Identification of suitable reference genes for gene expression studies by qRT-PCR in the blister beetle Mylabris cichorii

The blister beetle Mylabris cichorii L. (Coleoptera: Meloidae) is a traditional medicinal insect recorded in the Chinese Pharmacopoeia. It synthesizes cantharidin, which kills cancer cells efficiently. Only males produce large amounts of cantharidin. Reference genes are required as endogenous contro...

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Autores principales: Wang, Yu, Wang, Zhong-Kang, Huang, Yi, Liao, Yu-Feng, Yin, You-Ping, Tu, Jake
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212844/
https://www.ncbi.nlm.nih.gov/pubmed/25368050
http://dx.doi.org/10.1093/jis/14.1.94
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author Wang, Yu
Wang, Zhong-Kang
Huang, Yi
Liao, Yu-Feng
Yin, You-Ping
Tu, Jake
author_facet Wang, Yu
Wang, Zhong-Kang
Huang, Yi
Liao, Yu-Feng
Yin, You-Ping
Tu, Jake
author_sort Wang, Yu
collection PubMed
description The blister beetle Mylabris cichorii L. (Coleoptera: Meloidae) is a traditional medicinal insect recorded in the Chinese Pharmacopoeia. It synthesizes cantharidin, which kills cancer cells efficiently. Only males produce large amounts of cantharidin. Reference genes are required as endogenous controls for the analysis of differential gene expression in M. cichorii . Our study chose 10 genes as candidate reference genes. The stability of expression of these genes was analyzed by quantitative PCR and determined with two algorithms, geNorm and Normfinder. We recommend UBE3A and RPL22e as suitable reference genes in females and UBE3A, TAF5, and RPL22e in males.
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spelling pubmed-42128442014-10-31 Identification of suitable reference genes for gene expression studies by qRT-PCR in the blister beetle Mylabris cichorii Wang, Yu Wang, Zhong-Kang Huang, Yi Liao, Yu-Feng Yin, You-Ping Tu, Jake J Insect Sci Papers The blister beetle Mylabris cichorii L. (Coleoptera: Meloidae) is a traditional medicinal insect recorded in the Chinese Pharmacopoeia. It synthesizes cantharidin, which kills cancer cells efficiently. Only males produce large amounts of cantharidin. Reference genes are required as endogenous controls for the analysis of differential gene expression in M. cichorii . Our study chose 10 genes as candidate reference genes. The stability of expression of these genes was analyzed by quantitative PCR and determined with two algorithms, geNorm and Normfinder. We recommend UBE3A and RPL22e as suitable reference genes in females and UBE3A, TAF5, and RPL22e in males. Oxford University Press 2014-07-18 /pmc/articles/PMC4212844/ /pubmed/25368050 http://dx.doi.org/10.1093/jis/14.1.94 Text en This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, providedthe original work is properly cited.
spellingShingle Papers
Wang, Yu
Wang, Zhong-Kang
Huang, Yi
Liao, Yu-Feng
Yin, You-Ping
Tu, Jake
Identification of suitable reference genes for gene expression studies by qRT-PCR in the blister beetle Mylabris cichorii
title Identification of suitable reference genes for gene expression studies by qRT-PCR in the blister beetle Mylabris cichorii
title_full Identification of suitable reference genes for gene expression studies by qRT-PCR in the blister beetle Mylabris cichorii
title_fullStr Identification of suitable reference genes for gene expression studies by qRT-PCR in the blister beetle Mylabris cichorii
title_full_unstemmed Identification of suitable reference genes for gene expression studies by qRT-PCR in the blister beetle Mylabris cichorii
title_short Identification of suitable reference genes for gene expression studies by qRT-PCR in the blister beetle Mylabris cichorii
title_sort identification of suitable reference genes for gene expression studies by qrt-pcr in the blister beetle mylabris cichorii
topic Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212844/
https://www.ncbi.nlm.nih.gov/pubmed/25368050
http://dx.doi.org/10.1093/jis/14.1.94
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