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The Acetyl Bromide Method Is Faster, Simpler and Presents Best Recovery of Lignin in Different Herbaceous Tissues than Klason and Thioglycolic Acid Methods
We compared the amount of lignin as determined by the three most traditional methods for lignin measurement in three tissues (sugarcane bagasse, soybean roots and soybean seed coat) contrasting for lignin amount and composition. Although all methods presented high reproducibility, major inconsistenc...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212577/ https://www.ncbi.nlm.nih.gov/pubmed/25330077 http://dx.doi.org/10.1371/journal.pone.0110000 |
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author | Moreira-Vilar, Flavia Carolina Siqueira-Soares, Rita de Cássia Finger-Teixeira, Aline de Oliveira, Dyoni Matias Ferro, Ana Paula da Rocha, George Jackson Ferrarese, Maria de Lourdes L. dos Santos, Wanderley Dantas Ferrarese-Filho, Osvaldo |
author_facet | Moreira-Vilar, Flavia Carolina Siqueira-Soares, Rita de Cássia Finger-Teixeira, Aline de Oliveira, Dyoni Matias Ferro, Ana Paula da Rocha, George Jackson Ferrarese, Maria de Lourdes L. dos Santos, Wanderley Dantas Ferrarese-Filho, Osvaldo |
author_sort | Moreira-Vilar, Flavia Carolina |
collection | PubMed |
description | We compared the amount of lignin as determined by the three most traditional methods for lignin measurement in three tissues (sugarcane bagasse, soybean roots and soybean seed coat) contrasting for lignin amount and composition. Although all methods presented high reproducibility, major inconsistencies among them were found. The amount of lignin determined by thioglycolic acid method was severely lower than that provided by the other methods (up to 95%) in all tissues analyzed. Klason method was quite similar to acetyl bromide in tissues containing higher amounts of lignin, but presented lower recovery of lignin in the less lignified tissue. To investigate the causes of the inconsistencies observed, we determined the monomer composition of all plant materials, but found no correlation. We found that the low recovery of lignin presented by the thioglycolic acid method were due losses of lignin in the residues disposed throughout the procedures. The production of furfurals by acetyl bromide method does not explain the differences observed. The acetyl bromide method is the simplest and fastest among the methods evaluated presenting similar or best recovery of lignin in all the tissues assessed. |
format | Online Article Text |
id | pubmed-4212577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42125772014-10-31 The Acetyl Bromide Method Is Faster, Simpler and Presents Best Recovery of Lignin in Different Herbaceous Tissues than Klason and Thioglycolic Acid Methods Moreira-Vilar, Flavia Carolina Siqueira-Soares, Rita de Cássia Finger-Teixeira, Aline de Oliveira, Dyoni Matias Ferro, Ana Paula da Rocha, George Jackson Ferrarese, Maria de Lourdes L. dos Santos, Wanderley Dantas Ferrarese-Filho, Osvaldo PLoS One Research Article We compared the amount of lignin as determined by the three most traditional methods for lignin measurement in three tissues (sugarcane bagasse, soybean roots and soybean seed coat) contrasting for lignin amount and composition. Although all methods presented high reproducibility, major inconsistencies among them were found. The amount of lignin determined by thioglycolic acid method was severely lower than that provided by the other methods (up to 95%) in all tissues analyzed. Klason method was quite similar to acetyl bromide in tissues containing higher amounts of lignin, but presented lower recovery of lignin in the less lignified tissue. To investigate the causes of the inconsistencies observed, we determined the monomer composition of all plant materials, but found no correlation. We found that the low recovery of lignin presented by the thioglycolic acid method were due losses of lignin in the residues disposed throughout the procedures. The production of furfurals by acetyl bromide method does not explain the differences observed. The acetyl bromide method is the simplest and fastest among the methods evaluated presenting similar or best recovery of lignin in all the tissues assessed. Public Library of Science 2014-10-16 /pmc/articles/PMC4212577/ /pubmed/25330077 http://dx.doi.org/10.1371/journal.pone.0110000 Text en © 2014 Moreira-Vilar et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Moreira-Vilar, Flavia Carolina Siqueira-Soares, Rita de Cássia Finger-Teixeira, Aline de Oliveira, Dyoni Matias Ferro, Ana Paula da Rocha, George Jackson Ferrarese, Maria de Lourdes L. dos Santos, Wanderley Dantas Ferrarese-Filho, Osvaldo The Acetyl Bromide Method Is Faster, Simpler and Presents Best Recovery of Lignin in Different Herbaceous Tissues than Klason and Thioglycolic Acid Methods |
title | The Acetyl Bromide Method Is Faster, Simpler and Presents Best Recovery of Lignin in Different Herbaceous Tissues than Klason and Thioglycolic Acid Methods |
title_full | The Acetyl Bromide Method Is Faster, Simpler and Presents Best Recovery of Lignin in Different Herbaceous Tissues than Klason and Thioglycolic Acid Methods |
title_fullStr | The Acetyl Bromide Method Is Faster, Simpler and Presents Best Recovery of Lignin in Different Herbaceous Tissues than Klason and Thioglycolic Acid Methods |
title_full_unstemmed | The Acetyl Bromide Method Is Faster, Simpler and Presents Best Recovery of Lignin in Different Herbaceous Tissues than Klason and Thioglycolic Acid Methods |
title_short | The Acetyl Bromide Method Is Faster, Simpler and Presents Best Recovery of Lignin in Different Herbaceous Tissues than Klason and Thioglycolic Acid Methods |
title_sort | acetyl bromide method is faster, simpler and presents best recovery of lignin in different herbaceous tissues than klason and thioglycolic acid methods |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212577/ https://www.ncbi.nlm.nih.gov/pubmed/25330077 http://dx.doi.org/10.1371/journal.pone.0110000 |
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