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Demonstration of Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by Combining Current Measurements with Detection of Cleavage Fragments

The described methods can be used to investigate the effect of proteases on ion channels, receptors, and other plasma membrane proteins heterologously expressed in Xenopus laevis oocytes. In combination with site-directed mutagenesis, this approach provides a powerful tool to identify functionally r...

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Detalles Bibliográficos
Autores principales:	Krappitz, Matteus, Korbmacher, Christoph, Haerteis, Silke
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2014
Materias:	Biochemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211894/ https://www.ncbi.nlm.nih.gov/pubmed/25045853 http://dx.doi.org/10.3791/51582

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