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Analysis of intestinal bacterial community diversity of adult Dastarcus helophoroides
Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), and a culture-dependent technique were used to study the diversity of the intestinal bacterial community in adult Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae). Universal bacterial primers targeting 200 b...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212848/ https://www.ncbi.nlm.nih.gov/pubmed/25200108 http://dx.doi.org/10.1093/jis/14.1.114 |
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author | Zhang, Z. Q. He, C. Li, M. L. |
author_facet | Zhang, Z. Q. He, C. Li, M. L. |
author_sort | Zhang, Z. Q. |
collection | PubMed |
description | Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), and a culture-dependent technique were used to study the diversity of the intestinal bacterial community in adult Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae). Universal bacterial primers targeting 200 bp regions of the 16S rDNA gene were used in the PCR-DGGE assay, and 14 bright bands were obtained. The intestinal bacteria detected by PCR-DGGE were classified to Enterococcus (Lactobacillales: Enterococcaceae), Bacillus (Bacillales: Bacillaceae), Cellvibrio (Pseudomonadales: Pseudomonadaceae), Caulobacter (Caulobacterales: Caulobacteraceae), and uncultured bacteria, whereas those isolated by the culture-dependent technique belonged to Staphylococcus (Bacillales: Staphylococcaceae), Pectobacterium Enterobacteriales: Enterobacteriaceae), and Enterobacter (Enterobacteriales: Enterobacteriaceae). These intestinal bacteria represented the groups Lactobacillales ( Enterococcus ), Pseudomonadales ( Cellvibrio ), Caulobacterales ( Caulobacter ), Bacilli ( Bacillus and Staphylococcus ), and Gammaproteobacteria ( Pectobacterium and Enterobacter ). Our results demonstrated that PCR-DGGE analysis and the culture-dependent technique were useful in determining the intestinal bacteria of D. helophoroides and the two methods should be integrated to characterize the microbial community and diversity. |
format | Online Article Text |
id | pubmed-4212848 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-42128482014-10-31 Analysis of intestinal bacterial community diversity of adult Dastarcus helophoroides Zhang, Z. Q. He, C. Li, M. L. J Insect Sci Papers Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), and a culture-dependent technique were used to study the diversity of the intestinal bacterial community in adult Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae). Universal bacterial primers targeting 200 bp regions of the 16S rDNA gene were used in the PCR-DGGE assay, and 14 bright bands were obtained. The intestinal bacteria detected by PCR-DGGE were classified to Enterococcus (Lactobacillales: Enterococcaceae), Bacillus (Bacillales: Bacillaceae), Cellvibrio (Pseudomonadales: Pseudomonadaceae), Caulobacter (Caulobacterales: Caulobacteraceae), and uncultured bacteria, whereas those isolated by the culture-dependent technique belonged to Staphylococcus (Bacillales: Staphylococcaceae), Pectobacterium Enterobacteriales: Enterobacteriaceae), and Enterobacter (Enterobacteriales: Enterobacteriaceae). These intestinal bacteria represented the groups Lactobacillales ( Enterococcus ), Pseudomonadales ( Cellvibrio ), Caulobacterales ( Caulobacter ), Bacilli ( Bacillus and Staphylococcus ), and Gammaproteobacteria ( Pectobacterium and Enterobacter ). Our results demonstrated that PCR-DGGE analysis and the culture-dependent technique were useful in determining the intestinal bacteria of D. helophoroides and the two methods should be integrated to characterize the microbial community and diversity. Oxford University Press 2014-08-12 /pmc/articles/PMC4212848/ /pubmed/25200108 http://dx.doi.org/10.1093/jis/14.1.114 Text en This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, providedthe original work is properly cited. |
spellingShingle | Papers Zhang, Z. Q. He, C. Li, M. L. Analysis of intestinal bacterial community diversity of adult Dastarcus helophoroides |
title |
Analysis of intestinal bacterial community diversity of adult
Dastarcus helophoroides |
title_full |
Analysis of intestinal bacterial community diversity of adult
Dastarcus helophoroides |
title_fullStr |
Analysis of intestinal bacterial community diversity of adult
Dastarcus helophoroides |
title_full_unstemmed |
Analysis of intestinal bacterial community diversity of adult
Dastarcus helophoroides |
title_short |
Analysis of intestinal bacterial community diversity of adult
Dastarcus helophoroides |
title_sort | analysis of intestinal bacterial community diversity of adult
dastarcus helophoroides |
topic | Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212848/ https://www.ncbi.nlm.nih.gov/pubmed/25200108 http://dx.doi.org/10.1093/jis/14.1.114 |
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