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Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota
This study evaluated the breath CH(4) excretion and concentration of M. smithii in intestinal microbiota of schoolchildren from 2 slums. One hundred and eleven children from a slum near a sanitary landfill, 35 children of a slum located away from the sanitary landfill, and 32 children from a high so...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211324/ https://www.ncbi.nlm.nih.gov/pubmed/25374477 http://dx.doi.org/10.1155/2014/576249 |
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author | Bezerra de Araujo Filho, Humberto Silva Carmo-Rodrigues, Mirian Santos Mello, Carolina Cristina Fonseca Lahoz Melli, Lígia Tahan, Soraia Carlos Campos Pignatari, Antonio Batista de Morais, Mauro |
author_facet | Bezerra de Araujo Filho, Humberto Silva Carmo-Rodrigues, Mirian Santos Mello, Carolina Cristina Fonseca Lahoz Melli, Lígia Tahan, Soraia Carlos Campos Pignatari, Antonio Batista de Morais, Mauro |
author_sort | Bezerra de Araujo Filho, Humberto |
collection | PubMed |
description | This study evaluated the breath CH(4) excretion and concentration of M. smithii in intestinal microbiota of schoolchildren from 2 slums. One hundred and eleven children from a slum near a sanitary landfill, 35 children of a slum located away from the sanitary landfill, and 32 children from a high socioeconomic level school were included in the study. Real-time PCR was performed to quantify the M. smithii nifH gene and it was present in the microbiota of all the participating children, with higher (P < 0.05) concentrations in those who lived in the slum near the landfill (3.16 × 10(7) CFU/g of feces), comparing with the children from the slum away from the landfill (2.05 × 10(6) CFU/g of feces) and those from the high socioeconomic level group (3.93 × 10(5) CFU/g of feces). The prevalence of children who present breath methane was 53% in the slum near the landfill, 31% in the slum further away from the landfill and, 22% in the high socioeconomic level group. To live near a landfill is associated with higher concentrations of M. smithii in intestinal microbiota, comparing with those who live away from the landfill, regardless of their socioeconomics conditions. |
format | Online Article Text |
id | pubmed-4211324 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-42113242014-11-05 Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota Bezerra de Araujo Filho, Humberto Silva Carmo-Rodrigues, Mirian Santos Mello, Carolina Cristina Fonseca Lahoz Melli, Lígia Tahan, Soraia Carlos Campos Pignatari, Antonio Batista de Morais, Mauro Archaea Research Article This study evaluated the breath CH(4) excretion and concentration of M. smithii in intestinal microbiota of schoolchildren from 2 slums. One hundred and eleven children from a slum near a sanitary landfill, 35 children of a slum located away from the sanitary landfill, and 32 children from a high socioeconomic level school were included in the study. Real-time PCR was performed to quantify the M. smithii nifH gene and it was present in the microbiota of all the participating children, with higher (P < 0.05) concentrations in those who lived in the slum near the landfill (3.16 × 10(7) CFU/g of feces), comparing with the children from the slum away from the landfill (2.05 × 10(6) CFU/g of feces) and those from the high socioeconomic level group (3.93 × 10(5) CFU/g of feces). The prevalence of children who present breath methane was 53% in the slum near the landfill, 31% in the slum further away from the landfill and, 22% in the high socioeconomic level group. To live near a landfill is associated with higher concentrations of M. smithii in intestinal microbiota, comparing with those who live away from the landfill, regardless of their socioeconomics conditions. Hindawi Publishing Corporation 2014-10-13 /pmc/articles/PMC4211324/ /pubmed/25374477 http://dx.doi.org/10.1155/2014/576249 Text en Copyright © 2014 Humberto Bezerra de Araujo Filho et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Bezerra de Araujo Filho, Humberto Silva Carmo-Rodrigues, Mirian Santos Mello, Carolina Cristina Fonseca Lahoz Melli, Lígia Tahan, Soraia Carlos Campos Pignatari, Antonio Batista de Morais, Mauro Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota |
title | Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota |
title_full | Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota |
title_fullStr | Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota |
title_full_unstemmed | Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota |
title_short | Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota |
title_sort | children living near a sanitary landfill have increased breath methane and methanobrevibacter smithii in their intestinal microbiota |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211324/ https://www.ncbi.nlm.nih.gov/pubmed/25374477 http://dx.doi.org/10.1155/2014/576249 |
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