Park, Mirye Seo, Hyo Hyun Kim, Hyoung-Shik Lee, Jeong Hun Moh, Sang Hyun Lee, Taek-Kyun Mar Drugs Article Certain photosynthetic marine organisms have evolved mechanisms to counteract UV-radiation by synthesizing UV-absorbing compounds, such as mycosporine-like amino acids (MAAs). In this study, MAAs were separated from the extracts of marine green alga Chlamydomonas hedleyi using HPLC and were identified as porphyra-334, shinorine, and mycosporine-glycine (mycosporine-Gly), based on their retention times and maximum absorption wavelengths. Furthermore, their structures were confirmed by triple quadrupole MS/MS. Their roles as UV-absorbing compounds were investigated in the human fibroblast cell line HaCaT by analyzing the expression levels of genes associated with antioxidant activity, inflammation, and skin aging in response to UV irradiation. The mycosporine-Gly extract, but not the other MAAs, had strong antioxidant activity in the 2,2-diphenyl-1-picryhydrazyl (DPPH) assay. Furthermore, treatment with mycosporine-Gly resulted in a significant decrease in COX-2 mRNA levels, which are typically increased in response to inflammation in the skin, in a concentration-dependent manner. Additionally, in the presence of MAAs, the UV-suppressed genes, procollagen C proteinase enhancer (PCOLCE) and elastin, which are related to skin aging, had increased expression levels equal to those in UV-mock treated cells. Interestingly, the increased expression of involucrin after UV exposure was suppressed by treatment with the MAAs mycosporine-Gly and shinorine, but not porphyra-334. This is the first report investigating the biological activities of microalgae-derived MAAs in human cells. MDPI 2014-10-14 /pmc/articles/PMC4210892/ /pubmed/25317535 http://dx.doi.org/10.3390/md12105174 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Suh, Sung-Suk
Hwang, Jinik
Park, Mirye
Seo, Hyo Hyun
Kim, Hyoung-Shik
Lee, Jeong Hun
Moh, Sang Hyun
Lee, Taek-Kyun
Anti-Inflammation Activities of Mycosporine-Like Amino Acids (MAAs) in Response to UV Radiation Suggest Potential Anti-Skin Aging Activity
title Anti-Inflammation Activities of Mycosporine-Like Amino Acids (MAAs) in Response to UV Radiation Suggest Potential Anti-Skin Aging Activity
title_full Anti-Inflammation Activities of Mycosporine-Like Amino Acids (MAAs) in Response to UV Radiation Suggest Potential Anti-Skin Aging Activity
title_fullStr Anti-Inflammation Activities of Mycosporine-Like Amino Acids (MAAs) in Response to UV Radiation Suggest Potential Anti-Skin Aging Activity
title_full_unstemmed Anti-Inflammation Activities of Mycosporine-Like Amino Acids (MAAs) in Response to UV Radiation Suggest Potential Anti-Skin Aging Activity
title_short Anti-Inflammation Activities of Mycosporine-Like Amino Acids (MAAs) in Response to UV Radiation Suggest Potential Anti-Skin Aging Activity
title_sort anti-inflammation activities of mycosporine-like amino acids (maas) in response to uv radiation suggest potential anti-skin aging activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210892/
https://www.ncbi.nlm.nih.gov/pubmed/25317535
http://dx.doi.org/10.3390/md12105174
work_keys_str_mv AT suhsungsuk antiinflammationactivitiesofmycosporinelikeaminoacidsmaasinresponsetouvradiationsuggestpotentialantiskinagingactivity
AT hwangjinik antiinflammationactivitiesofmycosporinelikeaminoacidsmaasinresponsetouvradiationsuggestpotentialantiskinagingactivity
AT parkmirye antiinflammationactivitiesofmycosporinelikeaminoacidsmaasinresponsetouvradiationsuggestpotentialantiskinagingactivity
AT seohyohyun antiinflammationactivitiesofmycosporinelikeaminoacidsmaasinresponsetouvradiationsuggestpotentialantiskinagingactivity
AT kimhyoungshik antiinflammationactivitiesofmycosporinelikeaminoacidsmaasinresponsetouvradiationsuggestpotentialantiskinagingactivity
AT leejeonghun antiinflammationactivitiesofmycosporinelikeaminoacidsmaasinresponsetouvradiationsuggestpotentialantiskinagingactivity
AT mohsanghyun antiinflammationactivitiesofmycosporinelikeaminoacidsmaasinresponsetouvradiationsuggestpotentialantiskinagingactivity
AT leetaekkyun antiinflammationactivitiesofmycosporinelikeaminoacidsmaasinresponsetouvradiationsuggestpotentialantiskinagingactivity