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Comparing the efficacy of coconut oil and xylene as a clearing agent in the histopathology laboratory

BACKGROUND: The commonly used clearing agent, xylene is supposed to be highly toxic and carcinogenic. As previous research studies have shown the effectiveness of different vegetable oils as clearants, this study was designed to evaluate the efficacy of coconut oil. MATERIALS AND METHODS: Two equal...

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Autores principales: Sermadi, Wajid, Prabhu, Sudeendra, Acharya, Swetha, Javali, SB
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211238/
https://www.ncbi.nlm.nih.gov/pubmed/25364179
http://dx.doi.org/10.4103/0973-029X.141348
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author Sermadi, Wajid
Prabhu, Sudeendra
Acharya, Swetha
Javali, SB
author_facet Sermadi, Wajid
Prabhu, Sudeendra
Acharya, Swetha
Javali, SB
author_sort Sermadi, Wajid
collection PubMed
description BACKGROUND: The commonly used clearing agent, xylene is supposed to be highly toxic and carcinogenic. As previous research studies have shown the effectiveness of different vegetable oils as clearants, this study was designed to evaluate the efficacy of coconut oil. MATERIALS AND METHODS: Two equal halves of 60 soft tissue specimens were processed simultaneously in xylene and coconut oil as clearing agents. The Xylene-treated specimens (XY-S) and Coconut oil–treated specimens (CO-S) were checked for gross and histological features and comparison was done between the two groups. RESULTS: Significant shrinkage was noted in XY-S compared to that in CO-S. No difference was found in either of the sections when checked for cellular details and staining quality. Morphometrically, there was significant reduction in the mean cell area in XY-S compared to that in CO-S. CONCLUSION: Coconut oil may be substituted for the highly hazardous xylene as a clearing agent without compromising the quality of histological details.
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spelling pubmed-42112382014-10-31 Comparing the efficacy of coconut oil and xylene as a clearing agent in the histopathology laboratory Sermadi, Wajid Prabhu, Sudeendra Acharya, Swetha Javali, SB J Oral Maxillofac Pathol Original Article BACKGROUND: The commonly used clearing agent, xylene is supposed to be highly toxic and carcinogenic. As previous research studies have shown the effectiveness of different vegetable oils as clearants, this study was designed to evaluate the efficacy of coconut oil. MATERIALS AND METHODS: Two equal halves of 60 soft tissue specimens were processed simultaneously in xylene and coconut oil as clearing agents. The Xylene-treated specimens (XY-S) and Coconut oil–treated specimens (CO-S) were checked for gross and histological features and comparison was done between the two groups. RESULTS: Significant shrinkage was noted in XY-S compared to that in CO-S. No difference was found in either of the sections when checked for cellular details and staining quality. Morphometrically, there was significant reduction in the mean cell area in XY-S compared to that in CO-S. CONCLUSION: Coconut oil may be substituted for the highly hazardous xylene as a clearing agent without compromising the quality of histological details. Medknow Publications & Media Pvt Ltd 2014-09 /pmc/articles/PMC4211238/ /pubmed/25364179 http://dx.doi.org/10.4103/0973-029X.141348 Text en Copyright: © Journal of Oral and Maxillofacial Pathology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Sermadi, Wajid
Prabhu, Sudeendra
Acharya, Swetha
Javali, SB
Comparing the efficacy of coconut oil and xylene as a clearing agent in the histopathology laboratory
title Comparing the efficacy of coconut oil and xylene as a clearing agent in the histopathology laboratory
title_full Comparing the efficacy of coconut oil and xylene as a clearing agent in the histopathology laboratory
title_fullStr Comparing the efficacy of coconut oil and xylene as a clearing agent in the histopathology laboratory
title_full_unstemmed Comparing the efficacy of coconut oil and xylene as a clearing agent in the histopathology laboratory
title_short Comparing the efficacy of coconut oil and xylene as a clearing agent in the histopathology laboratory
title_sort comparing the efficacy of coconut oil and xylene as a clearing agent in the histopathology laboratory
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211238/
https://www.ncbi.nlm.nih.gov/pubmed/25364179
http://dx.doi.org/10.4103/0973-029X.141348
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