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Effects of Human Mesenchymal Stem Cells Isolated from Wharton's Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy Model

Skeletal muscle has good regenerative capacity, but the extent of muscle injury and the developed fibrosis might prevent complete regeneration. The in vivo application of human mesenchymal stem cells (HMSCs) of the umbilical cord and the conditioned media (CM) where the HMSCs were cultured and expan...

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Autores principales: Pereira, T., Armada-da Silva, P. A. S., Amorim, I., Rêma, A., Caseiro, A. R., Gärtner, A., Rodrigues, M., Lopes, M. A., Bártolo, P. J., Santos, J. D., Luís, A. L., Maurício, A. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212633/
https://www.ncbi.nlm.nih.gov/pubmed/25379040
http://dx.doi.org/10.1155/2014/376918
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author Pereira, T.
Armada-da Silva, P. A. S.
Amorim, I.
Rêma, A.
Caseiro, A. R.
Gärtner, A.
Rodrigues, M.
Lopes, M. A.
Bártolo, P. J.
Santos, J. D.
Luís, A. L.
Maurício, A. C.
author_facet Pereira, T.
Armada-da Silva, P. A. S.
Amorim, I.
Rêma, A.
Caseiro, A. R.
Gärtner, A.
Rodrigues, M.
Lopes, M. A.
Bártolo, P. J.
Santos, J. D.
Luís, A. L.
Maurício, A. C.
author_sort Pereira, T.
collection PubMed
description Skeletal muscle has good regenerative capacity, but the extent of muscle injury and the developed fibrosis might prevent complete regeneration. The in vivo application of human mesenchymal stem cells (HMSCs) of the umbilical cord and the conditioned media (CM) where the HMSCs were cultured and expanded, associated with different vehicles to induce muscle regeneration, was evaluated in a rat myectomy model. Two commercially available vehicles and a spherical hydrogel developed by our research group were used. The treated groups obtained interesting results in terms of muscle regeneration, both in the histological and in the functional assessments. A less evident scar tissue, demonstrated by collagen type I quantification, was present in the muscles treated with HMSCs or their CM. In terms of the histological evaluation performed by ISO 10993-6 scoring, it was observed that HMSCs apparently have a long-term negative effect, since the groups treated with CM presented better scores. CM could be considered an alternative to the in vivo transplantation of these cells, as it can benefit from the local tissue response to secreted molecules with similar results in terms of muscular regeneration. Searching for an optimal vehicle might be the key point in the future of skeletal muscle tissue engineering.
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spelling pubmed-42126332014-11-06 Effects of Human Mesenchymal Stem Cells Isolated from Wharton's Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy Model Pereira, T. Armada-da Silva, P. A. S. Amorim, I. Rêma, A. Caseiro, A. R. Gärtner, A. Rodrigues, M. Lopes, M. A. Bártolo, P. J. Santos, J. D. Luís, A. L. Maurício, A. C. Stem Cells Int Research Article Skeletal muscle has good regenerative capacity, but the extent of muscle injury and the developed fibrosis might prevent complete regeneration. The in vivo application of human mesenchymal stem cells (HMSCs) of the umbilical cord and the conditioned media (CM) where the HMSCs were cultured and expanded, associated with different vehicles to induce muscle regeneration, was evaluated in a rat myectomy model. Two commercially available vehicles and a spherical hydrogel developed by our research group were used. The treated groups obtained interesting results in terms of muscle regeneration, both in the histological and in the functional assessments. A less evident scar tissue, demonstrated by collagen type I quantification, was present in the muscles treated with HMSCs or their CM. In terms of the histological evaluation performed by ISO 10993-6 scoring, it was observed that HMSCs apparently have a long-term negative effect, since the groups treated with CM presented better scores. CM could be considered an alternative to the in vivo transplantation of these cells, as it can benefit from the local tissue response to secreted molecules with similar results in terms of muscular regeneration. Searching for an optimal vehicle might be the key point in the future of skeletal muscle tissue engineering. Hindawi Publishing Corporation 2014 2014-10-14 /pmc/articles/PMC4212633/ /pubmed/25379040 http://dx.doi.org/10.1155/2014/376918 Text en Copyright © 2014 T. Pereira et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pereira, T.
Armada-da Silva, P. A. S.
Amorim, I.
Rêma, A.
Caseiro, A. R.
Gärtner, A.
Rodrigues, M.
Lopes, M. A.
Bártolo, P. J.
Santos, J. D.
Luís, A. L.
Maurício, A. C.
Effects of Human Mesenchymal Stem Cells Isolated from Wharton's Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy Model
title Effects of Human Mesenchymal Stem Cells Isolated from Wharton's Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy Model
title_full Effects of Human Mesenchymal Stem Cells Isolated from Wharton's Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy Model
title_fullStr Effects of Human Mesenchymal Stem Cells Isolated from Wharton's Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy Model
title_full_unstemmed Effects of Human Mesenchymal Stem Cells Isolated from Wharton's Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy Model
title_short Effects of Human Mesenchymal Stem Cells Isolated from Wharton's Jelly of the Umbilical Cord and Conditioned Media on Skeletal Muscle Regeneration Using a Myectomy Model
title_sort effects of human mesenchymal stem cells isolated from wharton's jelly of the umbilical cord and conditioned media on skeletal muscle regeneration using a myectomy model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212633/
https://www.ncbi.nlm.nih.gov/pubmed/25379040
http://dx.doi.org/10.1155/2014/376918
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