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Systematic Analysis of Time-Series Gene Expression Data on Tumor Cell-Selective Apoptotic Responses to HDAC Inhibitors

SAHA (suberoylanilide hydroxamic acid or vorinostat) is the first nonselective histone deacetylase (HDAC) inhibitor approved by the US Food and Drug Administration (FDA). SAHA affects histone acetylation in chromatin and a variety of nonhistone substrates, thus influencing many cellular processes. I...

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Autores principales: Qi, Yun-feng, Huang, Yan-xin, Dong, Yan, Zheng, Li-hua, Bao, Yong-li, Sun, Lu-guo, Wu, Yin, Yu, Chun-lei, Jiang, Hong-yu, Li, Yu-xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211306/
https://www.ncbi.nlm.nih.gov/pubmed/25371703
http://dx.doi.org/10.1155/2014/867289
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author Qi, Yun-feng
Huang, Yan-xin
Dong, Yan
Zheng, Li-hua
Bao, Yong-li
Sun, Lu-guo
Wu, Yin
Yu, Chun-lei
Jiang, Hong-yu
Li, Yu-xin
author_facet Qi, Yun-feng
Huang, Yan-xin
Dong, Yan
Zheng, Li-hua
Bao, Yong-li
Sun, Lu-guo
Wu, Yin
Yu, Chun-lei
Jiang, Hong-yu
Li, Yu-xin
author_sort Qi, Yun-feng
collection PubMed
description SAHA (suberoylanilide hydroxamic acid or vorinostat) is the first nonselective histone deacetylase (HDAC) inhibitor approved by the US Food and Drug Administration (FDA). SAHA affects histone acetylation in chromatin and a variety of nonhistone substrates, thus influencing many cellular processes. In particularly, SAHA induces selective apoptosis of tumor cells, although the mechanism is not well understood. A series of microarray experiments was recently conducted to investigate tumor cell-selective proapoptotic transcriptional responses induced by SAHA. Based on that gene expression time series, we propose a novel framework for detailed analysis of the mechanism of tumor cell apoptosis selectively induced by SAHA. Our analyses indicated that SAHA selectively disrupted the DNA damage response, cell cycle, p53 expression, and mitochondrial integrity of tumor samples to induce selective tumor cell apoptosis. Our results suggest a possible regulation network. Our research extends the existing research.
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spelling pubmed-42113062014-11-04 Systematic Analysis of Time-Series Gene Expression Data on Tumor Cell-Selective Apoptotic Responses to HDAC Inhibitors Qi, Yun-feng Huang, Yan-xin Dong, Yan Zheng, Li-hua Bao, Yong-li Sun, Lu-guo Wu, Yin Yu, Chun-lei Jiang, Hong-yu Li, Yu-xin Comput Math Methods Med Research Article SAHA (suberoylanilide hydroxamic acid or vorinostat) is the first nonselective histone deacetylase (HDAC) inhibitor approved by the US Food and Drug Administration (FDA). SAHA affects histone acetylation in chromatin and a variety of nonhistone substrates, thus influencing many cellular processes. In particularly, SAHA induces selective apoptosis of tumor cells, although the mechanism is not well understood. A series of microarray experiments was recently conducted to investigate tumor cell-selective proapoptotic transcriptional responses induced by SAHA. Based on that gene expression time series, we propose a novel framework for detailed analysis of the mechanism of tumor cell apoptosis selectively induced by SAHA. Our analyses indicated that SAHA selectively disrupted the DNA damage response, cell cycle, p53 expression, and mitochondrial integrity of tumor samples to induce selective tumor cell apoptosis. Our results suggest a possible regulation network. Our research extends the existing research. Hindawi Publishing Corporation 2014 2014-10-13 /pmc/articles/PMC4211306/ /pubmed/25371703 http://dx.doi.org/10.1155/2014/867289 Text en Copyright © 2014 Yun-feng Qi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Qi, Yun-feng
Huang, Yan-xin
Dong, Yan
Zheng, Li-hua
Bao, Yong-li
Sun, Lu-guo
Wu, Yin
Yu, Chun-lei
Jiang, Hong-yu
Li, Yu-xin
Systematic Analysis of Time-Series Gene Expression Data on Tumor Cell-Selective Apoptotic Responses to HDAC Inhibitors
title Systematic Analysis of Time-Series Gene Expression Data on Tumor Cell-Selective Apoptotic Responses to HDAC Inhibitors
title_full Systematic Analysis of Time-Series Gene Expression Data on Tumor Cell-Selective Apoptotic Responses to HDAC Inhibitors
title_fullStr Systematic Analysis of Time-Series Gene Expression Data on Tumor Cell-Selective Apoptotic Responses to HDAC Inhibitors
title_full_unstemmed Systematic Analysis of Time-Series Gene Expression Data on Tumor Cell-Selective Apoptotic Responses to HDAC Inhibitors
title_short Systematic Analysis of Time-Series Gene Expression Data on Tumor Cell-Selective Apoptotic Responses to HDAC Inhibitors
title_sort systematic analysis of time-series gene expression data on tumor cell-selective apoptotic responses to hdac inhibitors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211306/
https://www.ncbi.nlm.nih.gov/pubmed/25371703
http://dx.doi.org/10.1155/2014/867289
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