Cargando…

Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota

This study evaluated the breath CH(4) excretion and concentration of M. smithii in intestinal microbiota of schoolchildren from 2 slums. One hundred and eleven children from a slum near a sanitary landfill, 35 children of a slum located away from the sanitary landfill, and 32 children from a high so...

Descripción completa

Detalles Bibliográficos
Autores principales: Bezerra de Araujo Filho, Humberto, Silva Carmo-Rodrigues, Mirian, Santos Mello, Carolina, Cristina Fonseca Lahoz Melli, Lígia, Tahan, Soraia, Carlos Campos Pignatari, Antonio, Batista de Morais, Mauro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211324/
https://www.ncbi.nlm.nih.gov/pubmed/25374477
http://dx.doi.org/10.1155/2014/576249
_version_ 1782341553347362816
author Bezerra de Araujo Filho, Humberto
Silva Carmo-Rodrigues, Mirian
Santos Mello, Carolina
Cristina Fonseca Lahoz Melli, Lígia
Tahan, Soraia
Carlos Campos Pignatari, Antonio
Batista de Morais, Mauro
author_facet Bezerra de Araujo Filho, Humberto
Silva Carmo-Rodrigues, Mirian
Santos Mello, Carolina
Cristina Fonseca Lahoz Melli, Lígia
Tahan, Soraia
Carlos Campos Pignatari, Antonio
Batista de Morais, Mauro
author_sort Bezerra de Araujo Filho, Humberto
collection PubMed
description This study evaluated the breath CH(4) excretion and concentration of M. smithii in intestinal microbiota of schoolchildren from 2 slums. One hundred and eleven children from a slum near a sanitary landfill, 35 children of a slum located away from the sanitary landfill, and 32 children from a high socioeconomic level school were included in the study. Real-time PCR was performed to quantify the M. smithii nifH gene and it was present in the microbiota of all the participating children, with higher (P < 0.05) concentrations in those who lived in the slum near the landfill (3.16 × 10(7) CFU/g of feces), comparing with the children from the slum away from the landfill (2.05 × 10(6) CFU/g of feces) and those from the high socioeconomic level group (3.93 × 10(5) CFU/g of feces). The prevalence of children who present breath methane was 53% in the slum near the landfill, 31% in the slum further away from the landfill and, 22% in the high socioeconomic level group. To live near a landfill is associated with higher concentrations of M. smithii in intestinal microbiota, comparing with those who live away from the landfill, regardless of their socioeconomics conditions.
format Online
Article
Text
id pubmed-4211324
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-42113242014-11-05 Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota Bezerra de Araujo Filho, Humberto Silva Carmo-Rodrigues, Mirian Santos Mello, Carolina Cristina Fonseca Lahoz Melli, Lígia Tahan, Soraia Carlos Campos Pignatari, Antonio Batista de Morais, Mauro Archaea Research Article This study evaluated the breath CH(4) excretion and concentration of M. smithii in intestinal microbiota of schoolchildren from 2 slums. One hundred and eleven children from a slum near a sanitary landfill, 35 children of a slum located away from the sanitary landfill, and 32 children from a high socioeconomic level school were included in the study. Real-time PCR was performed to quantify the M. smithii nifH gene and it was present in the microbiota of all the participating children, with higher (P < 0.05) concentrations in those who lived in the slum near the landfill (3.16 × 10(7) CFU/g of feces), comparing with the children from the slum away from the landfill (2.05 × 10(6) CFU/g of feces) and those from the high socioeconomic level group (3.93 × 10(5) CFU/g of feces). The prevalence of children who present breath methane was 53% in the slum near the landfill, 31% in the slum further away from the landfill and, 22% in the high socioeconomic level group. To live near a landfill is associated with higher concentrations of M. smithii in intestinal microbiota, comparing with those who live away from the landfill, regardless of their socioeconomics conditions. Hindawi Publishing Corporation 2014-10-13 /pmc/articles/PMC4211324/ /pubmed/25374477 http://dx.doi.org/10.1155/2014/576249 Text en Copyright © 2014 Humberto Bezerra de Araujo Filho et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bezerra de Araujo Filho, Humberto
Silva Carmo-Rodrigues, Mirian
Santos Mello, Carolina
Cristina Fonseca Lahoz Melli, Lígia
Tahan, Soraia
Carlos Campos Pignatari, Antonio
Batista de Morais, Mauro
Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota
title Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota
title_full Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota
title_fullStr Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota
title_full_unstemmed Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota
title_short Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota
title_sort children living near a sanitary landfill have increased breath methane and methanobrevibacter smithii in their intestinal microbiota
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211324/
https://www.ncbi.nlm.nih.gov/pubmed/25374477
http://dx.doi.org/10.1155/2014/576249
work_keys_str_mv AT bezerradearaujofilhohumberto childrenlivingnearasanitarylandfillhaveincreasedbreathmethaneandmethanobrevibactersmithiiintheirintestinalmicrobiota
AT silvacarmorodriguesmirian childrenlivingnearasanitarylandfillhaveincreasedbreathmethaneandmethanobrevibactersmithiiintheirintestinalmicrobiota
AT santosmellocarolina childrenlivingnearasanitarylandfillhaveincreasedbreathmethaneandmethanobrevibactersmithiiintheirintestinalmicrobiota
AT cristinafonsecalahozmelliligia childrenlivingnearasanitarylandfillhaveincreasedbreathmethaneandmethanobrevibactersmithiiintheirintestinalmicrobiota
AT tahansoraia childrenlivingnearasanitarylandfillhaveincreasedbreathmethaneandmethanobrevibactersmithiiintheirintestinalmicrobiota
AT carloscampospignatariantonio childrenlivingnearasanitarylandfillhaveincreasedbreathmethaneandmethanobrevibactersmithiiintheirintestinalmicrobiota
AT batistademoraismauro childrenlivingnearasanitarylandfillhaveincreasedbreathmethaneandmethanobrevibactersmithiiintheirintestinalmicrobiota