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Nutrients Intake Is Associated with DNA Methylation of Candidate Inflammatory Genes in a Population of Obese Subjects

The aim of the present study was to evaluate the potential association between dietary nutrients and alterations in DNA methylation in a set of five candidate genes, including CD14, Et-1, iNOS, HERV-w and TNFα, in a population of overweight/obese subjects. We evaluated possible associations between...

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Autores principales: Bollati, Valentina, Favero, Chiara, Albetti, Benedetta, Tarantini, Letizia, Moroni, Alice, Byun, Hyang-Min, Motta, Valeria, Conti, Diana Misaela, Tirelli, Amedea Silvia, Vigna, Luisella, Bertazzi, Pier Alberto, Pesatori, Angela Cecilia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210937/
https://www.ncbi.nlm.nih.gov/pubmed/25340371
http://dx.doi.org/10.3390/nu6104625
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author Bollati, Valentina
Favero, Chiara
Albetti, Benedetta
Tarantini, Letizia
Moroni, Alice
Byun, Hyang-Min
Motta, Valeria
Conti, Diana Misaela
Tirelli, Amedea Silvia
Vigna, Luisella
Bertazzi, Pier Alberto
Pesatori, Angela Cecilia
author_facet Bollati, Valentina
Favero, Chiara
Albetti, Benedetta
Tarantini, Letizia
Moroni, Alice
Byun, Hyang-Min
Motta, Valeria
Conti, Diana Misaela
Tirelli, Amedea Silvia
Vigna, Luisella
Bertazzi, Pier Alberto
Pesatori, Angela Cecilia
author_sort Bollati, Valentina
collection PubMed
description The aim of the present study was to evaluate the potential association between dietary nutrients and alterations in DNA methylation in a set of five candidate genes, including CD14, Et-1, iNOS, HERV-w and TNFα, in a population of overweight/obese subjects. We evaluated possible associations between gene methylation and clinical blood parameters, including total cholesterol (TC), low- and high-density lipoprotein cholesterol (LDL-C and HDL-C), triglyceride and homocysteine levels. We employed validated methods to assess anthropometric, clinical and dietary data, as well as pyrosequencing to evaluate DNA methylation of the five candidate genes in 165 overweight/obese subjects. There was no association between body mass index and DNA methylation of the five candidate genes in this group of subjects. Positive associations were observed between TNFα methylation and blood levels of LDL-C (β = 0.447, p = 0.002), TC/HDL-C (β = 0.467, p = 0.001) and LDL-C/HDL-C (β = 0.445, p = 0.002), as well as between HERV-w methylation and dietary intakes of β-carotene (β = 0.088, p = 0.051) and carotenoids (β = 0.083, p = 0.029). TNFα methylation showed negative associations with dietary intakes of cholesterol (β = −0.278, p = 0.048), folic acid (β = −0.339, p = 0.012), β-carotene (β = −0.332, p = 0.045), carotenoids (β = −0.331, p = 0.015) and retinol (β = −0.360, p = 0.008). These results suggest a complex relationship among nutrient intake, oxidative stress and DNA methylation.
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spelling pubmed-42109372014-10-28 Nutrients Intake Is Associated with DNA Methylation of Candidate Inflammatory Genes in a Population of Obese Subjects Bollati, Valentina Favero, Chiara Albetti, Benedetta Tarantini, Letizia Moroni, Alice Byun, Hyang-Min Motta, Valeria Conti, Diana Misaela Tirelli, Amedea Silvia Vigna, Luisella Bertazzi, Pier Alberto Pesatori, Angela Cecilia Nutrients Article The aim of the present study was to evaluate the potential association between dietary nutrients and alterations in DNA methylation in a set of five candidate genes, including CD14, Et-1, iNOS, HERV-w and TNFα, in a population of overweight/obese subjects. We evaluated possible associations between gene methylation and clinical blood parameters, including total cholesterol (TC), low- and high-density lipoprotein cholesterol (LDL-C and HDL-C), triglyceride and homocysteine levels. We employed validated methods to assess anthropometric, clinical and dietary data, as well as pyrosequencing to evaluate DNA methylation of the five candidate genes in 165 overweight/obese subjects. There was no association between body mass index and DNA methylation of the five candidate genes in this group of subjects. Positive associations were observed between TNFα methylation and blood levels of LDL-C (β = 0.447, p = 0.002), TC/HDL-C (β = 0.467, p = 0.001) and LDL-C/HDL-C (β = 0.445, p = 0.002), as well as between HERV-w methylation and dietary intakes of β-carotene (β = 0.088, p = 0.051) and carotenoids (β = 0.083, p = 0.029). TNFα methylation showed negative associations with dietary intakes of cholesterol (β = −0.278, p = 0.048), folic acid (β = −0.339, p = 0.012), β-carotene (β = −0.332, p = 0.045), carotenoids (β = −0.331, p = 0.015) and retinol (β = −0.360, p = 0.008). These results suggest a complex relationship among nutrient intake, oxidative stress and DNA methylation. MDPI 2014-10-22 /pmc/articles/PMC4210937/ /pubmed/25340371 http://dx.doi.org/10.3390/nu6104625 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bollati, Valentina
Favero, Chiara
Albetti, Benedetta
Tarantini, Letizia
Moroni, Alice
Byun, Hyang-Min
Motta, Valeria
Conti, Diana Misaela
Tirelli, Amedea Silvia
Vigna, Luisella
Bertazzi, Pier Alberto
Pesatori, Angela Cecilia
Nutrients Intake Is Associated with DNA Methylation of Candidate Inflammatory Genes in a Population of Obese Subjects
title Nutrients Intake Is Associated with DNA Methylation of Candidate Inflammatory Genes in a Population of Obese Subjects
title_full Nutrients Intake Is Associated with DNA Methylation of Candidate Inflammatory Genes in a Population of Obese Subjects
title_fullStr Nutrients Intake Is Associated with DNA Methylation of Candidate Inflammatory Genes in a Population of Obese Subjects
title_full_unstemmed Nutrients Intake Is Associated with DNA Methylation of Candidate Inflammatory Genes in a Population of Obese Subjects
title_short Nutrients Intake Is Associated with DNA Methylation of Candidate Inflammatory Genes in a Population of Obese Subjects
title_sort nutrients intake is associated with dna methylation of candidate inflammatory genes in a population of obese subjects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210937/
https://www.ncbi.nlm.nih.gov/pubmed/25340371
http://dx.doi.org/10.3390/nu6104625
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