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Impact of interspecific interactions on antimicrobial activity among soil bacteria
Certain bacterial species produce antimicrobial compounds only in the presence of a competing species. However, little is known on the frequency of interaction-mediated induction of antibiotic compound production in natural communities of soil bacteria. Here we developed a high-throughput method to...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211544/ https://www.ncbi.nlm.nih.gov/pubmed/25389421 http://dx.doi.org/10.3389/fmicb.2014.00567 |
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author | Tyc, Olaf van den Berg, Marlies Gerards, Saskia van Veen, Johannes A. Raaijmakers, Jos M. de Boer, Wietse Garbeva, Paolina |
author_facet | Tyc, Olaf van den Berg, Marlies Gerards, Saskia van Veen, Johannes A. Raaijmakers, Jos M. de Boer, Wietse Garbeva, Paolina |
author_sort | Tyc, Olaf |
collection | PubMed |
description | Certain bacterial species produce antimicrobial compounds only in the presence of a competing species. However, little is known on the frequency of interaction-mediated induction of antibiotic compound production in natural communities of soil bacteria. Here we developed a high-throughput method to screen for the production of antimicrobial activity by monocultures and pair-wise combinations of 146 phylogenetically different bacteria isolated from similar soil habitats. Growth responses of two human pathogenic model organisms, Escherichia coli WA321 and Staphylococcus aureus 533R4, were used to monitor antimicrobial activity. From all isolates, 33% showed antimicrobial activity only in monoculture and 42% showed activity only when tested in interactions. More bacterial isolates were active against S. aureus than against E. coli. The frequency of interaction-mediated induction of antimicrobial activity was 6% (154 interactions out of 2798) indicating that only a limited set of species combinations showed such activity. The screening revealed also interaction-mediated suppression of antimicrobial activity for 22% of all combinations tested. Whereas all patterns of antimicrobial activity (non-induced production, induced production and suppression) were seen for various bacterial classes, interaction-mediated induction of antimicrobial activity was more frequent for combinations of Flavobacteria and alpha- Proteobacteria. The results of our study give a first indication on the frequency of interference competitive interactions in natural soil bacterial communities which may forms a basis for selection of bacterial groups that are promising for the discovery of novel, cryptic antibiotics. |
format | Online Article Text |
id | pubmed-4211544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-42115442014-11-11 Impact of interspecific interactions on antimicrobial activity among soil bacteria Tyc, Olaf van den Berg, Marlies Gerards, Saskia van Veen, Johannes A. Raaijmakers, Jos M. de Boer, Wietse Garbeva, Paolina Front Microbiol Microbiology Certain bacterial species produce antimicrobial compounds only in the presence of a competing species. However, little is known on the frequency of interaction-mediated induction of antibiotic compound production in natural communities of soil bacteria. Here we developed a high-throughput method to screen for the production of antimicrobial activity by monocultures and pair-wise combinations of 146 phylogenetically different bacteria isolated from similar soil habitats. Growth responses of two human pathogenic model organisms, Escherichia coli WA321 and Staphylococcus aureus 533R4, were used to monitor antimicrobial activity. From all isolates, 33% showed antimicrobial activity only in monoculture and 42% showed activity only when tested in interactions. More bacterial isolates were active against S. aureus than against E. coli. The frequency of interaction-mediated induction of antimicrobial activity was 6% (154 interactions out of 2798) indicating that only a limited set of species combinations showed such activity. The screening revealed also interaction-mediated suppression of antimicrobial activity for 22% of all combinations tested. Whereas all patterns of antimicrobial activity (non-induced production, induced production and suppression) were seen for various bacterial classes, interaction-mediated induction of antimicrobial activity was more frequent for combinations of Flavobacteria and alpha- Proteobacteria. The results of our study give a first indication on the frequency of interference competitive interactions in natural soil bacterial communities which may forms a basis for selection of bacterial groups that are promising for the discovery of novel, cryptic antibiotics. Frontiers Media S.A. 2014-10-28 /pmc/articles/PMC4211544/ /pubmed/25389421 http://dx.doi.org/10.3389/fmicb.2014.00567 Text en Copyright © 2014 Tyc, van den Berg, Gerards, van Veen, Raaijmakers, de Boer and Garbeva. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Tyc, Olaf van den Berg, Marlies Gerards, Saskia van Veen, Johannes A. Raaijmakers, Jos M. de Boer, Wietse Garbeva, Paolina Impact of interspecific interactions on antimicrobial activity among soil bacteria |
title | Impact of interspecific interactions on antimicrobial activity among soil bacteria |
title_full | Impact of interspecific interactions on antimicrobial activity among soil bacteria |
title_fullStr | Impact of interspecific interactions on antimicrobial activity among soil bacteria |
title_full_unstemmed | Impact of interspecific interactions on antimicrobial activity among soil bacteria |
title_short | Impact of interspecific interactions on antimicrobial activity among soil bacteria |
title_sort | impact of interspecific interactions on antimicrobial activity among soil bacteria |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211544/ https://www.ncbi.nlm.nih.gov/pubmed/25389421 http://dx.doi.org/10.3389/fmicb.2014.00567 |
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