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Analysis of intestinal bacterial community diversity of adult Dastarcus helophoroides

Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), and a culture-dependent technique were used to study the diversity of the intestinal bacterial community in adult Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae). Universal bacterial primers targeting 200 b...

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Autores principales: Zhang, Z. Q., He, C., Li, M. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212848/
https://www.ncbi.nlm.nih.gov/pubmed/25200108
http://dx.doi.org/10.1093/jis/14.1.114
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author Zhang, Z. Q.
He, C.
Li, M. L.
author_facet Zhang, Z. Q.
He, C.
Li, M. L.
author_sort Zhang, Z. Q.
collection PubMed
description Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), and a culture-dependent technique were used to study the diversity of the intestinal bacterial community in adult Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae). Universal bacterial primers targeting 200 bp regions of the 16S rDNA gene were used in the PCR-DGGE assay, and 14 bright bands were obtained. The intestinal bacteria detected by PCR-DGGE were classified to Enterococcus (Lactobacillales: Enterococcaceae), Bacillus (Bacillales: Bacillaceae), Cellvibrio (Pseudomonadales: Pseudomonadaceae), Caulobacter (Caulobacterales: Caulobacteraceae), and uncultured bacteria, whereas those isolated by the culture-dependent technique belonged to Staphylococcus (Bacillales: Staphylococcaceae), Pectobacterium Enterobacteriales: Enterobacteriaceae), and Enterobacter (Enterobacteriales: Enterobacteriaceae). These intestinal bacteria represented the groups Lactobacillales ( Enterococcus ), Pseudomonadales ( Cellvibrio ), Caulobacterales ( Caulobacter ), Bacilli ( Bacillus and Staphylococcus ), and Gammaproteobacteria ( Pectobacterium and Enterobacter ). Our results demonstrated that PCR-DGGE analysis and the culture-dependent technique were useful in determining the intestinal bacteria of D. helophoroides and the two methods should be integrated to characterize the microbial community and diversity.
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spelling pubmed-42128482014-10-31 Analysis of intestinal bacterial community diversity of adult Dastarcus helophoroides Zhang, Z. Q. He, C. Li, M. L. J Insect Sci Papers Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), and a culture-dependent technique were used to study the diversity of the intestinal bacterial community in adult Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae). Universal bacterial primers targeting 200 bp regions of the 16S rDNA gene were used in the PCR-DGGE assay, and 14 bright bands were obtained. The intestinal bacteria detected by PCR-DGGE were classified to Enterococcus (Lactobacillales: Enterococcaceae), Bacillus (Bacillales: Bacillaceae), Cellvibrio (Pseudomonadales: Pseudomonadaceae), Caulobacter (Caulobacterales: Caulobacteraceae), and uncultured bacteria, whereas those isolated by the culture-dependent technique belonged to Staphylococcus (Bacillales: Staphylococcaceae), Pectobacterium Enterobacteriales: Enterobacteriaceae), and Enterobacter (Enterobacteriales: Enterobacteriaceae). These intestinal bacteria represented the groups Lactobacillales ( Enterococcus ), Pseudomonadales ( Cellvibrio ), Caulobacterales ( Caulobacter ), Bacilli ( Bacillus and Staphylococcus ), and Gammaproteobacteria ( Pectobacterium and Enterobacter ). Our results demonstrated that PCR-DGGE analysis and the culture-dependent technique were useful in determining the intestinal bacteria of D. helophoroides and the two methods should be integrated to characterize the microbial community and diversity. Oxford University Press 2014-08-12 /pmc/articles/PMC4212848/ /pubmed/25200108 http://dx.doi.org/10.1093/jis/14.1.114 Text en This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, providedthe original work is properly cited.
spellingShingle Papers
Zhang, Z. Q.
He, C.
Li, M. L.
Analysis of intestinal bacterial community diversity of adult Dastarcus helophoroides
title Analysis of intestinal bacterial community diversity of adult Dastarcus helophoroides
title_full Analysis of intestinal bacterial community diversity of adult Dastarcus helophoroides
title_fullStr Analysis of intestinal bacterial community diversity of adult Dastarcus helophoroides
title_full_unstemmed Analysis of intestinal bacterial community diversity of adult Dastarcus helophoroides
title_short Analysis of intestinal bacterial community diversity of adult Dastarcus helophoroides
title_sort analysis of intestinal bacterial community diversity of adult dastarcus helophoroides
topic Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212848/
https://www.ncbi.nlm.nih.gov/pubmed/25200108
http://dx.doi.org/10.1093/jis/14.1.114
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