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Using a non-invasive assessment of lung injury in a murine model of acute lung injury
Arterial oxygen saturation has not been assessed sequentially in conscious mice as a direct consequence of an in vivo murine model of acute lung injury. Here, we report daily changes in arterial oxygen saturation and other cardiopulmonary parameters by using infrared pulse oximetry following intratr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BMJ Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212707/ https://www.ncbi.nlm.nih.gov/pubmed/25478170 http://dx.doi.org/10.1136/bmjresp-2013-000014 |
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author | Lax, Siân Wilson, Michael R Takata, Masao Thickett, David R |
author_facet | Lax, Siân Wilson, Michael R Takata, Masao Thickett, David R |
author_sort | Lax, Siân |
collection | PubMed |
description | Arterial oxygen saturation has not been assessed sequentially in conscious mice as a direct consequence of an in vivo murine model of acute lung injury. Here, we report daily changes in arterial oxygen saturation and other cardiopulmonary parameters by using infrared pulse oximetry following intratracheal lipopolysaccharide (IT-LPS) for up to 9 days, and following IT-phosphate buffered saline up to 72 h as a control. We show that arterial oxygen saturation decreases, with maximal decline at 96 h post IT-LPS. Blood oxygen levels negatively correlate with 7 of 10 quantitative markers of murine lung injury, including neutrophilia and interleukin-6 expression. This identifies infrared pulse oximetry as a method to non-invasively monitor arterial oxygen saturation following direct LPS instillations. |
format | Online Article Text |
id | pubmed-4212707 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BMJ Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-42127072014-12-04 Using a non-invasive assessment of lung injury in a murine model of acute lung injury Lax, Siân Wilson, Michael R Takata, Masao Thickett, David R BMJ Open Respir Res Acute Lung Injury Arterial oxygen saturation has not been assessed sequentially in conscious mice as a direct consequence of an in vivo murine model of acute lung injury. Here, we report daily changes in arterial oxygen saturation and other cardiopulmonary parameters by using infrared pulse oximetry following intratracheal lipopolysaccharide (IT-LPS) for up to 9 days, and following IT-phosphate buffered saline up to 72 h as a control. We show that arterial oxygen saturation decreases, with maximal decline at 96 h post IT-LPS. Blood oxygen levels negatively correlate with 7 of 10 quantitative markers of murine lung injury, including neutrophilia and interleukin-6 expression. This identifies infrared pulse oximetry as a method to non-invasively monitor arterial oxygen saturation following direct LPS instillations. BMJ Publishing Group 2014-01-03 /pmc/articles/PMC4212707/ /pubmed/25478170 http://dx.doi.org/10.1136/bmjresp-2013-000014 Text en Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions This is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 3.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Acute Lung Injury Lax, Siân Wilson, Michael R Takata, Masao Thickett, David R Using a non-invasive assessment of lung injury in a murine model of acute lung injury |
title | Using a non-invasive assessment of lung injury in a murine model of acute lung injury |
title_full | Using a non-invasive assessment of lung injury in a murine model of acute lung injury |
title_fullStr | Using a non-invasive assessment of lung injury in a murine model of acute lung injury |
title_full_unstemmed | Using a non-invasive assessment of lung injury in a murine model of acute lung injury |
title_short | Using a non-invasive assessment of lung injury in a murine model of acute lung injury |
title_sort | using a non-invasive assessment of lung injury in a murine model of acute lung injury |
topic | Acute Lung Injury |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212707/ https://www.ncbi.nlm.nih.gov/pubmed/25478170 http://dx.doi.org/10.1136/bmjresp-2013-000014 |
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